Autoradiographic determination of angiotensin II receptors in prepubertal and postpubertal pig ovarian tissue

Shuttleworth, Gail; Hunter, M. G.; Pipkin, Fiona Broughton

doi:10.1530/rep.0.1220701

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…44,45 In the ovary, AT1 and AT2 receptors have been characterized in different species including rat, [46][47][48][49] bovine, 50 and porcine. [51][52][53] In general, the wide interspecies variation in relation to the expression and cellular localization of AngII receptors in ovarian follicles is suggestive of differences among species in the physiological roles of OVRAS. In the rat, AT2 receptor has been linked to follicular atresia 54 and apoptosis of granulosa lutein (GL) cells.…”

Section: Ras Receptors and Signal Transduction Systems Involved In Ovras Effectsmentioning

confidence: 99%

The Ovarian Renin-Angiotensin System (OVRAS): A Major Factor in Ovarian Function and Disease

2016

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This contribution summarizes the pivotal role of the ovarian renin-angiotensin system (OVRAS) in ovarian physiology and disease, with particular emphasis on human clinical implications and established translational applications. The presence of a complete OVRAS in all studied species has been known for decades. The OVRAS has major effects on follicle development/atresia and ovulation and steroid hormone secretion, that is, it is necessary for normal reproduction. It is well established that OVRAS activity is regulated by gonadotropins and depends on activation of proteases in the area of growing follicles. Angiotensin and angiotensin receptors are widely distributed in the ovarian follicle, preovulatory theca and granulosa cells, and postovulatory mural granulosa-lutein cells and regulate steroidogenesis. Molecular blockade of the OVRAS inhibits oocyte maturation and ovulation. Pathologically abnormal OVRAS function has been associated with infertility, polycystic ovarian syndrome (PCOS), ovarian hyperstimulation syndrome (OHSS), and ovarian cancer. Both hyperandrogenism in PCOS and third space fluid accumulation in OHSS have been convincingly linked to overexpression of renin and angiotensin. Blockade of angiotensin receptors is under study for the treatment of gynecologic cancer, OHSS, and PCOS. However, a full understanding of the OVRAS and translational applications is lacking. In part, this is due to the discovery in recent years of previously unknown renin-angiotensin system (RAS) components and novel functions of "classical" RAS components that remain to be integrated into translational studies; newer, more specific agents to block RAS components are available only now for such research and treatment. The need for further studies is evident.

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Section: Ras Receptors and Signal Transduction Systems Involved In Ovras Effectsmentioning

confidence: 99%

The Ovarian Renin-Angiotensin System (OVRAS): A Major Factor in Ovarian Function and Disease

2016

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“…1995b). An autoradiographical study in our laboratory has indicated that there are both AT 1 and AT 2 receptors within the pre‐ and postpubertal porcine ovary, although the methodology did not allow the precise localization of these receptors (Shuttleworth et al. 2001).…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical localization of angiotensin II receptor subtypes 1 and 2 in the porcine fetal, prepubertal and postpubertal ovary

et al. 2002

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There is considerable evidence for a mammalian ovarian renin-angiotensin system, which may influence ovulation, angiogenesis and steroidogenesis via the autocrine and/or paracrine actions of the biologically active product of the cascade, angiotensin II (AngII). There are two characterized AngII receptors -type 1, AT 1 and type 2, AT 2 .We report the localization of these receptor subtypes within porcine fetal, prepubertal and postpubertal ovaries. Positive staining for AT 1 and AT 2 receptors was observed in egg nests in all fetal ovaries studied, as well as in a defined two-cell layer at the ovarian periphery. In prepubertal tissue, positive AT 1 and AT 2 staining was localized to granulosa cells adjacent to the basement membrane of pre-antral and antral follicles, with no staining in the thecal layer. There was immunostaining for both receptors in prepubertal oocytes and zona pellucida. In postpubertal tissue, positive AT 1 and AT 2 immunostaining was localized to areas of putative neovascularization, the zona pellucida and the oocyte. Further AT 1 staining was located to the postpubertal antral follicle granulosa cells. The results indicate that there are higher densities of AT 1 receptors than AT 2 receptors in the porcine fetal, prepubertal and postpubertal ovary, and this has profound implications for the role of AngII in ovarian development.

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The ontogeny of components of the renin–angiotensin system in the porcine fetal ovary

Pountain

Pipkin

Hunter

2010

Animal Reproduction Science

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Autoradiographic determination of angiotensin II receptors in prepubertal and postpubertal pig ovarian tissue

Cited by 3 publications

References 18 publications

The Ovarian Renin-Angiotensin System (OVRAS): A Major Factor in Ovarian Function and Disease

The Ovarian Renin-Angiotensin System (OVRAS): A Major Factor in Ovarian Function and Disease

Immunocytochemical localization of angiotensin II receptor subtypes 1 and 2 in the porcine fetal, prepubertal and postpubertal ovary

The ontogeny of components of the renin–angiotensin system in the porcine fetal ovary

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