Autophagy induced by hypoxia in pulpitis is mediated by HIF-1α/BNIP3

Wang, Xiaohe; Wu, Zeyu; Zhang, Yangyang; Lian, Bingjie; Ma, Li; Zhao, Jin

doi:10.1016/j.archoralbio.2024.105881

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…Several literatures have revealed that the upstream pathway regulating autophagy is mainly involved in HIF-1α/mTOR, AMPK/mTOR, and AKT/mTOR in macrophages [ 31 – 33 ]. We firstly detected the phosphorylation of mTOR and found that β-elemene treatment significantly prevented the expression of p-mTOR/mTOR in LPS-and IFN-γ-induced BV-2 cells (Fig.…”

Section: β-Elemene Prevented the Activation Of Akt/mtor Signaling Pat...mentioning

confidence: 99%

β-elemene promotes microglial M2-like polarization against ischemic stroke via AKT/mTOR signaling axis-mediated autophagy

Zhao,

Chen,

Zhang

et al. 2024

Chin Med

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Background Resident microglia- and peripheric macrophage-mediated neuroinflammation plays a predominant role in the occurrence and development of ischemic stroke. Microglia undergo polarization to M1/M2-like phenotype under stress stimulation, which mediates intracellular inflammatory response. β-elemene is a natural sesquiterpene and possesses potent anti-inflammatory activity. This study aimed to investigate the anti-inflammatory efficacy and mechanism of β-elemene in ischemic stroke from the perspective of balancing microglia M1/M2-like polarization. Methods The middle cerebral artery occlusion (MCAO) model and photothrombotic stroke model were established to explore the regulation effect of β-elemene on the cerebral ischemic injury. The LPS and IFN-γ stimulated BV-2 cells were used to demonstrate the anti-inflammatory effects and potential mechanism of β-elemene regulating M1/M2-like polarization in vitro. Results In C57BL/6 J mice subjected to MCAO model and photothrombotic stroke model, β-elemene attenuated neurological deficit, reduced the infarction volume and neuroinflammation, thus improving ischemic stroke injury. β-elemene promoted the phenotype transformation of microglia from M1-like to M2-like, which prevented neurons from oxygen and glucose deprivation/reoxygenation (OGD/R) injury by inhibiting inflammatory factor release, thereby reducing neuronal apoptosis. Mechanically, β-elemene prevented the activation of TLR4/NF-κΒ and MAPK signaling pathway and increased AKT/mTOR mediated-autophagy, thereby promoting M2-like polarization of microglia. Conclusions These results indicated that β-elemene improved cerebral ischemic injury and promoted the transformation of microglia phenotype from M1-like to M2-like, at least in part, through AKT/mTOR-mediated autophagy. This study demonstrated that β-elemene might serve as a promising drug for alleviating ischemic stroke injury.

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Section: β-Elemene Prevented the Activation Of Akt/mtor Signaling Pat...mentioning

confidence: 99%

β-elemene promotes microglial M2-like polarization against ischemic stroke via AKT/mTOR signaling axis-mediated autophagy

Zhao,

Chen,

Zhang

et al. 2024

Chin Med

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Identification of glycolysis-related genes in pulpitis by bioinformatics analysis

Liang,

Wang,

Luan

et al. 2024

Preprint

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Background: Pulpitis, a common inflammation of the dental pulp, involves intricate mechanisms that are not yet fully understood. Our study aims to elucidate the alterations in genetic transcription linked to glycolysis in pulpitis and their impact on biological pathways and molecular networks. Methods: Gene expression data was collected from the GEO database. Glycolysis-related genes were identified through databases like GeneCards and MsigDB. To understand the roles of these genes, GO, KEGG pathway enrichment, and GSEA were carried out. The PPI network was constructed with STRING, and central genes were determined using cytoHubba algorithms. mRNA-miRNA and mRNA-TF regulatory interactions were obtained from TarBase, ChIPBase, and hTFtarget. We assessed differential expression of the hub genes between groups, and conducted ROC curve analysis. ssGSEA was used to examine immune cell infiltration, with pheatmap illustrating associations between hub genes and immune cells. All statistical analyses were performed using R. Results: Our analysis revealed 3480 differentially expressed genes (DEGs) in pulpitis, comprising 1591 upregulated and 1889 downregulated genes. Among these, 63 glycolysis-related differentially expressed genes (GRDEGs) were predominantly located on chromosome 11. These GRDEGs were enriched in energy metabolism processes, organelle compartments, and molecular functions, implicating key pathways in the pathology of pulpitis. PPI network analysis identified eight hub genes—HIF1A, LDHA, HK2, STAT3, TALDO1,PPARG, ALDOC, and PFKP. Additionally, ssGSEA uncovered notable differences in the infiltration levels of 28 types of immune cells between pulpitis and control samples, suggesting alterations in the immune response related to pulpitis. Conclusion: Our research offers new perspectives into the molecular mechanisms of pulpitis, particularly regarding glycolytic pathways. These results may help identify better diagnostic markers and therapeutic targets for managing pulpitis. Future studies should aim to validate these potential biomarkers and investigate their functional roles in the etiology of disease.

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Current Insights into the Roles of LncRNAs and CircRNAs in Pulpitis: A Narrative Review

Fuchen-Ramos,

Leija-Montoya,

González-Ramírez

et al. 2024

IJMS

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Pulpitis, an inflammation of the dental pulp, is generated by bacterial invasion through different ways as caries. In the establishment and development of this disease, different biological processes are involved. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are transcripts with regulatory capacity participating in different biological functions and have been implicated in different diseases. The aim of this narrative review is to critically analyze available evidence on the biological role of lncRNAs and circRNAs in pulpitis and discuss possible new research prospects. LncRNAs and circRNAs involved in pulpitis were explored, addressing their expression, molecular mechanisms, targets and biological effects studied in animal and in vitro models, as well as in studies in human patients. LncRNAs and circRNAs are emerging as key regulators of diverse biological functions in pulpitis including apoptosis, proliferation, differentiation, oxidative stress, autophagy, ferroptosis, inflammation and immune response. The molecular mechanisms performed by these non-coding RNAs (ncRNAs) involved interactions with miRNAs and the formation of regulatory networks in the context of pulpitis. Further studies more deeply analyzing the participation of lncRNAs and circRNAs in pulpitis will reveal the potential applications of these ncRNAs as biomarkers or their use in therapeutic strategies in pulp inflammation.

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Autophagy induced by hypoxia in pulpitis is mediated by HIF-1α/BNIP3

Cited by 3 publications

References 42 publications

β-elemene promotes microglial M2-like polarization against ischemic stroke via AKT/mTOR signaling axis-mediated autophagy

β-elemene promotes microglial M2-like polarization against ischemic stroke via AKT/mTOR signaling axis-mediated autophagy

Identification of glycolysis-related genes in pulpitis by bioinformatics analysis

Current Insights into the Roles of LncRNAs and CircRNAs in Pulpitis: A Narrative Review

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