Autoradiographic study of RNA and protein synthesis in sectioned peripheral nerves

Benech, Claudio R.; Sotelo, Julio; Menéndez, José A.; Correa-Luna, R.

doi:10.1016/0014-4886(82)90102-9

Cited by 26 publications

(26 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data supporting intercellular transport of glial RNA to the neuron soma have also been corroborated by cytospectrophotometric methods that may hardly be blamed for confusing a glial cell with a dendrite (260,261). As reviewed in this article, intercellular transport of newly synthesized RNA from periaxonal glia to the axon has been demonstrated in vertebrate (25,80,177,280) and invertebrate species (9,62,92,117,229,265), as well as between perisynaptic cells and squid nerve terminals (92,116).…”

Section: Commentarymentioning

confidence: 60%

“…In vivo incorporation of locally applied radiolabeled amino acids in the axons of lesioned and unlesioned rat sciatic nerves was demonstrated by autoradiographic methods (25,53). More recently, with the use of RT-PCR methods, intact and axotomized sciatic nerves of adult rats were shown to contain the mRNAs encoding all NF proteins.…”

Section: Vertebrate Axonsmentioning

confidence: 99%

“…In the peripheral nervous system of rats, the local synthesis of axonal RNA (and protein) was demonstrated by quantitative autoradiography of proximal stumps of axotomized sciatic nerves in vivo (25). Following 30-min incubation with radiolabeled precursors, newly synthesized RNA was present in Schwann cells, myelin sheaths, and axons in order of decreasing concentrations.…”

Section: B Vertebrate Axonsmentioning

confidence: 99%

See 2 more Smart Citations

Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit

Giuditta¹,

Chun²,

Eyman³

et al. 2008

Physiological Reviews

View full text Add to dashboard Cite

Neurons have complex and often extensively elongated processes. This unique cell morphology raises the problem of how remote neuronal territories are replenished with proteins. For a long time, axonal and presynaptic proteins were thought to be exclusively synthesized in the cell body, which delivered them to peripheral sites by axoplasmic transport. Despite this early belief, protein has been shown to be synthesized in axons and nerve terminals, substantially alleviating the trophic burden of the perikaryon. This observation raised the question of the cellular origin of the peripheral RNAs involved in protein synthesis. The synthesis of these RNAs was initially attributed to the neuron soma almost by default. However, experimental data and theoretical considerations support the alternative view that axonal and presynaptic RNAs are also transcribed in the flanking glial cells and transferred to the axon domain of mature neurons. Altogether, these data suggest that axons and nerve terminals are served by a distinct gene expression system largely independent of the neuron cell body. Such a local system would allow the neuron periphery to respond promptly to environmental stimuli. This view has the theoretical merit of extending to axons and nerve terminals the marginalized concept of a glial supply of RNA (and protein) to the neuron cell body. Most long-term plastic changes requiring de novo gene expression occur in these domains, notably in presynaptic endings, despite their intrinsic lack of transcriptional capacity. This review enlightens novel perspectives on the biology and pathobiology of the neuron by critically reviewing these issues.

show abstract

Section: Commentarymentioning

confidence: 60%

Section: Vertebrate Axonsmentioning

confidence: 99%

Section: B Vertebrate Axonsmentioning

confidence: 99%

See 1 more Smart Citation

Local Gene Expression in Axons and Nerve Endings: The Glia-Neuron Unit

Giuditta¹,

Chun²,

Eyman³

et al. 2008

Physiological Reviews

View full text Add to dashboard Cite

show abstract

“…The existence of a local response by the injured nerve characterized by an increase in the amino acid incorporation into TCA precipitable fraction has been reported [Benech et al, 1982]. This phenomenon that is maximal about 24 h after injury and occurring mainly in the proximal stump region (Fig.…”

Section: Discussionmentioning

confidence: 94%

“…It is also demonstrated that both the messengers coding for myosin V and the radiolabeled protein are increased in injured nerves compared to intact ones. This result might means that myosin V is one of the several proteins that are synthesized after local injury [Benech et al, 1982;Sotelo et al, 1992;Sotelo-Silveira et al, 2000]. In any case, at the same time, there are indications that Myosin Va might also take part in the resulting plastic changes within the nerve.…”

Section: ϩmentioning

confidence: 88%

Myosin Va is locally synthesized following nerve injury

Calliari

Sotelo‐Silveira

Costa

et al. 2002

Cell Motil. Cytoskeleton

Self Cite

View full text Add to dashboard Cite

The presence of Myosin Va (an actin-based molecular motor) in the peripheral nervous system was examined and its subcellular distribution within the axons of the sciatic nerve was demonstrated via immunocytochemistry. Myosin Va (M-Va) in the nerve was detected by using SDS-PAGE and Western blot techniques with a polyclonal antibody specifically raised against the M-Va globular tail domain. In addition, purification of M-Va from the rat sciatic nerve prior to immunoblotting yielded a M-Va standard band. Likewise, optical immunocytochemical procedures revealed the presence of M-Va, particularly in the cortical axoplasmic territory, but also in the Schwann cell soma. The above experiments were carried out both on intact as well as on severed sciatic nerves with similar results. The proximal stumps of severed sciatic nerves (from 0 to 72 h after injury) were labelled in vivo with (35)S-methionine. SDS-PAGE autoradiography of the immunoabsorbed M-Va from the radiolabelled homogenized nerve tissue showed a significant increment of the radioactive intensity of M-Va heavy chain band through time. Moreover, a significant increment of transcripts coding for M-Va heavy chain was detected through time using RT-PCR after nerve injury and compared to intact nerves. This data suggest that M-Va is up-regulated in a time-dependent manner. The latter suggests a possible involvement of M-Va in nerve regeneration processes.

show abstract