Autoradiographic localizatio of [125I]-C-ANP compared to [125I]-ANP in rat tissue

Heidemann, Franziska; Steinke, Wolfram; Pleiß, U.; Theiss, G.; Voges, K.-P.; Gerzer, R.; Stasch, Johannes‐Peter

doi:10.1007/bf00271352

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…It is thus uncertain which of the receptor subtypes, ANF-R, or ANF-R= or both, are responsible for the inhibition of NE release . Binding sites for NPs have been identified in adrenomedullary tissue of several species (Fuchs et al ., 1986;Heidemann et al ., 1991) . PC 12 cells appear to express both ANF-R , and ANF-R= receptor subtypes (Rathinavelu and Isom, 1991) .…”

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confidence: 99%

Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R₂ Receptors

Babinski

Haddad²,

Vallerand³

et al. 1995

Journal of Neurochemistry

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There is increasing evidence that members of the natriuretic peptide family display sympathoinhibitory activity, but it remains uncertain which receptor pathway is implicated. We performed cyclic GMP production studies with chromaffin cells treated with either atrial natriuretic factor (ANF) or C‐type natriuretic peptide (CNP) and found that these cells specifically express the ANF‐R1C but not the ANF‐R1A receptor subtype. Evidence for the existence of ANF‐R2 receptors was obtained from patch‐clamp experiments where C‐ANF, an ANF‐R2‐specific agonist, inhibited nicotinic currents in single isolated chromaffin cells. Involvement of ANF‐R2 receptors in the modulation of nicotinic currents was further supported by the significant loss of this inhibitory activity after the cleavage of the disulfide‐bridged structure of C‐ANF. This linearized form of C‐ANF also displayed a lower binding affinity for ANF‐R2 receptors. Like the patch‐clamp studies, secretion experiments demonstrated that both CNP and C‐ANF are equally effective in reducing nicotine‐evoked catecholamine secretion by cultured chromaffin cells, raising the possibility that this effect of CNP is predominantly mediated by the ANF‐R2 and not the ANF‐R1C receptors. Finally, this response appears to be specific to nicotinic agonists because neither histamine‐ nor KCI‐induced secretions were affected by natriuretic peptides. In the present study, we report (1) the presence of ANF‐R1C and ANF‐R2 receptor subtypes in bovine chromaffin cells, (2) the inhibition by natriuretic peptides of nicotinic whole‐cell currents as well as nicotine‐induced catecholamine secretion, (3) the possible mediation of these effects by the ANF‐R2 class of receptors, and (4) the specificity of this inhibition to nicotinic agonists. Because bovine chromaffin cells release ANF, BNP, and CNP together with catecholamines, all three peptides might exert negative feedback regulation of catecholamine secretion in an autocrine manner by interacting with the nondiscriminating ANF‐R2 receptor subtype.

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Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R₂ Receptors

Babinski

Haddad²,

Vallerand³

et al. 1995

Journal of Neurochemistry

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“…Infusions of ANP-C receptor ligands like C-ANP(4-23), SC 46542, or C-ANP (11)(12)(13)(14)(15) increase natriuresis in intact rats via an increase in endogenous plasma ~~~( 8 , 1 1 -1 3 ) . ANP-A and ANP-B receptors are coupled to the membrane-bound guanylate cyclase and mediate the effects of the natriuretic peptides (ANP, BNP, CNP and urodilatin)(5-7).…”

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confidence: 99%

Interaction of a Neutral Endopeptidase Inhibitor with an ANP-C Receptor Ligand in Anesthetized Dogs

Wegner

Stasch

Hirth-Dietrich

et al. 1995

Clinical and Experimental Hypertension

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Inhibition of important degradative pathways of atrial natriuretic peptide (ANP) in vivo could be a valuable therapeutic tool for regulating endogenous levels of ANP. The aim was to investigate the in vivo effects of both blockade of atrial natriuretic peptide clearance receptor and inhibition of neutral endopeptidase 24.11, an enzyme shown to be involved in ANP breakdown. Therefore, we infused a specific neutral endopeptidase inhibitor ((S)-thiorphan) and an ANP-C receptor ligand (AP 811) alone or in combination into anaesthetized beagle dogs. Compared with vehicle controls, coadministration of (S)-thiorphan and AP 811 (100 micrograms/kg/min and 10 micrograms/kg/min, resp.) had greater effects on endocrine and renal parameters than administration of either substance alone. Coadministration of both compounds increased urinary excretion of volume and sodium, cGMP and ANP. We found also increased plasma cGMP, plasma ANP and decreased plasma renin activity. No effects were observed with respect to blood pressure, left ventricular pressure or heart rate during the infusion period of 2 h. We conclude from these investigations, that blocking both degrading pathways of ANP with the ANP-C receptor ligand AP 811 and the neutral endopeptidase inhibitor (S)-thiorphan is more effective than inhibition of either system alone. Such a combination might therefore be a useful therapeutic tool in cardiovascular diseases.

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The three subtypes of atrial natriuretic peptide (ANP) receptors are expressed in the rat adrenal gland

Grandclément¹,

Ronsin²,

Morel³

1997

Biology of the Cell

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Atrial natriuretic peptide (ANP) actions are mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANP receptor-A (or GC-A), -B (or GC-B) and -C (the so-called clearance receptor). In rat adrenal gland, the mRNA for each subtype was detected using 35S-dUTP or digoxigenin-11-dUTP specific labeled probes, and in situ hybridization at light and electron microscopic levels respectively. The three subtypes were expressed the most abundantly in the zona glomerulosa. The amount of GC-A mRNA expression, quantified using macro-autoradiography and densitometry, was higher than the amounts of GC-B mRNA and ANPR-C mRNA both in zona glomerulosa and medullary of adrenal gland. At electron microscopic level, the three subtypes of ANPR were revealed in glomerulosa cells. A noticeable signal was also present in the medullary area, especially for GC-A mRNA, in adrenaline-containing chromaffin cells. No signal was detected in noradrenaline-containing chromaffin cells. The subcellular localization of the three mRNAs is similar: in the cytoplasmic matrix and in the euchromatin of the nucleus in each cell of glomerulosa, and in the same compartments of the adrenaline-containing chromaffin cells. These data indicate that the adrenal gland is an important target tissue for ANP action both in glomerulosa cells and adrenaline-containing chromaffin cells. The mRNA expression levels were different for each ANPR subtype.

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Autoradiographic localizatio of [125I]-C-ANP compared to [125I]-ANP in rat tissue

Cited by 4 publications

References 27 publications

Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R₂ Receptors

Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R₂ Receptors

Interaction of a Neutral Endopeptidase Inhibitor with an ANP-C Receptor Ligand in Anesthetized Dogs

The three subtypes of atrial natriuretic peptide (ANP) receptors are expressed in the rat adrenal gland

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Autoradiographic localizatio of [125I]-C-ANP compared to [125I]-ANP in rat tissue

Cited by 4 publications

References 27 publications

Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R2 Receptors

Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R2 Receptors

Interaction of a Neutral Endopeptidase Inhibitor with an ANP-C Receptor Ligand in Anesthetized Dogs

The three subtypes of atrial natriuretic peptide (ANP) receptors are expressed in the rat adrenal gland

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Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R₂ Receptors

Natriuretic Peptides Inhibit Nicotine‐Induced Whole‐Cell Currents and Catecholamine Secretion in Bovine Chromaffin Cells: Evidence for the Involvement of the Atrial Natriuretic Factor R₂ Receptors