Autoradiographic detection of3H-fucose incorporation into glycoprotein by odontoblasts and its deposition at the site of the calcification front in dentin

Weinstock, A.; Weinstock, Melvyn; Leblond, C. P.

doi:10.1007/bf02010136

Cited by 84 publications

(30 citation statements)

References 18 publications

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“…The appearance of silver grains over the mineralization front of dentin at 4 h in both the control and LACA-treated animals may represent noncollagenous matrix glycoproteins of odontoblast origin as previously reported by Weinstock et al [32]. If the silver grains do in fact represent 3H-glycine labeled glycoprotein of odontoblast origin, one would have to conclude that such glycoproteins are secreted by some other route than the typical collagen-containing secretion granule.…”

Section: Discussionmentioning

confidence: 66%

Comparative radioautographic study of the effects of L-azetidine-2-carboxylic acid on matrix secretion and golgi of the mouse incisor

Cho

Garant

1984

Calcif Tissue Int

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The effect of a proline analog, L-azetidine-2-carboxylic acid (LACA), on protein matrix secretion by odontoblasts and ameloblasts was compared by light and electron microscopic radioautography after injection of 3H-glycine in young mice. LACA inhibited the secretion of dentin matrix with consequent accumulation of 3H-glycine labeled procollagen in the cisternae of the rough endoplasmic reticulum. In contrast, LACA had no apparent effect on ameloblasts as enamel matrix continued to be packaged in the Golgi apparatus and secreted from Tomes' process within 30 min after injection of the radioprecursor. Electron microscopy revealed that LACA did not cause any change in ameloblast ultrastructure but produced a marked alteration of the odontoblast Golgi complex. All odontoblast Golgi saccules and collagen secretion granules disappeared within 2 h after LACA administration. Odontoblast Golgi cisternae, however, appeared not to be affected. These observations confirm previous studies conducted in this laboratory showing that Golgi saccules in collagen-secreting cells are the initial staging areas for the formation of secretory granules. These results also indicate that a close correlation exists between form and function in the Golgi apparatus of collagen-secreting cells.

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Section: Discussionmentioning

confidence: 66%

Comparative radioautographic study of the effects of L-azetidine-2-carboxylic acid on matrix secretion and golgi of the mouse incisor

Cho

Garant

1984

Calcif Tissue Int

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“…All four antibodies yield essentially the same labeling pattern. Consistent with other studies (Riminucci et al 1995;Bosshardt et al 1998;Nanci 1999), immunolabeling for BSP and OPN is associated with the electron-dense regions of the extracellular matrix, viz., regions that are indicative of mineralized matrix and correspond to the dark matrix regions in decalcified sections that are stained by metachromatic dyes for light microscopy (Weinstock et al 1972;Schroeder 1991, 1994;McKee and Nanci 1993). The present study shows, for the first time, that the region of the established interface between the resorbed dentin and the repair cementum in human deciduous teeth is heavily labeled for both BSP and OPN.…”

Section: Discussionmentioning

confidence: 99%

Sequence of protein expression of bone sialoprotein and osteopontin at the developing interface between repair cementum and dentin in human deciduous teeth

2005

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Experimental periodontal regeneration studies have revealed the weak binding of repair cementum to the root surface, whereas attachment of cementum to dentin preconditioned by odontoclasts appears to be superior. The aim of this study has been, therefore, to analyze the structural and partial biochemical nature of the interface that develops between resorbed dentin and repair cementum by using human deciduous teeth as a model. Aldehyde-fixed and decalcified tooth samples were embedded in acrylic or epoxy resins and sectioned for light and transmission electron microscopy. Antibodies against bone sialoprotein (BSP) and osteopontin (OPN), two noncollagenous proteins accumulating at hard tissue interfaces in bone and teeth, were used for protein A-gold immunocytochemistry. Light microscopy revealed a gradually increasing staining intensity of the external dentin matrix starting after the withdrawal of the odontoclast. Labeling for both BSP and OPN was first detected among the exposed collagen fibrils and in the intratubular dentin matrix when odontoclasts had withdrawn but mesenchymal cells were present. Subsequently, collagen fibrils of the repair cementum were deposited concomitantly with the appearance of labeling for BSP and OPN over the intratubular, intertubular, and peritubular dentin matrix. Labeled mineralization foci indicated the advancing mineralization front, and the collagenous repair matrix became integrated in an electron-dense organic material that showed labeling for BSP and OPN. Thus, no distinct planar interfacial matrix layer lies between the resorbed dentin and the repair cementum. The results suggest that odontoclasts precondition the dentin matrix such that the repair cementum becomes firmly attached.

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“…Autoradiography, cytochemistry, immunocytochemistry, and elemental x-ray analysis have been used to elucidate various aspects of odontoblastic secretory activity (Katchburian and Holt, 1968;Weinstock et al, 1972;Weinstock and Leblond, 1974;Karim et al, 1979;Frank and Nalbandian, 1989). In addition to matrix deposition, the odontoblast participates in the removal of organic matrix breakdown products, presumably generated during the conversion of nonmineralized predentin to mineralized dentin.…”

mentioning

confidence: 99%

Structure and organization of odontoblasts

Sasaki

Garant

1996

Anat. Rec.

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Autoradiographic detection of3H-fucose incorporation into glycoprotein by odontoblasts and its deposition at the site of the calcification front in dentin

Cited by 84 publications

References 18 publications

Comparative radioautographic study of the effects of L-azetidine-2-carboxylic acid on matrix secretion and golgi of the mouse incisor

Comparative radioautographic study of the effects of L-azetidine-2-carboxylic acid on matrix secretion and golgi of the mouse incisor

Sequence of protein expression of bone sialoprotein and osteopontin at the developing interface between repair cementum and dentin in human deciduous teeth

Structure and organization of odontoblasts

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