Autophagy inhibition and microRNA‑199a‑5p upregulation in paclitaxel‑resistant A549/T lung cancer cells

Zeng, Tianjiao; Xu, Mingshi; Zhang, Wanli; Gu, Xiaofan; Zhao, Fangqing; Li, Xuan; Zhang, Xiongwen

doi:10.3892/or.2021.8100

Cited by 17 publications

(10 citation statements)

References 47 publications

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“…In conclusion, the miR-199a-5p/p62 axis regulates autophagy as a potential mechanism of cisplatin resistance in SCLC [ 43 ]. Zeng et al [ 79 ] showed that miR-199a-5p expression was up-regulated in paclitaxel-resistant lung cancer cell lines (A549 and H1299). The low expression of miR-199a-5p induced autophagy and made cells re-sensitive to chemotherapy drugs, while the overexpression of miR-199a-5p inhibited autophagy and desensitized cells to various chemotherapy drugs.…”

Section: Resultsmentioning

confidence: 99%

“…After all, its expression is high in some lung cancer drug-resistant cell lines. Additionally, and this is a puzzling point, miR-199a expression is up-regulated in paclitaxel-resistant lung cancer cell lines [ 79 ] and down-regulated in doxorubicin-resistant lung cancer cell lines [ 80 ]. This may be because the two different drug treatments resulted in different biological responses and the activation of signaling pathways in the lung cancer cell lines, leading to different downstream targets of miR-199a regulation.…”

Section: Discussionmentioning

confidence: 99%

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miR-199a: A Tumor Suppressor with Noncoding RNA Network and Therapeutic Candidate in Lung Cancer

Wei

Chai

et al. 2022

IJMS

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Lung cancer is the leading cause of cancer death worldwide. miR-199a, which has two mature molecules: miR-199a-3p and miR-199a-5p, plays an important biological role in the genesis and development of tumors. We collected recent research results on lung cancer and miR-199a from Google Scholar and PubMed databases. The biological functions of miR-199a in lung cancer are reviewed in detail, and its potential roles in lung cancer diagnosis and treatment are discussed. With miR-199a as the core point and a divergence outward, the interplay between miR-199a and other ncRNAs is reviewed, and a regulatory network covering various cancers is depicted, which can help us to better understand the mechanism of cancer occurrence and provide a means for developing novel therapeutic strategies. In addition, the current methods of diagnosis and treatment of lung cancer are reviewed. Finally, a conclusion was drawn: miR-199a inhibits the development of lung cancer, especially by inhibiting the proliferation, infiltration, and migration of lung cancer cells, inhibiting tumor angiogenesis, increasing the apoptosis of lung cancer cells, and affecting the drug resistance of lung cancer cells. This review aims to provide new insights into lung cancer therapy and prevention.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

miR-199a: A Tumor Suppressor with Noncoding RNA Network and Therapeutic Candidate in Lung Cancer

Wei

Chai

et al. 2022

IJMS

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“…Currently, early diagnosis and treatment of tumors have long been a hot topic in medicine and life sciences [42][43][44]. Zeng et al reported that upregulation miR-199a-5p inhibits autophagy and renders resistance to chemotherapeutic drugs in lung cancer cells by activating the PI3K/Akt/mTOR pathway and targeting p62 [45]. We will also continue to monitor the impact of the miR-199a/Rheb/mTOR axis on the development of resistance to the treatment of tumors in lung cancer, especially about EGFR-TKIs resistance.…”

Section: Discussionmentioning

confidence: 99%

miR-199a-3p/5p regulate tumorgenesis via targeting Rheb in non-small cell lung cancer

Liu¹,

Wang²,

Chai³

et al. 2022

Int. J. Biol. Sci.

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Lung cancer is one of the deadliest cancers, in which non-small cell lung cancer (NSCLC) accounting for 85% and has a low survival rate of 5 years. Dysregulation of microRNAs (miRNAs) can participate in tumor regulation and many major diseases. In this study, we found that miR-199a-3p/5p were down-expressed in NSCLC tissue samples, cell lines, and the patient sample database. MiR-199a-3p/5p overexpression could significantly suppress cell proliferation, migration ability and promote apoptosis. Through software prediction, ras homolog enriched in brain (Rheb) was identified as a common target of miR-199a-3p and miR-199a-5p, which participated in regulating mTOR signaling pathway. The same effect of inhibiting NSCLC appeared after down-regulating the expression of Rheb. Furthermore, our findings revealed that miR-199a can significantly inhibit tumor growth and metastasis in vivo, which fully demonstrates that miR-199a plays a tumor suppressive role in NSCLC. In addition, miR-199a-3p/5p has been shown to enhance the sensitivity of gefitinib to EGFR-T790M in NSCLC. Collectively, these results prove that miR-199a-3p/5p can act as cancer suppressor genes to inhibit the mTOR signaling pathway by targeting Rheb, which in turn inhibits the regulatory process of NSCLC. Thus, to investigate the anti-cancer effect of pre-miR-199a/Rheb/mTOR axis in NSCLC, have the potential to become an early diagnostic marker or therapeutic target for NSCLC.

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“…miR-342 has also been demonstrated to act as a tumor suppressor gene that inhibits the growth of colorectal carcinoma by regulating aberrant DNA hypermethylation [38]. Currently, miR-199a-5p is primarily being investigated in cancers, including epithelial ovarian cancer and lung cancer [39,40]. Pathways linked to miR-199a-5p have also been implicated in the inflammatory response to certain diseases.…”

Section: Discussionmentioning

confidence: 99%

Reconstruction and Differential Expression Profiling Core Target Analyses of the circRNA-miRNA-mRNA Network Based on Competitive Endogenous RNAs in Ulcerative Colitis

Chen

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Ulcerative colitis (UC) is a common autoimmune disease worldwide. Circular RNA (circRNA) is a type of noncoding ribonucleic acids (ncRNAs). In addition to their roles in numerous biological processes, circRNAs are also linked to a vast range of diseases including UC. Although previous studies have examined many circRNAs, the physiological and pathological roles of the circRNA-associated competing endogenous RNA (ceRNA) network in UC remain unclear. Thus, we constructed a circRNA-miRNA-mRNA network based on the ceRNA hypothesis by analyzing data from the National Center for Biotechnology Information Gene Expression Omnibus (NCBI-GEO) database. Genes with higher degree values than others in the ceRNA network were selected as central nodes when constructing the corresponding core subnetworks. To fully understand the biological function of the ceRNA network, we entered all differentially expressed mRNAs (DEmRNAs) from the ceRNA network into the Database for Annotation and Integrated Discovery (DAVID), which was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. We further entered DEmRNAs into the STRING database for protein-protein interaction (PPI) network analysis. The results elucidated that the ceRNA network comprised 403 circRNA nodes, 5 miRNA nodes, 138 mRNA nodes, and 559 edges. Three core ceRNA subnetworks centered on hsa-miR-342-3p, hsa-miR-199a-5p, and hsa-miR-142-3p were reconstructed in this study. GO and KEGG enrichment analyses identified 167 enriched GO categories and 14 enriched KEGG pathway terms. The core PPI network was composed of 15 core targets, of which CD44, HIF1A, and MMP2 were the most significant. In summary, 3 hub miRNAs (hsa-miR-342-3p, hsa-miR-199a-5p, hsa-miR-142-3p) and 3 hub genes (CD44, HIF1A, and MMP2) might play an important role in the development of UC. These hub nodes, first proposed here, might also be used as potential diagnostic markers and therapeutic targets.

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Autophagy inhibition and microRNA‑199a‑5p upregulation in paclitaxel‑resistant A549/T lung cancer cells

Cited by 17 publications

References 47 publications

miR-199a: A Tumor Suppressor with Noncoding RNA Network and Therapeutic Candidate in Lung Cancer

miR-199a: A Tumor Suppressor with Noncoding RNA Network and Therapeutic Candidate in Lung Cancer

miR-199a-3p/5p regulate tumorgenesis via targeting Rheb in non-small cell lung cancer

Reconstruction and Differential Expression Profiling Core Target Analyses of the circRNA-miRNA-mRNA Network Based on Competitive Endogenous RNAs in Ulcerative Colitis

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