Autonomic regulation of secretion and transductal fluxes of ions in the rat parotid

Mangos, J. A.; McSherry, NR; Arvanitakis, SN

doi:10.1152/ajplegacy.1973.225.3.683

Cited by 32 publications

(11 citation statements)

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“…lt should be noted that the decline in the secretory response following attainment of maximum rates of secretion is far more rapid than could reasonably be expected on the basis of falling plasma concentrations; a similar phenomenon seen during continuous acetylcholine stimulation has been interpreted as due to some form of gland desensitization brought about by continuous stimulation (Case et al, 1979). in the parotid gland the maximum flow response was not more than 20% of what can be elicited with pilocarpinc, carhachol or acetyicholine (Mangos and Braun, 1966;Mangos et «/., 1973: Young andvan Lennep, 1979) but was of similar magnitude to that evoked by isoproterenol (Mangos et al, 1973). It is clear that, ofthe three major salivary glands ofthe rat.…”

Section: Flow Responsesmentioning

confidence: 89%

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The Effect of Phenylephrine on Excretion of Fluid and Electrolytes by the Parotid and Mandibular Glands of the Rat

Chen

Jones

et al. 1979

Aust J Exp Biol Med

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Summary. The effect has been investigated of the a-adrenergtc agonist, phenylephrine, on excretion of water and electrolytes (Na, K, and HCO.,) by the parotid and mandibular glands of the rat. In the mandibular glands the agonist was as elTective as acetylcholine (or parasympaihetic nerve stimulation) in stimulating secretion, and ihe electrolyte excretory patterns seen in the two modes of stimulation were similar. In the parotid gland, phenytephrine was only one-fifth as potent as acetylcholine (or parasympathctic nerve stimulation) in evoking a secretory response but, when due allowance for now rate differences is made, the electrolyte excretion patterns were similar. In both glands the secretory response to phenylephrine was totally difTcrcnl. in magnitude and in electrolyte excretion pattern, to that evoked by the ^-adrenergic agunisi, isoprenaline. It is concltidcd, as has already been established for secretion of exportable protein, ihat a-adrcnergic agonists have very similar effecis to miiscarinic agonists both on endpiecc and on duct cells and thai these actions are completely different from those evoked by activation of,^-adrenergic receptors.

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Section: Flow Responsesmentioning

confidence: 89%

“…1975). One study on the effect of isoprenaline on the rat parotid has been published (Mangos, McSherry and Arvanitakis, 1973), and it is known that this drug does not cause secretion by the rat sublingual gland (Martin and Young. 1971a).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Phenylephrine on Excretion of Fluid and Electrolytes by the Parotid and Mandibular Glands of the Rat

Chen

Jones

et al. 1979

Aust J Exp Biol Med

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“…Preparation of the animals for micropuncture and saliva collections were as previously described by these authors for ferrets and rats. 1,2,4 In order to study the net water fluxes of the lobular and main ducts of these salivary glands, we used the method of split-oil droplet stationary microperfusion technique originally developed by Shipp et al method has been used in this laboratory for the study of the chloride and water fluxes in the interlobular ducts of the rat pancreas.7…”

Section: Methodsmentioning

confidence: 99%

Transductal Fluxes of Water and Monovalent Ions in Ferret Salivary Glands

et al. 1981

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The net transductal fluxes of water and monovalent ions were measured in the parotid and submandibular salivary glands of the ferret, Mustela putorius furo, during stimulation of secretion with pilocarpine. The duct systems of these glands were found to be impermeable to water using the split-oil droplet method for stationary microperfusion of lobular and main ducts. The net transductal fluxes of Na+, K+, Cl-, and HCO3- were characterized by analysis of ductal fluid samples obtained simultaneously from the intercalated, lobular, and main ducts of these glands.

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“…Salivary intra-and extralobular ducts are considered to be water impermeable (e.g., Mangos et al, 1973;Cook and Young, 1989) and A5 cells share immunological characteristics with such ducts in the rat submandibular gland. Further, two studies Li et al, 1994) ies and venules.…”

Section: Examination Of Salivary Cells For Aqpl Expressionmentioning

confidence: 99%

“…Although the experimental results are clear, they are also physiologically somewhat surprising. Salivary intra-and extralobular ducts are considered to be water impermeable (e.g., Mangos et al, 1973;Cook and Young, 1989) and A5 cells share immunological characteristics with such ducts in the rat submandibular gland. Further, two studies Li et al, 1994) have demonstrated that AQPl is not expressed by any epithelial cells (ductal and acinar) in this gland.…”

Section: Examination Of Salivary Cells For Aqpl Expressionmentioning

confidence: 99%

Evidence for the presence of a Hg‐inhibitable water‐permeability pathway and aquaporin 1 in A5 salivary epithelial cells

Lazowski

Delporte

et al. 1995

Journal Cellular Physiology

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The molecular basis of water-permeability in salivary and other exocrine glands is not understood. We have examined two well-studied salivary epithelial cell lines for evidence of a Hg-inhibitable water-permeability pathway. A5 and HSG cells are derived from rat and human submandibular glands, respectively. Only A5 cells demonstrated such a pathway. The rate of A5 cell osmotic shrinkage was inhibited about fivefold in the presence of 300 microM HgCl2. To determine if this activity was associated with the expression of the prototypical water channel (aquaporin, AQP) AQP1, we used three separate experimental approaches; Northern analysis and reverse transcription-polymerase chain reaction (RT-PCR) analysis of isolated mRNA, and Western analysis of cell membranes. All three methods yielded positive results with A5 cells and negative results with HSG cells. The approximately 800 bp product of RT-PCR was analyzed further by sequencing and restriction enzyme digestion. The results were consistent with the previously reported coding region sequence for rat kidney AQP1. The aggregate data demonstrate that marked differences in water-permeability and water channel expression exist in these two salivary epithelial cell lines.

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Autonomic regulation of secretion and transductal fluxes of ions in the rat parotid

Cited by 32 publications

References 0 publications

The Effect of Phenylephrine on Excretion of Fluid and Electrolytes by the Parotid and Mandibular Glands of the Rat

The Effect of Phenylephrine on Excretion of Fluid and Electrolytes by the Parotid and Mandibular Glands of the Rat

Transductal Fluxes of Water and Monovalent Ions in Ferret Salivary Glands

Evidence for the presence of a Hg‐inhibitable water‐permeability pathway and aquaporin 1 in A5 salivary epithelial cells

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