Autoradiographic localization of substance P ligand binding sites and distribution of immunoreactive neurons in the periaqueductal gray of the rat

Liu, R.P.C.; Swenberg, M.-L.

doi:10.1016/0006-8993(88)90200-4

Cited by 18 publications

(13 citation statements)

References 24 publications

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“…Therefore, as proposed by Beitz (1995), ''the two schemes (divisions vs. columns) are not mutually exclusive.'' The distribution of SP R visualized in the present immunocytochemical experiments is in agreement with previous autoradiographic studies (Liu and Swenberg, 1988;Mantyh et al, 1989a). Liu and Swenberg described an uneven distribution of specific SP binding sites.…”

Section: Light Microscopic Observationssupporting

confidence: 90%

“…In the rat PAG, the presence of SP R has been demonstrated by autoradiographic localization of SP binding sites (Quirion et al, 1983;Mantyh et al, 1984;Rothman et al, 1984;Wolf et al, 1985;Mantyh and Hunt, 1986;Liu and Swenberg, 1988;Mantyh et al, 1989a;Dam et al, 1990) and in situ hybridization histochemistry (Maeno et al, 1993). More recently, the presence of SP R has been demonstrated in an immunohistochemical study in the rat PAG (Nakaya et al, 1994); however, because that study did not specifically focus on the PAG, it did not investigate the precise distribution of SP R in this region.…”

mentioning

confidence: 95%

“…In particular, in the periaqueductal gray matter (PAG) of both rats and cats, in situ hybridization and immunocytochemical techniques have shown its presence in many neurons, axons, and axon terminals (Hökfelt et al, 1977;Cuello and Kanazawa, 1978;Ljungdahl et al, 1978;Beitz, 1982;Skirboll et al, 1982;Moss and Basbaum, 1983;Gioia and Bianchi, 1988;Liu and Swenberg, 1988;Warden and Scott, 1988;Gioia and Bianchi, 1990;Li et al, 1990Li et al, , 1992Smith Guy et al, 1994).…”

mentioning

confidence: 96%

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Immunocytochemical localization of substance P receptor in rat periaqueductal gray matter: A light and electron microscopic study

Barbaresi

1998

J. Comp. Neurol.

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The distribution of substance P receptor (SPR) protein in the rat periaqueductal gray matter (PAG) was investigated with a polyclonal antibody in the four subdivisions obtained by cytochrome-oxidase histochemistry (Co-hi). At light microscopic analysis, immunoreactivity appeared particularly dense in the dorsal subdivision of the PAG, was less intense in the other subdivisions, and formed several longitudinally organized columns. SPR-like immunoreactivity (SP(R-i)) was localized mostly to cell bodies and dendrites of small and medium-sized neurons, which constituted about 6% of the total neuronal population of the PAG. At the electron microscopic level, SP(R-i) could be observed on postsynaptic as well as on nonsynaptic regions of both cell bodies and dendrites. A small proportion of axons (4.2%) and axon terminals (5.3%) showed SP(R-i), the majority of labeled axon terminals, amounting to about 70% of synapsing elements, formed asymmetric synapses with dendrites. Rare astroglial processes displaying SP(R-i) were also observed scattered throughout the neuropil of all PAG subdivisions. Our observations suggest that 1) also in the PAG, SP may act in a diffuse, nonsynaptic manner, probably on targets that are distant from its sites of release; and 2) SP may modulate excitatory neurotransmission acting presynaptically on those labeled axons that form asymmetric synapses.

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Section: Light Microscopic Observationssupporting

confidence: 90%

mentioning

confidence: 95%

mentioning

confidence: 96%

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Immunocytochemical localization of substance P receptor in rat periaqueductal gray matter: A light and electron microscopic study

Barbaresi

1998

J. Comp. Neurol.

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“…They include the opioid peptides enkephalin and dynorphin (Hökfelt et al, 1977;Uhl et al, 1979a;Finley et al, 1981a;Moss and Basbaum, 1983); substance P Liu and Swenberg, 1988); cholecystokinin (CCK) (Vanderhaeghen et al, 1980;van der Kooy et al, 1981;Vanderhaeghen, 1985); neurotensin (Uhl et al, 1979b;Jennes et al, 1982;Minagawa et al, 1983;Emson et al, 1985;Shipley et al, 1987); vasoactive intestinal polypeptide (VIP) (Sims et al, 1980;Marley et al, 1981;Hökfelt et al, 1982;Moss and Basbaum, 1983;Petit et al, 1995); somatostatin (Finley et al, 1981;Johansson et al, 1984;Priestley et al, 1993;Araneda et al, 1999); corticotropin-releasing factor (CRF) (Merchenthaler, 1984;Sakanaka et al, 1987;Commons and Valentino, 2002); neuropeptide Y (NPY) (Chronwall et al, 1985;Yamazoe et al, 1985;de Quidt and Emson, 1986); galanin (Skofitsch and Jacobowitz, 1985;Melander et al, 1986a,b); and thyrotropinreleasing hormone (TRH) (Lechan et al, 1986;Segerson et al, 1987;Tsuruo et al, 1987).…”

mentioning

confidence: 99%

Chemical neuroanatomy of the dorsal raphe nucleus and adjacent structures of the mouse brain

Maître

Fabre

et al. 2010

J of Comparative Neurology

162

141

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Serotonin neurons play a major role in many normal and pathological brain functions. In the rat these neurons have a varying number of cotransmitters, including neuropeptides. Here we studied, with histochemical techniques, the relation between serotonin, some other small-molecule transmitters, and a number of neuropeptides in the dorsal raphe nucleus (DRN) and the adjacent ventral periaqueductal gray (vPAG) of mouse, an important question being to establish possible differences from rat. Even if similarly distributed, the serotonin neurons in mouse lacked the extensive coexpression of nitric oxide synthase and galanin seen in rat. Although partly overlapping in the vPAG, no evidence was obtained for the coexistence of serotonin with dopamine, substance P, cholecystokinin, enkephalin, somatostatin, neurotensin, dynorphin, thyrotropin-releasing hormone, or corticotropin-releasing hormone. However, some serotonin neurons expressed the gamma-aminobutyric acid (GABA)-synthesizing enzyme glutamic acid decarboxylase (GAD). Work in other laboratories suggests that, as in rat, serotonin neurons in the mouse midline DRN express the vesicular glutamate transporter 3, presumably releasing glutamate. Our study also shows that many of the neuropeptides studied (substance P, galanin, neurotensin, dynorphin, and corticotropin-releasing factor) are present in nerve terminal networks of varying densities close to the serotonin neurons, and therefore may directly or indirectly influence these cells. The apparently low numbers of coexisting messengers in mouse serotonin neurons, compared to rat, indicate considerable species differences with regard to the chemical neuronatomy of the DRN. Thus, extrapolation of DRN physiology, and possibly pathology, from rat to mouse, and even human, should be made with caution.

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“…Receptors for SP have been found in the MCG of guin ea pigs [60] and rats [32,61]. The MCG contains a moder ate density of SP fibers [32,46] and receptors [60] with the highest concentration of SP receptors in the dorsal portion of the MCG [62][63][64], Photomicrographs of SP receptor immunoreactivity in the rat MCG also show an intensely labeled band of SP receptor immunoreactivity in the intercollicular ventrolateral MCG, where estrogenreceptor-containing cells have been localized in rats [3], Although species differences in SP receptors [65] and receptor distribution have been reported [60,65], some neurons in female guinea pig MCG that contain estrogen receptors may also contain receptors for SP.…”

Section: Substance P and Estrogen Receptor Immunoreactivitymentioning

confidence: 99%

Convergence of Substance P and Estrogen Receptor Immunoreactivity in the Midbrain Central Gray of Female Guinea Pigs

Turcotte

Blaustein²

1997

Neuroendocrinology

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Substance P (SP) and estrogen receptor immunoreactivity overlap in the mid-brain central gray (MCG) of female guinea pigs. Estrogen-receptor-containing cells are found throughout the rostrocaudal extent of the MCG. Moderately dense SP immunostaining is also found in this region. SP-immunoreactive punctate structures suggestive of boutons were found in close association with the processes of some estrogen-receptor-immunoreactive neurons. These associations were observed primarily in the lateral and ventrolateral MCG at the midcollicular and caudal levels. This finding suggests an anatomical substrate for interactions between SP and estradiol-sensitive neurons in the midbrain. Such interactions may underlie the effects of SP on female sexual behavior.

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Autoradiographic localization of substance P ligand binding sites and distribution of immunoreactive neurons in the periaqueductal gray of the rat

Cited by 18 publications

References 24 publications

Immunocytochemical localization of substance P receptor in rat periaqueductal gray matter: A light and electron microscopic study

Immunocytochemical localization of substance P receptor in rat periaqueductal gray matter: A light and electron microscopic study

Chemical neuroanatomy of the dorsal raphe nucleus and adjacent structures of the mouse brain

Convergence of Substance P and Estrogen Receptor Immunoreactivity in the Midbrain Central Gray of Female Guinea Pigs

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