Autoradiography with the Electron Microscope: Experimental Techniques and Considerations Using Plant Tissues

Pickett‐Heaps, Jeremy D.

doi:10.1007/978-3-642-87496-3_7

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…The film was gelled and dry so that the silver bromide grains did not redistribute according to the contours of the specimen, and possible movement of label within the specimen was prevented. To achieve this, a layer of emulsion was formed on the smooth flawless surface of a block of agar (Pickett-Heaps, 1972) previously coated with a thin layer of Formvar to act as a release layer.…”

Section: On Artificial Surfacesmentioning

confidence: 99%

An autoradiographic method for determining nutrient competition between leaf epiphytes and plant pathogens

Edwards

Blakeman

1984

Journal of Microscopy

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SUMMARY A method is described for the study of nutrient competition between leaf surface microorganisms and plant pathogenic fungi. Autoradiography was used to investigate the uptake of water‐soluble 14C‐labelled nutrients by applying a dry pre‐formed layer of emulsion to the specimen. The method was adapted for use on both leaf and artificial surfaces. A photometric method was used for rapid examination of autoradiographs.

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Section: On Artificial Surfacesmentioning

confidence: 99%

An autoradiographic method for determining nutrient competition between leaf epiphytes and plant pathogens

Edwards

Blakeman

1984

Journal of Microscopy

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“…Pickett-Heaps (1972) published two micrographs of gelled emulsions applied to sections (loop method) in which he demonstrated non-random distribution. However, the liability of gelled emulsion can be corrected by use of a support film of formvar (Pickett-Heaps, 1972).…”

Section: Coated Sectionsmentioning

confidence: 99%

“…The emulsion film has been satisfactorily produced by the loop method (Caro & van Tubergen, 1962;Pickett-Heaps, 1972;Hendrickson, 1975). However, variables such as bath temperature, emulsion dilution, size of the wire Coatirg sections for EM autoradiography loop, thickness of the wire, air temperature, humidity of the room, and movement of air make reproducibility of this method difficult, The stripping technique for liquid emulsion was developed to simplify the manipulations in the coating procedure and to insure reproducibility.…”

Section: Forniation and Application Of Thz Emulsion Jilntmentioning

confidence: 99%

Coating sections for electron microscopic autoradiography: a stripping technique using liquid emulsion

Burry

Lasher

1975

Journal of Microscopy

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SUMMARY A coating technique for electron microscope autoradiography is described which combines the advantage of forming an emulsion film by a dipping method with the ease of coating sections already on grids. Sections are coated so that a formvar support film separates the section and the emulsion crystals. This intermediate layer of formvar ensures a random distribution of the emulsion crystals. Using light gold sections, Ilford L‐4 emulsion and Microdol‐X development, the resolution of this technique, as determined by the half distance method, was 150 nm. The additional layer of formvar slightly reduced the image quality with biological samples in the electron microscope. This technique has a minimal loss of resolution and image quality for moderate resolution electron microscope autoradiography.

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“…Autoradiographic electron microscopy differs from that used in light microscopy primarily in sophistication of emulsion composition and application (74). Silver grains developed after exposure to radiation can be viewed as electron dense spots over thin sections in the TEM or detected via X-ray analysis in TEM, SEM or MP (12).…”

Section: Instrument Type and Information Obtainablementioning

confidence: 99%

Electron Optics: Principles, Techniques, and Applications in Horticulture1

Rasmussen

Hooper

1974

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Excellent, though limited, use has been made of electron optical equipment in the field of horticulture. The increasing availability and the refinements in such instruments as the electron microprobe X-ray analyzer, the scanning electron microscope and the transmission electron microscope have made them invaluable tools for the horticultural scientist. In an effort to extend the use of such equipment this paper discusses the operation, present and potential applications, sample preparation techniques and problems, and a glimpse of the future of electron optical equipment in horticulture.

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Autoradiography with the Electron Microscope: Experimental Techniques and Considerations Using Plant Tissues

Cited by 6 publications

References 40 publications

An autoradiographic method for determining nutrient competition between leaf epiphytes and plant pathogens

An autoradiographic method for determining nutrient competition between leaf epiphytes and plant pathogens

Coating sections for electron microscopic autoradiography: a stripping technique using liquid emulsion

Electron Optics: Principles, Techniques, and Applications in Horticulture1

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