Electric bicycles, motorcycles, bicycles and other two-wheelers have incomparable convenience in short trips, but as vulnerable road users, the probability of accidents in complex traffic environments is higher than that of other vehicles. Based on the analysis of CIDAS accident data, this paper calculates the types of traffic participants in traffic accidents in which the passenger car is the main vehicle, and analyzes the two common traffic accident scenarios that are easy to cause injury to the two-wheeled vehicle. The basic principle, test scenario, test method and evaluation method of two ADAS functions, rear traffic crossing warning and door opening warning, are introduced according to the new C-NCAP protocol of 2024 edition. And the validation test results of three vehicle models were selected for analysis, and the final conclusion was drawn.