Autoschizis: A Mode of Cell Death of Cancer Cells Induced by a Prooxidant Treatment<i>In Vitro</i>and<i>In Vivo</i>

Gilloteaux, Jacques; Jamison, James M.; Arnold, David; Summers, Jack L.

doi:10.1002/9781119432463.ch28

Cited by 5 publications

(2 citation statements)

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“…Thus, organelle-free cytoplasm progressively diminishes through a series of self-excisions due to lipid peroxidation. Also the nucleus becomes smaller; most organelles surround a small intact nucleus in a narrow rim of cytoplasm [ 60 – 62 ]. Interestingly, there are several lines of evidences which indicate the killing of mycobacteria via single members of reactive oxygen species (nitric oxide [ 63 ], peroxide [ 64 ] and reactive nitrogen intermediates [ 65 ]).…”

Section: Discussionmentioning

confidence: 99%

Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells

et al. 2016

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BackgroundMycobacterial infections remain a significant cause of morbidity and mortality worldwide. Due to limitations of the currently available model systems, there are still comparably large gaps in the knowledge about the pathogenesis of these chronic inflammatory diseases in particular with regard to the human host. Therefore, we aimed to characterize the initial phase of mycobacterial infections utilizing a human ex vivo lung tissue culture model designated STST (Short-Term Stimulation of Tissues).MethodsHuman lung tissues from 65 donors with a size of 0.5–1 cm3 were infected each with two strains of three different mycobacterial species (M. tuberculosis, M. avium, and M. abscessus), respectively. In order to preserve both morphology and nucleic acids, the HOPE® fixation technique was used. The infected tissues were analyzed using histo- and molecular-pathological methods. Immunohistochemistry was applied to identify the infected cell types.ResultsMorphologic comparisons between ex vivo incubated and non-incubated lung specimens revealed no noticeable differences. Viability of ex vivo stimulated tissues demonstrated by TUNEL-assay was acceptable. Serial sections verified sufficient diffusion of the infectious agents deep into the tissues. Infection was confirmed by Ziel Neelsen-staining and PCR to detect mycobacterial DNA. We observed the infection of different cell types, including macrophages, neutrophils, monocytes, and pneumocytes-II, which were critically dependent on the mycobacterial species used. Furthermore, different forms of nuclear alterations (karyopyknosis, karyorrhexis, karyolysis) resulting in cell death were detected in the infected cells, again with characteristic species-dependent differences.ConclusionWe show the application of a human ex vivo tissue culture model for mycobacterial infections. The immediate primary infection of a set of different cell types and the characteristic morphologic changes observed in these infected human tissues significantly adds to the current understanding of the initial phase of human pulmonary tuberculosis. Further studies are ongoing to elucidate the molecular mechanisms involved in the early onset of mycobacterial infections in the human lung.

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Section: Discussionmentioning

confidence: 99%

Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells

et al. 2016

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“…discovered as an induced cancer necrosis triggered by Vitamin C and K3 treatments [27,28], which is morphologically characterized by plasma membrane damage, organelle-free cytoplasm, progressive self-excisions of cytoplasm (autoschizic bodies) to perikaryon, intact nuclear envelope and karyolysis [29,30]. Comparing to P1, the DNA amounts, mitochondria amounts and membrane potentials were verified to be low in P2 (Figure 2B, C).…”

Section: Glioma-derived Fsc Low Necrotic Particles Are Autoschizis-like Products (Alps)mentioning

confidence: 99%

Glioma stem cell (GSC)-derived autoschizis-like products confer GSC niche properties involving M1-like tumor-associated macrophages

et al. 2020

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Hevea brasiliensis latex dialysed C-serum precipitate subfraction exerts a negligible level of genotoxicity in the Ames test, mouse lymphoma assay and micronucleus assay

et al. 2023

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Autoschizis: A Mode of Cell Death of Cancer Cells Induced by a Prooxidant TreatmentIn VitroandIn Vivo

Cited by 5 publications

References 300 publications

Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells

Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells

Glioma stem cell (GSC)-derived autoschizis-like products confer GSC niche properties involving M1-like tumor-associated macrophages

Hevea brasiliensis latex dialysed C-serum precipitate subfraction exerts a negligible level of genotoxicity in the Ames test, mouse lymphoma assay and micronucleus assay

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