Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions

Brasi-Velasco, Sabrina De; López-Vidal, Omar; Martí, María C.; Ortiz-Espín, Ana; Sevilla, Francisca; Jiménez, Ana

doi:10.3390/antiox10121884

Cited by 3 publications

(2 citation statements)

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“…However, it has recently been reported that overexpression of Pisum sativum mitochondrial thioredoxin (PsTRXo1) in tobacco cells (OEX) showed higher gene expression, higher protein content and higher ATG4 activity than control cells. These and further findings suggest the role of TRXs in mediating the redox regulation of autophagy in plant cells [78].…”

Section: Persulfidated Autophagy‐related Proteins In Arabidopsismentioning

confidence: 95%

Hydrogen sulfide action in the regulation of plant autophagy

Aroca

Gotor

2022

FEBS Letters

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Hydrogen sulfide is a signalling molecule with a well‐established impact on both plant and animal physiology. Intense investigation into the regulation of autophagy by sulfide in Arabidopsis thaliana has revealed that the post‐translational modification of persulfidation/S‐sulfhydration plays a key role. In this review focused on plants, we discuss the nature of the sulfide molecule involved in the regulation of autophagy, the final outcome of this modification and the persulfidated autophagy proteins identified so far. A detailed outline of the actual knowledge of the regulation mechanism of the autophagy‐related proteins ATG4a and ATG18a from Arabidopsis by sulfide is also included. This information will be instrumental for furthering research on the regulation of autophagy by sulfide.

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Section: Persulfidated Autophagy‐related Proteins In Arabidopsismentioning

confidence: 95%

Hydrogen sulfide action in the regulation of plant autophagy

Aroca

Gotor

2022

FEBS Letters

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“…Proteins were eluted from the columns, after a 5-min pre-incubation at room temperature with 20 μL of elution buffer (50 mM Tris HCl, pH 6.8, 50 mM DTT, 1% SDS, 1 mM EDTA, 0.005% bromophenol blue, 10% glycerol) preheated to 95 °C, by loading four consecutive 50 μL treatments with preheated elution buffer at 95 °C. The eluate was analysed by SDS-PAGE on AnykD gels (Bio-Rad, Spain) and Western blot as previously described [ 30 ]. Polyclonal antibody for PsTRX o 1 was used as described above.…”

Section: Methodsmentioning

confidence: 99%

Thioredoxin TRXo1 is involved in ABA perception via PYR1 redox regulation

et al. 2023

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Phytol and α-Bisabolol Synergy Induces Autophagy and Apoptosis in A549 Cells and Additional Molecular Insights through Comprehensive Proteome Analysis via Nano LC-MS/MS

Kiruthiga,

Niharika,

Devi

2024

ACAMC

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Background: Non-Small Cell Lung Cancer (NSCLC) is a malignancy with a significant prevalence and aggressive nature, posing a considerable challenge in terms of therapeutic interventions. Autophagy and apoptosis, two intricate cellular processes, are integral to NSCLC pathophysiology, each affecting the other through shared signaling pathways. Phytol (Phy) and α -bisabolol (Bis) have shown promise as potential anticancer agents individually, but their combined effects in NSCLC have not been extensively investigated. Objective: The present study was to examine the synergistic impact of Phy and Bis on NSCLC cells, particularly in the context of autophagy modulation, and to elucidate the resulting differential protein expression using LCMS/ MS analysis. Methods: The A549 cell lines were subjected to the patented effective concentration of Phy and Bis, and subsequently, the viability of the cells was evaluated utilizing the MTT assay. The present study utilized real-time PCR analysis to assess the expression levels of crucial apoptotic genes, specifically Bcl-2, Bax, and Caspase-9, as well as autophagy-related genes, including Beclin-1, SQSTM1, Ulk1, and LC3B. The confirmation of autophagy marker expression (Beclin-1, LC3B) and the autophagy-regulating protein SQSTM1 was achieved through the utilization of Western blot analysis. Differentially expressed proteins were found using LC-MS/MS analysis. Results: The combination of Phy and Bis demonstrated significant inhibition of NSCLC cell growth, indicating their synergistic effect. Real-time PCR analysis revealed a shift towards apoptosis, with downregulation of Bcl-2 and upregulation of Bax and Caspase-9, suggesting a shift towards apoptosis. Genes associated with autophagy regulation, including Beclin-1, SQSTM1 (p62), Ulk1, and LC3B, showed significant upregulation, indicating potential induction of autophagy. Western blot analysis confirmed increased expression of autophagy markers, such as Beclin-1 and LC3B, while the autophagy-regulating protein SQSTM1 exhibited a significant decrease. LC-MS/MS analysis revealed differential expression of 861 proteins, reflecting the modulation of cellular processes. Protein-protein interaction network analysis highlighted key proteins involved in apoptotic and autophagic pathways, including STOML2, YWHAB, POX2, B2M, CDA, CAPN2, TXN, ECHS1, PEBP1, PFN1, CDC42, TUBB1, HSPB1, PXN, FGF2, and BAG3, emphasizing their crucial roles. Additionally, PANTHER pathway analysis uncovered enriched pathways associated with the differentially expressed proteins, revealing their involvement in a diverse range of biological processes, encompassing cell signaling, metabolism, and cellular stress responses. Conclusion: The combined treatment of Phy and Bis exerts a synergistic inhibitory effect on NSCLC cell growth, mediated through the interplay of apoptosis and autophagy. The differential protein expression observed, along with the identified proteins and enriched pathways, provides valuable insights into the underlying molecular mechanisms. These findings offer a foundation for further exploration of the therapeutic potential of Phy and Bis in the management of NSCLC.

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Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions

Cited by 3 publications

References 72 publications

Hydrogen sulfide action in the regulation of plant autophagy

Hydrogen sulfide action in the regulation of plant autophagy

Thioredoxin TRXo1 is involved in ABA perception via PYR1 redox regulation

Phytol and α-Bisabolol Synergy Induces Autophagy and Apoptosis in A549 Cells and Additional Molecular Insights through Comprehensive Proteome Analysis via Nano LC-MS/MS

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