Autonomous and non-autonomous Tn3-family transposons and their role in the evolution of mobile genetic elements

Szuplewska, Magdalena; Czarnecki, Jakub; Bartosik, Dariusz

doi:10.1080/2159256x.2014.998537

Cited by 21 publications

(12 citation statements)

References 29 publications

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“…This indicated that movement of bla PAU-1 was mediated by a Tn 3 -like transposon. The Tn 3 -like family transposons are widely distributed among prokaryotes 20. However, the Tn 3 -like transposon structure most similar to the pICP-4GES transposon in public databases was located on the Acidovorax sp.…”

Section: Resultsmentioning

confidence: 99%

PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical Pseudomonas aeruginosa Isolate

Jian

Ying

et al. 2019

IDR

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PurposeThe aim of this work was to identify a novel β-lactamase gene blaPAU-1 encoded on the plasmid of a clinical Pseudomonas aeruginosa isolate.Materials and methodsThe clinical P. aeruginosa isolates were isolated from a hospital in southern China. Molecular cloning was performed to analyze the function of the resistance gene. The minimum inhibitory concentration (MIC) was determined by means of the agar dilution method to determine the antimicrobial susceptibilities of the strains. Whole-genome sequencing and comparative genomics analysis were performed to analyze the structures of the resistance gene-related sequences.ResultsPAU-1 is a molecular class A, Bush-Jacoby group 2be enzyme which encoded 293 amino acids and shared 74% amino acid identity with a putative class A β-lactamase from Rhodoferax saidenbachensis. Cloned blaPAU-1 in Escherichia coli and P. aeruginosa conferred resistance to piperacillin and ampicillin, and elevated the MIC with a 2–3 dilution for some oxyimino-β-lactams in P. aeruginosa. The genetic environment of blaPAU-1 is tnpA-res-hp-relE-blaPAU-1-lysR, which is in accordance with the structure of a Tn3 transposon. Epidemiological investigation of blaPAU-1 in the same district did not show any evidences of molecular dissemination associated with this determinant.ConclusionA novel class A β-lactamase gene, blaPAU-1, associated with the mobile genetic element was identified on a transferable plasmid in a clinical P. aeruginosa isolate. Strict surveillance for the emergence of the new determinant should be established and an effort should be made to block the dissemination of this determinant.

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Section: Resultsmentioning

confidence: 99%

PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical Pseudomonas aeruginosa Isolate

Jian

Ying

et al. 2019

IDR

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“…Phyla that were less abundant than 1% in both datasets were not included. (Szuplewska et al, 2015). It has to be noted that sequences associated with such transposons were not identified in the studied plasmidome.…”

Section: Integrons Transposons and Insertion Sequencesmentioning

confidence: 95%

First Report on the Plasmidome From a High-Altitude Lake of the Andean Puna

et al. 2020

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Mobile genetic elements, including plasmids, drive the evolution of prokaryotic genomes through the horizontal transfer of genes allowing genetic exchange between bacteria. Moreover, plasmids carry accessory genes, which encode functions that may offer an advantage to the host. Thus, it is expected that in a certain ecological niche, plasmids are enriched in accessory functions, which are important for their hosts to proliferate in that niche. Puquio de Campo Naranja is a high-altitude lake from the Andean Puna exposed to multiple extreme conditions, including high UV radiation, alkalinity, high concentrations of arsenic, heavy metals, dissolved salts, high thermal amplitude and low O 2 pressure. Microorganisms living in this lake need to develop efficient mechanisms and strategies to cope under these conditions. The aim of this study was to characterize the plasmidome of microbialites from Puquio de Campo Naranja, and identify potential hosts and encoded functions using a deep-sequencing approach. The potential ecological impact of the plasmidome, including plasmids from cultivable and non-cultivable microorganisms, is described for the first time in a lake representing an extreme environment of the Puna. This study showed that the recovered genetic information for the plasmidome was novel in comparison to the metagenome derived from the same environment. The study of the total plasmid population allowed the identification of genetic features typically encoded by plasmids, such as resistance and virulence factors. The resistance genes comprised resistances to heavy metals, antibiotics and stress factors. These results highlight the key role of plasmids for their hosts and impact of extrachromosomal elements to thrive in a certain ecological niche.

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“…For ICEs, excision and circularization represent key steps in the mobilization process [ 33 ]. We used a specifically developed PCR assay to demonstrate the presence of ICEHo-I and -II in their episomal circularized forms across many isolates in our screened cohort.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element

et al. 2020

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Background Mobile genetic elements are found in genomes throughout the microbial world, mediating genome plasticity and important prokaryotic phenotypes. Even the cell wall-less mycoplasmas, which are known to harbour a minimal set of genes, seem to accumulate mobile genetic elements. In Mycoplasma hominis, a facultative pathogen of the human urogenital tract and an inherently very heterogeneous species, four different MGE-classes had been detected until now: insertion sequence ISMhom-1, prophage MHoV-1, a tetracycline resistance mediating transposon, and ICEHo, a species-specific variant of a mycoplasma integrative and conjugative element encoding a T4SS secretion system (termed MICE). Results To characterize the prevalence of these MGEs, genomes of 23 M. hominis isolates were assembled using whole genome sequencing and bioinformatically analysed for the presence of mobile genetic elements. In addition to the previously described MGEs, a new ICEHo variant was found, which we designate ICEHo-II. Of 15 ICEHo-II genes, five are common MICE genes; eight are unique to ICEHo-II; and two represent a duplication of a gene also present in ICEHo-I. In 150 M. hominis isolates and based on a screening PCR, prevalence of ICEHo-I was 40.7%; of ICEHo-II, 28.7%; and of both elements, 15.3%. Activity of ICEHo-I and -II was demonstrated by detection of circularized extrachromosomal forms of the elements through PCR and subsequent Sanger sequencing. Conclusions Nanopore sequencing enabled the identification of mobile genetic elements and of ICEHo-II, a novel MICE element of M. hominis, whose phenotypic impact and potential impact on pathogenicity can now be elucidated.

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Autonomous and non-autonomous Tn3-family transposons and their role in the evolution of mobile genetic elements

Cited by 21 publications

References 29 publications

<p>PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical <em>Pseudomonas aeruginosa</em> Isolate</p>

<p>PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical <em>Pseudomonas aeruginosa</em> Isolate</p>

First Report on the Plasmidome From a High-Altitude Lake of the Andean Puna

Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element

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