Autoradiographic localization of atriopeptin III receptors in rat kidney

Murphy, Kenneth M.; McLaughlin, Laura L.; Michener, Marshall L.; Needleman, Philip

doi:10.1016/0014-2999(85)90772-1

Cited by 45 publications

(4 citation statements)

References 3 publications

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“…Sub sequently, we demonstrated by in vitro autoradiography that the specific binding sites for a-rAN P (28 amino acid residues) were localized on the glomeruli, renal artery, renal pelvis, and inner medullary tissues including the collecting tubule (2). More recently, some of our initial observations were confirmed in brief reports of other in vestigators using the similar technique (3,4).…”

supporting

confidence: 72%

Intrarenal Localization of Receptors for α-Rat Atrial Natriuretic Polypeptide: An Autoradiographic Study with [125I]-Labeled Ligand Injected in Vivo into the Rat Aorta

Koseki

Kanai

Hayashi

et al. 1986

Japanese Journal of Pharmacology

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Abstract-We examined intrarenal localization of receptors for a-rat atrial natriuretic polypeptide (a-rANP) by injecting [1251]-labeled ligand in vivo into the rat aorta. We found that the receptors for a-rANP are distributed also on the vasa recta of the outer and inner medulla in addition to the previously reported sites, i.e., the renal arteries, renal pelvis, glomeruli, and inner medullary tissues including collecting tubules.In the vascular bundle of the outer medulla, the majority of grains was preferentially localized on the arterial vasa recta. The electron microscopic auto radiography of the glomerulus showed that the binding sites were mainly localized on the foot process of the podocyte.Since a-rANP injected into the aorta under physiological conditions was bound to the glomerulus and vasa recta in the kidney, the effect of ANP on these binding sites may be important in the mechanism of natriuresis.

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supporting

confidence: 72%

Intrarenal Localization of Receptors for α-Rat Atrial Natriuretic Polypeptide: An Autoradiographic Study with [125I]-Labeled Ligand Injected in Vivo into the Rat Aorta

Koseki

Kanai

Hayashi

et al. 1986

Japanese Journal of Pharmacology

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“…Our laboratory was one of the first to identify ANF receptors on renal membranes, but we did not localize these receptors to a specific site in the kidney. 15 Later, there was abundant evidence of localization of ANF receptors on the glomeruli 16 but binding to renal tubules was scant. Quite recently several papers have appeared documenting a renal tubular receptor that is linked through guanylate cyclase and mediates sodium transport.…”

Section: -13mentioning

confidence: 99%

Atrial natriuretic factor plays a significant role in body fluid homeostasis.

Blaine

1990

Hypertension

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Atrial natriuretic factor (ANF) is a hormone with the physiological characteristics of a regulator of body fluid volume. It is potent, has a short duration of action, and responds to a physiologically relevant stimulus in a negative feedback-controlled system. It can act directly or indirectly (via inhibition of aldosterone biosynthesis) on the kidney to alter sodium transport and may regulate fluid distribution within the extracellular space. The peptide circulates at low (nanomolar) levels, and recent studies with renal inner medullary cells document relevant receptor binding and second messenger activation in this concentration range. In vivo data support a direct action on the kidney to enhance natriuresis, and blockade of a primary catabolic pathway for ANF within the kidney results in augmented natriuresis at concurrent endogenous peptide concentrations. Long-term, low dose infusion directly into the renal artery of conscious dogs supports a physiological action of ANF to promote urinary sodium excretion. Nevertheless, under certain circumstances, natriuresis does not occur even at high circulating levels of ANF. Apparently other factors such as renal perfusion pressure, volume status, and renal nerve activity are important in determining the natriuretic response to a given level of peptide. We hypothesize that the role played by ANF in volume regulation is highly complex, and the kidney responds with increased sodium excretion only when a constellation of variables is appropriately arrayed. That is, ANF is a necessary, but not sufficient, condition to induce natriuresis. {Hypertension 1990;15:2-8) W hen the first cell wrapped itself in a membrane, volume regulation became a key to survival. The factors controlling body fluid volume (e.g., the balance between intake and output of salt and water) are many and complex. Intake is mediated through thirst and salt appetite, whereas output is a complex interplay of variables that primarily affect renal function. Historically, the factors considered important for regulation of the renal output of salt and water are glomerular filtration and the facilitated reabsorption of sodium by aldosterone.The actions of aldosterone in regulating sodium reabsorption from the nephron and changes in glomerular filtration have not proven adequate to explain all of the experimental findings related to extracellular volume control. Based on the compelling experiments of DeWardener et al, 1 in which it was shown that the natriuresis associated with saline volume expansion was not prevented by controlling filtration and aldosterone levels, the concept of a salt-excreting reflex pathway was established. Presumably, there

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“…Previous reports have indicated that ANP receptors from a variety of tissues possess different affinities for the circulating form of the hormone [ANP(99-126)] and its analogs (Hirata et al, 1984;Ballermann et al, 1985;De Lean et al, 1985;Hirose et al, 1985;Misono et al, 1985;Murphy et al, 1985;Schenk et al, 1987). It has also been shown that in several tissues the Kd for ANP, as measured in binding assays, can differ from the EC,, for guanylate cyclase stimulation by as much as loo-fold (Hirata et al, 1984;Leitman and Murad, 1986;Neuser and Bellemann, 1986).…”

Section: Discussionmentioning

confidence: 99%

Characterization of solubilized atrial natriuretic peptide receptors from rat olfactory bulb and A10 cultured smooth muscle cells

1988

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Atrial natriuretic peptide (ANP) receptors from A10 cultured vascular smooth muscle cells (VSMC) and rat olfactory bulbs have been solubilized and then pharmacologically and biochemically compared. The dissociation constant for 125I-ANP(99-126) was 12.7 pM for the VSMC-derived receptor and 164 pM for the olfactory receptor. Competition binding between 125I-ANP(99-126) and several unlabeled ANP analogs with the soluble olfactory receptor, demonstrated a rank order potency of ANP(99-126) = ANP(103-126) much greater than ANP(103-123). However, the rank order potency of the soluble VSMC ANP receptor was ANP(99-126) = ANP(103-126) = ANP(103-123). Therefore, the olfactory ANP receptor appears to require the complete COOH-terminal sequence of ANP as compared with the VSMC ANP receptor. When the 2 soluble receptor preparations were applied to a GTP-agarose column, a portion of the olfactory ANP receptor was retained on the column and could be eluted with 5 mM GTP, while the VSMC ANP receptor did not adsorb to the column. Since the olfactory bulb ANP receptor has been shown to contain a binding component of 116 kDa, while the VSMC ANP receptor binding component is 66 kDa, these receptors appear to be similar to the 2 receptor classes described recently in which the 120 kDa receptor that binds GTP is postulated to be coupled to guanylate cyclase, while the 60 kDa receptor does not bind GTP, is not coupled to guanylate cyclase, and may possess a hormone clearance function. Taken together, these data indicate that cyclic GMP appears to be a second messenger for ANP in the brain.

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Autoradiographic localization of atriopeptin III receptors in rat kidney

Cited by 45 publications

References 3 publications

Intrarenal Localization of Receptors for α-Rat Atrial Natriuretic Polypeptide: An Autoradiographic Study with [125I]-Labeled Ligand Injected in Vivo into the Rat Aorta

Intrarenal Localization of Receptors for α-Rat Atrial Natriuretic Polypeptide: An Autoradiographic Study with [125I]-Labeled Ligand Injected in Vivo into the Rat Aorta

Atrial natriuretic factor plays a significant role in body fluid homeostasis.

Characterization of solubilized atrial natriuretic peptide receptors from rat olfactory bulb and A10 cultured smooth muscle cells

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