Autophagy‐related 5 gene mRNA expression and ATG5 rs510432 polymorphism in children with bronchial asthma

Ahmad, Enas S.; Diab, Safia M; Behiry, Eman G.; Bassyoni, Shereen El Bassyoni El Said El; Ishak, Sally Raafat; Ramadan, Abeer

doi:10.1002/ppul.26079

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…SNPs are the most common genetic variations in a single nucleotide of the DNA and are also popularly found in noncoding segments. For instance, polymorphisms in the noncoding segments of ATG5, rs510432 and rs506027, are linked to susceptibility to bronchial asthma, epilepsy, and sepsis 42–44 . Recently, some studies have found that gene mutations in the coding region also affect the occurrence of various diseases.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, polymorphisms in the noncoding segments of ATG5, rs510432 and rs506027, are linked to susceptibility to bronchial asthma, epilepsy, and sepsis. [42][43][44] Recently, some studies have found that gene mutations in the coding region also affect the occurrence of various diseases. rs2245214 and rs573775 in the coding segments of ATG5 were associated with susceptibility to chronic Q fever, non-small cell lung cancer, and systemic lupus erythematosus.…”

Section: Discussionmentioning

confidence: 99%

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Association of ATG16L1 and ATG5 gene polymorphisms with susceptibility to hepatitis B virus infection and progression to HCC in central China

Wu,

Ouyang

2023

Microbiology and Immunology

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Hepatitis B virus (HBV) infection is a severe public health problem worldwide. The relationship between polymorphisms of autophagy‐related 16‐like 1 gene (ATG16L1) and autophagy‐related gene 5 (ATG5) with susceptibility to the stage of HBV infection has been reported in different populations. Nevertheless, this association is not seen in the population of central China. This study recruited 452 participants, including 246 HBV‐infected patients (139 chronically infected HBV without hepatocellular carcinoma [HCC] and 107 HBV‐related HCC patients) and 206 healthy controls. Genotyping of ATG16L1 rs2241880 and ATG5 rs688810 were performed using Sanger sequencing and polymerase chain reaction‐restriction fragment length polymorphism, respectively. Our results indicated that the G allele of ATG16L1 rs2241880 was more frequent in healthy controls than in patients with chronicHBV infection. After adjusting for age and sex, an association between the ATG16L1 rs2241880 polymorphism and HBV infection was significant under the dominant and allele models (p = 0.009 and 0.003, respectively). However, no association between the ATG5 polymorphisms and HBV infection was observed. We also did not find a significant association between ATG16L1 and ATG5 polymorphisms and the progression of HBV‐related HCC. Therefore, the genetic polymorphism of ATG16L1 rs2241880 may be associated with susceptibility to HBV infection in the population of central China.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association of ATG16L1 and ATG5 gene polymorphisms with susceptibility to hepatitis B virus infection and progression to HCC in central China

Wu,

Ouyang

2023

Microbiology and Immunology

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“…ATG5, as one of the essential proteins regulating autophagy, is considered to overexpress and mediate immune responses in asthma [ 12 , 15 , 20 ]. Previous reports suggest that compared with HCs, ATG5 is highly expressed in asthma children [ 13 , 15 ]. However, no study has explored ATG5 level and its relationship with clinical features in adult asthma patients.…”

Section: Discussionmentioning

confidence: 99%

“…The above studies reveal the potential of ATG5 as a marker for asthma management. However, the previous studies have only shown an abnormal expression of ATG5 in asthma children [ 13 , 15 ]. Moreover, the clinical roles of ATG5 in adult asthma patients have not been explored.…”

Section: Introductionmentioning

confidence: 99%

Serum autophagy protein 5 is positively related to T helper 2/T helper 1 ratio, inflammation, and exacerbation in adult asthma patients

Ke,

Xie

2023

Allergy Asthma Clin Immunol

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Background Autophagy protein 5 (ATG5) regulates airway epithelial cell autophagy, immune response, and inflammation, which is involved in asthma progression. This study aimed to evaluate ATG5 levels and its clinical roles in adult asthma patients. Methods Totally, 200 adult asthma patients and 100 healthy controls (HCs) were enrolled in this case-control study. Subsequently, serum ATG5 was measured by enzyme-linked immunosorbent assay. Results ATG5 was increased in asthma patients compared with HCs [median (interquartile range): 44.2 (31.7–77.8) vs. 23.2 (16.7–39.2) ng/mL] (P < 0.001). In asthma patients, ATG5 was positively related to male gender (P = 0.022), a family history of asthma (P = 0.035), eosinophil count (P < 0.001), and immune globulin E (P < 0.001), while it was negatively correlated with forced expiratory volume in 1 s (FEV1)/forced vital capacity (P < 0.001) and FEV1 (Predicted) (P < 0.001). Meanwhile, ATG5 was inversely associated with T helper (Th) 1 cells (P = 0.008), while it was positively linked with Th2 cells (P < 0.001), Th2/Th1 ratio (P < 0.001), interleukin (IL)-4 (P = 0.002), and IL-4/interferon-γ ratio (P = 0.015). Additionally, ATG5 was positively correlated with tumor necrosis factor-α (P < 0.001), IL-1β (P = 0.001), IL-6 (P = 0.003), and IL-17 (P = 0.029). Notably, ATG5 was elevated in asthma patients at exacerbation compared to those at remission [median (interquartile range): 53.6 (37.6–90.0) vs. 35.6 (28.2–51.5) ng/mL] (P < 0.001). It was also noteworthy that ATG5 was positively linked with exacerbation severity in asthma patients (P = 0.005). Conclusion Serum ATG5 is related to increased Th2/Th1 ratio, inflammation, exacerbation risk and severity in adult asthma patients, which serves as a candidate marker for the management of asthma. However, further validation is still needed.

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“…[ 4 ] Autophagy has been associated with inflammatory diseases, cardiovascular diseases, neurodegenerative diseases, and cancers. [ 5 ] Ahmad et al [ 6 ] highlighted the pivotal role of ARG ATG5 in pediatric asthma. Furthermore, microRNA (miRNA, miR)-30a was identified to target ATG5, thereby inhibiting autophagy, which subsequently leads to a reduction in airway fibrosis in patients with asthma.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics analysis of ceRNA network of autophagy-related genes in pediatric asthma

Zhu,

Shi,

2023

Medicine

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The molecular underpinnings of pediatric asthma present avenues for targeted therapies. A deeper exploration into the significance of differentially expressed autophagy-related genes (DE-ARGs) and their interactions with the long noncoding RNA (lncRNA)–microRNA (miRNA)–mRNA network may offer insights into the pathogenesis of pediatric asthma. DE-ARGs were retrieved from the Gene Expression Omnibus and the Human Autophagy Database. These DE-ARGs were subjected to comprehensive analyses, including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway, Gene Set Enrichment Analysis, and protein–protein interaction networks. The identified DE-ARGs were further verified for core gene expression. The miRDB and ENCORI databases were used for inverse miRNA predictions. Furthermore, miRNA–lncRNA interactions were predicted using LncBase and ENCORI platforms. Following the exclusion of lncRNAs exclusively localized in the nucleus and extracellular space, a competitive endogenous RNA (ceRNA) network was established and subsequently subjected to detailed analysis. The mRNA expression patterns in the ceRNA network were validated using quantitative real-time PCR. In total, 31 DE-ARGs were obtained, of which 29 were up-regulated and 2 were down-regulated. Notably, the autophagy, regulation of apoptotic signaling pathways, interferon-α/β signaling, interferon γ signaling, autophagy-animal, and apoptosis pathways were predominantly enriched in pediatric asthma. Five hub genes (VEGFA, CFLAR, RELA, FAS, and ATF6) were further analyzed using the Gene Expression Omnibus dataset to verify their expression patterns and diagnostic efficacy. Four hub genes (VEGFA, CFLAR, RELA, and FAS) were obtained. Finally, a ceRNA network of 4 mRNAs (VEGFA, CFLAR, RELA, and FAS), 3 miRNAs (hsa-miR-320b, hsa-miR-22-3p, and hsa-miR-625-5p), and 35 lncRNAs was constructed by integrating data from literature review and analyzing the predicted miRNAs and lncRNAs. Moreover, the quantitative real-time PCR data revealed a pronounced upregulation of Fas cell surface death receptor. The identification of 4 DE-ARGs, especially Fas cell surface death receptor, has shed light on their potential pivotal role in the pathogenesis of pediatric asthma. The established ceRNA network provides novel insights into the autophagy mechanism in asthma and suggests promising avenues for the development of potential therapeutic strategies.

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Autophagy‐related 5 gene mRNA expression and ATG5 rs510432 polymorphism in children with bronchial asthma

Cited by 11 publications

References 23 publications

Association of ATG16L1 and ATG5 gene polymorphisms with susceptibility to hepatitis B virus infection and progression to HCC in central China

Association of ATG16L1 and ATG5 gene polymorphisms with susceptibility to hepatitis B virus infection and progression to HCC in central China

Serum autophagy protein 5 is positively related to T helper 2/T helper 1 ratio, inflammation, and exacerbation in adult asthma patients

Bioinformatics analysis of ceRNA network of autophagy-related genes in pediatric asthma

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