Autophagy inhibition in early but not in later stages prevents 3T3-L1 differentiation: Effect on mitochondrial remodeling

Škop, Vojtěch; Cahová, Monika; Daňková, Helena; Papáčková, Zuzana; Páleníčková, Eliška; Svoboda, Petr; Zídková, J.; Kazdová, Ludmila

doi:10.1016/j.diff.2014.06.002

Cited by 34 publications

(30 citation statements)

References 20 publications

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“…In recent studies, autophagy had been identified as a regulator of cellular differentiation, suggesting that autophagy might regulate the process of adipocytic differentiation [7,8,20]. Through genetic inhibition of critical autophagy-related gene 7 (Atg7), the function of autophagy in regulating adipocytic development was examined by Singh et al and similar results were obtained by Zhang et al [7,8].…”

Section: Discussionsupporting

confidence: 64%

Strontium ranelate-induced anti-adipocytic effects are involved in negative regulation of autophagy in rat bone marrow mesenchymal stem cells

Bai

2018

International Orthopaedics (SICOT)

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Purposes Strontium ranelate (SrRN) is a novel and effective anti-osteoporotic drug used to promote bone formation and restrain bone resorption. However, studies of the effects and mechanism of SrRN on adipocytic differentiation from bone marrow mesenchymal stem cells (BMMSCs) remain limited. Methods The cytoactivity of BMMSCs was analyzed by CCK-8 and annexin V-FITC/PI kit. Then the differentiation efficiency was analyzed by Oil Red O staining and western blotting. The level of autophagy was evaluated by immunofluorescence and western blotting. The autophagy inhibitor chloroquine and the selective Akt antagonist API-2 were used to study the relationship between autophagy regulation and adipocytic differentiation. Finally variations in β-catenin protein levels were also analyzed by western blotting, as were proteins related with autophagy and differentiation signaling pathways. Results SrRN had no influence on the vatility of BMMSCs (bone marrow mesenchymal stem cells) and exhibited anti-adipocytic effects in dose-dependent manner. At the early stage of adipocytic differentiation, autophagy level significantly increased and SrRN inhibited the variation tendency. Then we detected the PPAR-γ and β-catenin variation when applied with autophagy inhibitor CQ (chloroquine) and the same way, after Akt selective inhibitor applied in the test, the effect of SrRN was compromised. At last, we detected the increased phosphorylation of Akt-related pathway at 1.00 mM concentration of SrRN in comparison with the adipocytic induction. Conclusions SrRN could disturb the process of adipocytic differentiation of BMMSCs and interfere with the variation tendency of autophagy level after adipocytic induction in Akt-dependent manners.

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Section: Discussionsupporting

confidence: 64%

Strontium ranelate-induced anti-adipocytic effects are involved in negative regulation of autophagy in rat bone marrow mesenchymal stem cells

Bai

2018

International Orthopaedics (SICOT)

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“…Skop et al [146] reported that in the first two days, mtDNA copy number declined by 50%, which was followed by a rapid increase and culminated on day 5.After that, mtDNA copy number was moderately reduced to the level observed in preadipocytes [146]. Ryu et al showed that mtDNA copy number started to increase on day 1 and peaked at day 3 during3T3-L1 adipocyte differentiation [149].…”

Section: Mitochondria and Adipocyte Differentiationmentioning

confidence: 95%

“…In preadipocytes, mitochondria are filamentous and organized as continuous reticulum. In contrast, in differentiated adipocytes, they are fragmented, punctual and redistributed around lipid droplets [146]. Mitochondrial fusion and fission have direct influence on lipid accumulation in adipocytes.…”

Section: Mitochondria and Adipocyte Differentiationmentioning

confidence: 96%

“…There is a significant "burst" of expression level of proteins involved in thetricarboxylic acid cycle(TCA) cycle, especially pyruvate carboxylase, at the very beginning of differentiation, which was followed by a more gradual and less intensive increase in the expression of genes associated with the electron transport chain, fatty acid metabolism and mitochondrial transport [146]. Above results indicate that during the transition from preadipocytes to adipocytes, cells enter a state of metabolic-overdrive characterized by simultaneous increase of flux through the TCA cycle andfatty acid oxidation [148].…”

Section: Mitochondria and Adipocyte Differentiationmentioning

confidence: 99%

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Redox modulation of adipocyte differentiation: hypothesis of “Redox Chain” and novel insights into intervention of adipogenesis and obesity

Wang

Hai

2015

Free Radical Biology and Medicine

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“…Differentiation of the mouse 3T3-L1 preadipocyte cell line was performed according to Skop et al (2014). Briefly, the cells were grown to confluence in DMEM (Sigma-Aldrich) supplemented with 10% FBS (Sigma Aldrich) in 5% CO 2 atmosphere at 37°C.…”

Section: Analysis Of M × Paradisiaca Extractsmentioning

confidence: 99%

Antidiabetic Compounds in Stem Juice from Banana

Nguyen¹,

Zachariasova²,

Spurna³

et al. 2017

Czech J. Food Sci.

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Nguyen D., Nováková A., Spurná K., Hričko J., Phung H., Viktorová J., Stránská M., Hajšlová J., Ruml T. (2017): Antidiabetic compounds in stem juice from babana grown in natural and greenhouse. Czech J. Food Sci., 35: 407-413.The stem juices from Musa × paradisiaca L. banana plants cultivated in their original natural habitat in Vietnam and those cultivated in a greenhouse in the Czech Republic were investigated for the presence of phytochemicals with antidiabetic potency. Respective bioactivities of these phytochemicals were also determined. Sample screening using ultrahigh performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPL-HRMS/MS) method showed some differences in the pattern of bioactive compounds, both in terms of their number and concentration. p-Hydroxybenzoic and gallic acids were the predominant analytes found in stem juice from plants grown in Vietnam, while ferulic acid was the major compound found in juice obtained from greenhouse bananas. Despite differences in the occurrence of potentially antidiabetic compounds, both extracts exhibited comparable inhibitory activity against α-glucosidase and α-amylase.

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Autophagy inhibition in early but not in later stages prevents 3T3-L1 differentiation: Effect on mitochondrial remodeling

Cited by 34 publications

References 20 publications

Strontium ranelate-induced anti-adipocytic effects are involved in negative regulation of autophagy in rat bone marrow mesenchymal stem cells

Strontium ranelate-induced anti-adipocytic effects are involved in negative regulation of autophagy in rat bone marrow mesenchymal stem cells

Redox modulation of adipocyte differentiation: hypothesis of “Redox Chain” and novel insights into intervention of adipogenesis and obesity

Antidiabetic Compounds in Stem Juice from Banana

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