Autoradiographic Studies of the Diurnal Rhythm of the Cell Proliferation in the Adrenal Cortex of the Rat

Ueberberg, H; W, Stolz; Trieb, G.

doi:10.1016/s0344-0338(82)80082-4

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…2B). These results are in good agreement with the findings of previous investigators that the proliferation of the cells in rat adrenal cortex has a circadian rhythm (1)(2)(3)(4)(5)(6). We then compared in detail the distribution of BrdU-labeled cells at 4 a.m. and 4 p.m., and the results obtained were statistically analyzed (Fig.…”

Section: Resultssupporting

confidence: 91%

“…The S-phase cells thus observed reached the maximum number at 4 a.m. These results confirm the previous findings of a circadian rhythm in the adrenocortical cytogenesis of rodents, with a proliferative peak at midnight (2,4), or in the morning (3,6). It has also been reported that most proliferating cells were found in an outer region of the adrenal cortex, from the subcapsular to the outer portion of zF (40)(41)(42).…”

Section: Discussionsupporting

confidence: 91%

“…ences in the animal strains used, all the investigators agreed that a circadian rhythm operated in the cell proliferation of the adrenal cortex (1)(2)(3)(4)(5)(6).…”

mentioning

confidence: 92%

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Studies on Cytogenesis in Adult Rat Adrenal Cortex: Circadian and Zonal Variations and Their Modulation by Adrenocorticotropic Hormone

Miyamoto

Mitani

Mukai

et al. 1999

Journal of Biochemistry

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Circadian rhythms and zonal variations in the cell proliferation of adult rat adrenal cortex were studied by following the cells in the DNA-synthesizing stage (S-phase) as assessed by 5-bromo-2'-deoxyuridine incorporation into the cell-nuclei and/or by visualizing proliferating cell nuclear antigen. The S-phase cells were observed throughout the day in two regions of the adrenal cortex: (i) a region from the inner half of the zona glomerulosa to near the outer margin of the zona fasciculata, and (ii) the outer one-fourth portion of the zona fasciculata. Very little change in number was observed in the former region between day and night, while a burst of cell proliferation occurred in early morning at 3-4 a.m. in the latter region. A prominent rise in the plasma adrenocorticotropic hormone (ACTH) concentration preceded the burst of cell proliferation by about 4 h. Upon raising the plasma ACTH concentration by administration of ACTH or metyrapone, prominent cell proliferation also occurred in the same portion of the zona fasciculata 4-6 h after the provoked ACTH surge. Thus at least two sites in rat adrenal cortex are responsible for cytogenesis in this endocrine organ, and respond differentially to day/night cycles and circulating ACTH levels.

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Section: Resultssupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

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Studies on Cytogenesis in Adult Rat Adrenal Cortex: Circadian and Zonal Variations and Their Modulation by Adrenocorticotropic Hormone

Miyamoto

Mitani

Mukai

et al. 1999

Journal of Biochemistry

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“…Further investigation on 4-to 5-week old mice (Halberg et al 1957) documented an overall peak around midnight. However, Nouët & Michat (1977) proposed maximal activity between midnight and 06.00 h. Ueberberg et al (1982) identified a peak in the zona fasciculata (ZF) between 09.00 and 12.00 h with no rhythm in the zona glomerulosa (ZG). In con¬ trast, Dobrokhotov & Nikanorova (1962) found a peak in ZF between 07.00 and 13.00 h, but also demonstrated a rhythm in ZG, with maximal activity at 19.00 to 22.00 h.…”

Section: Discussionmentioning

confidence: 99%

“…Circadian rhythms of proliferative activity have been reported in various endocrine organs including the thyroid gland (Wynford- Thomas, Stringer & Williams, 1982), pituitary gland (Nouët & Kujas, 1975) and adrenal cortex (Halberg, Frantz & Bittner, 1957;Kirillov & Kurilenko, 1977;Ueberberg, Stolz & Trieb, 1982). It is often assumed that such rhythms of growth are related to the functional rhythms of the gland, but circadian variations in proliferation also occur in tissues without apparent functional rhyth¬ micity, including skin and cornea (Scheving & Pauly, 1960.…”

Section: Introductionmentioning

confidence: 99%

Age-related variation in circadian rhythms of mitosis in the adrenal cortex of the male rat

McNicol

Penman²,

Duffy³

1989

Journal of Endocrinology

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Using a metaphase arrest technique, mitotic activity was quantified in the adrenal cortex over a 24-h period in 14-day-old male Sprague-Dawley rats before functional rhythmicity of the hypothalamic pituitary-adrenal (HPA) axis is established, and after its onset, in 6- to 7-week-old rats. At all times, proliferative activity was greater in the younger animals, as previously reported. A significant circadian rhythm was identified in both groups, but the timing of the peak differed, lying between 17.00 and 21.00 h at 14 days and 11.00 and 15.00 h at 6-7 weeks. These results raise the possibility that functional rhythmicity of the HPA axis may alter an inherent proliferative rhythm.

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Density separation of rat adrenocortical cells: Morphology, steroidogenesis, and P-450scc expression in primary culture

Roskelley

Auersperg

1990

In Vitro Cell Dev Biol

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We have developed a method that separates rat adrenocortical cells by density into populations which retain zone specific properties in primary culture. Two different parenchymal populations were obtained and designated 2FASC (1.034 g/ml, 18.0 microns cell diameter) and 7GLOM (1.069 g/ml, 11.7 microns cell diameter). In freshly isolated cell suspensions the physical characteristics and differential steroidogenic responses to adrenocorticotropin and angiotensin II suggested that 2FASC cells originated predominantly from the zona fasciculata and 7GLOM cells from the zona glomerulosa. In primary culture (Dulbecco's Modified Eagle's Medium-F12 medium with 15% horse serum and 2.5% fetal bovine serum) the two populations exhibited different morphologies. 2FASC cells retained lipid and formed cohesive epithelial monolayers that remained stationary for 3 wk. 7GLOM cells were initially epithelial but rapidly lost lipid, spread, and assumed fibroblastic shapes. Both cell types were positive for the cholesterol side-chain cleavage cytochrome P-450 by immunofluorescence. Therefore, the morphologic changes seen in 7GLOM cultures were due to modulation, not fibroblastic overgrowth. This phenotypic plasticity may reflect the mesodermal origin of the adrenal cortex, and the subcapsular location of 7GLOM cells in vivo. In contrast, cells such as 2FASC which are located deeper in the cortex seem to have a more restricted, fully committed parenchymal phenotype.

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Autoradiographic Studies of the Diurnal Rhythm of the Cell Proliferation in the Adrenal Cortex of the Rat

Cited by 10 publications

References 11 publications

Studies on Cytogenesis in Adult Rat Adrenal Cortex: Circadian and Zonal Variations and Their Modulation by Adrenocorticotropic Hormone

Studies on Cytogenesis in Adult Rat Adrenal Cortex: Circadian and Zonal Variations and Their Modulation by Adrenocorticotropic Hormone

Age-related variation in circadian rhythms of mitosis in the adrenal cortex of the male rat

Density separation of rat adrenocortical cells: Morphology, steroidogenesis, and P-450scc expression in primary culture

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