Autophagy and mitochondrial damage in the testis of high-fat diet fed rats

Latino, Debora; Baccari, Gabriella Chieffi; Fiore, Maria Maddalena Di; Cioffi, Federica; Venditti, Massimo; Giacco, Antonia; Santillo, Alessandra

doi:10.1016/j.ygcen.2022.114104

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…The HFD-fed mice had an increased protein expression of LC3B-II, indicating that HFD feeding induced autophagy. These results were in line with those of Latino et al 39 and Mu et al 18 ; Mu et al also observed elevated protein expression of Beclin 1 in the semen of infertile obese men. In addition, an HFD-induced diabetic model demonstrated dual roles of autophagy in testis function; increased protein expression of Beclin 1 and the ratio of LC3-I/LC3-II in Sertoli cells and decreased expression of these proteins in Leydig cells were observed.…”

Section: Discussionsupporting

confidence: 90%

Hirsutella sinensis intensifies testicular function and spermatogenesis in male mice with high-fat diet-induced obesity

Liu,

Chen,

Chiang

et al. 2024

Journal of the Chinese Medical Association

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Background: Hirsutella sinensis (HS) is a mycelium isolated from the fruiting body of the medicinal mushroom Cordyceps sinensis. This study explored whether HS treatment affects reproductive dysfunction in a high-fat diet (HFD)-induced mouse model and regulates various mechanisms, focusing on oxidative stress, apoptosis, inflammation, and autophagy. Methods: Twenty-four C57BL/6J (B6) mice were randomly divided into a standard chow diet (NCD)- or HFD-fed group for 24 weeks. During the final 8 weeks, half of the HFD-fed mice were orally administered HS (HFD+HS). Biochemical markers, including glucose, insulin, triglycerides, and total cholesterol, were assessed, and hormones, including testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH), were analyzed. Liver and testicular histology, as well as sperm quality markers such as sperm motility, sperm count, and percentage of sperm with normal morphology, were observed. The activities of the testicular antioxidants superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) and the products of lipid peroxidation, such as MDA, were measured. The protein expression levels of apoptosis-, autophagy- and inflammation-related markers were measured. Results: The HFD-fed mice had abnormal sex hormone levels, poor sperm quality, and a destroyed testicular structure, with increased oxidative stress and apoptosis in the testis. HS supplementation in HFD-fed mice attenuated testicular apoptosis by suppressing the Bax/Bcl-xl ratio and cleaved caspase 3 protein expression. The HS-treated mice exhibited improved reproductive function, possibly due to reduced oxidative stress and apoptosis, suggesting that HS has a protective effect against HFD-induced testicular damage. Conclusion: Male mice supplemented with HS exhibited attenuated poor semen quality and reduced testosterone levels brought about by high-fat diet-induced obesity by reducing oxidative stress.

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Section: Discussionsupporting

confidence: 90%

Hirsutella sinensis intensifies testicular function and spermatogenesis in male mice with high-fat diet-induced obesity

Liu,

Chen,

Chiang

et al. 2024

Journal of the Chinese Medical Association

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“…Our results showed an increase in the levels of MDA and a decrease in the expression of the antioxidant enzyme SOD1 in all three regions of the rats in the HFD group relative to that in the rats of the control group. These results indicated an increase in oxidative stress and matched the findings of previous studies conducted on HFD rats, indicating that HFD causes cellular oxidative stress in the testis (Latino et al, 2022; Verderame et al, 2018; Migliaccio, Sica et al, 2019; Migliaccio, Gregorio et al, 2019). Exposure to oxidative stress due to the administration of an HFD can reduce sperm fertility in obese mice (Y. Li et al, 2022).…”

Section: Discussionsupporting

confidence: 89%

“…A high-fat diet (HFD) affects reproductive performance (Crean & Senior, 2019), and until now many studies have investigated the impairment of testis function (Matuszewska et al, 2020;Migliaccio, Sica et al, 2019;Migliaccio, Gregorio et al, 2019;Verderame et al, 2018). In this regard, our research group demonstrated recently that autophagy and apoptosis were activated in the testis of HFD rats to cope with high oxidative stress and also found that mitochondrial compartments are damaged by an HFD (Latino et al, 2022). On the other hand, it is generically recognized that the cellular oxidative stress is the major cause of damage to reproductive functions (Aitken et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Effects of a high‐fat diet on rat epididymis

et al. 2023

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The epididymis plays an essential role in reproduction, promoting sperm cell maturation. In this study, we investigated the effects of a high-fat diet (HFD) in the three regions of the epididymis of rats, including caput, corpus, and cauda. Our results showed an increase in malondialdehyde and a decrease in superoxide dismutase, which indicated an increase in oxidative stress in all segments of the epididymis. The cellular response mechanisms were mostly detected in the corpus/ cauda regions, which showed an increase in apoptosis, probably for eliminating dysfunctional cells arising from HFD-induced oxidative stress, and a decrease in mitophagy. Additionally, an increase in lipophagy to prevent lipid accumulation and a decrease in cell proliferation were recorded in the corpus.

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“…A diet rich in fat was previously reported to alter carbohydrate metabolism and reduce cell proliferation, eventually affecting spermatogenesis 30 . It was also reported to induce mitochondrial and testicular damage 31, 32 . Hyperinsulinemia and decreased insulin sensitivity due to high-fat diet consumption were reported to be the probable cause of Leydig cell dysfunction and testicular damage 33 .…”

Section: Histological Studymentioning

confidence: 99%

Aloe vera Gel Ameliorates Fat-Rich and High Fructose (FRHF) Diet-Induced Pancreatic and Splenic Damage in Mice

Dibal,

Goni,

Attah

et al. 2023

Borneo J Pharm

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High-fat diet alone or in combination with high fructose has been known to induce diabetes, obesity, hypertension, and immune dysfunction. The study evaluates the role of Aloe vera in fat-rich and high fructose diet-induced (FRHFD) hyperglycemias in addition to testicular and splenic morphology in mice. Twenty BALB/c Mice were randomly distributed into four groups (n=5). The groups were fed on a normal diet, FRHFD, FRHFD + 10 g A. vera, and FRHFD + 20 g A. vera for 10 weeks. All the mice were sacrificed a day after the 10 weeks of treatment. The result showed that mice fed on FRHFD plus A. vera had a significantly lower (p<0.05) blood glucose level relative to the FRHFD-fed mice. The mice fed on FRHFD plus A. vera had a significantly lower (p<0.05) blood glucose level relative to the FRHFD-fed mice. Aloe vera was found to ameliorate FRHFD-induced pancreatic islet and acini damage. It also prevented distorted lymphoid cells and testicular damage induced by FRHFD. Aloe vera prevents hyperglycemia and protects pancreatic islets in FRHFD-fed mice. It further prevents immune dysfunction and protects against testicular damage. Hence, A. vera supplementation could be an alternative and/or complementary therapy for hyperglycemia-related disorders.

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Autophagy and mitochondrial damage in the testis of high-fat diet fed rats

Cited by 6 publications

References 47 publications

Hirsutella sinensis intensifies testicular function and spermatogenesis in male mice with high-fat diet-induced obesity

Hirsutella sinensis intensifies testicular function and spermatogenesis in male mice with high-fat diet-induced obesity

Effects of a high‐fat diet on rat epididymis

Aloe vera Gel Ameliorates Fat-Rich and High Fructose (FRHF) Diet-Induced Pancreatic and Splenic Damage in Mice

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