Automation of glucose measurement in fermentor broths

Abstract

A semi-on-line automated analysis for fermentation is presented. The system consists o f a modified commercial biosensor analyser YSI 27 and a Gilson dilutor injector. The results of direct glucose monitoring in fermentation broths are presented. Measurements, calibrations and washes are completely automated, with a m a x i m u m analysis frequency o f 16 per hour with dilutions. The detection limits are 50 mg/1 and 41 g/1 with g o o d linearity and a precision o f ___ 5%.

Principles and Applications of Biosensors for Bioprocess Monitoring and Control

“…Specific areas of critical importance where on-line process monitoring can prove to be beneficial include (1) production of recombinant DNA microorganisms (bacteria, yeast, and mammalian cell cuItures) with expression systems for heterologous proteins that require careful control of operational conditions in order to prevent the formation of refractile bodies (insoluble protein products) or instability of the proteins; (2) monoclonal antibody (Mab) production in hybridoma cultures where control of key nutrients, for example, glucose and glutamine is essential to maintain high cell viability and Mab production; and (3) computer control using novel strategies such as adaptive control or neural networks where a lack of suitable sensors can limit the testing of such applications (Andersen and Gruenberg, 1987;Strohl et al, 1986;Lelong et al, 1991;Johansen et al, 1992).…”