1997
DOI: 10.1006/fgbi.1997.0980
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Autonomous Plasmid Replication inAspergillus nidulans:AMA1 and MATE Elements

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Cited by 112 publications
(87 citation statements)
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“…A swoF pyrG strain was transformed with a genomic library constructed in vector pRG3AMA1, which carries the pyr4 gene and the AMA1 sequence for plasmid autonomous replication (1,36). A total of 1,092 pyr prototrophs were screened for growth at restrictive temperature (42°C).…”
Section: Allelesmentioning
confidence: 99%
“…A swoF pyrG strain was transformed with a genomic library constructed in vector pRG3AMA1, which carries the pyr4 gene and the AMA1 sequence for plasmid autonomous replication (1,36). A total of 1,092 pyr prototrophs were screened for growth at restrictive temperature (42°C).…”
Section: Allelesmentioning
confidence: 99%
“…AMA1 containing vectors have been designed and can be used for cotransformation with genomic libraries or for the new original instant bank procedure to isolate genes . However, the AMA1 bearing vectors work as replicating plasmids only with some fungi (Aleksenko and Clutterbuck, 1997).…”
Section: Academic Pressmentioning
confidence: 99%
“…It was recovered from a genomic library of A. nidulans during a search for an element that increased transformation efficiency (Gems et al 1991). AMA1-bearing vectors generally have the following properties: (a) a high transformation efficiency that results because these vectors, unlike genomeintegrating vectors, obviate the need for integration into the fungal genome, (b) these vectors localize to nuclei of fungal cells, (c) in Southern blot analysis, these vectors extracted from fungal transformants are detected as bands with electrophoretic mobility similar or identical to circular and/or linear forms of the vector extracted from Escherichia coli, (d) these vectors tend to be lost from fungal lineages under non-selective conditions, and (e) these vectors can be recovered as circular plasmids from E. coli transformed with DNA extract from fungal transformants (Aleksenko and Clutterbuck 1997).…”
Section: Introductionmentioning
confidence: 99%