Autotetraploid Coffea canephora and Auto-Alloctaploid Coffea arabica From In Vitro Chromosome Set Doubling: New Germplasms for Coffea

Venial, Lucimara Ribeiro; Mendonça, Maria Andréia Corrěa; Amaral-Silva, Paulo Marcos; Canal, Guilherme Bravim; Passos, Ana Beatriz Rocha de Jesus; Ferreira, Adésio; Soares, Taís Cristina Bastos; Clarindo, Wellington Ronildo

doi:10.3389/fpls.2020.00154

Cited by 12 publications

(18 citation statements)

References 51 publications

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“…In comparison with the doubling agents, oryzalin was found to show higher frequency of the induced polyploidy over colchicine [23], but it inhibited the callus growth and seedling regeneration. Liquid medium was more effective in inducing the polyploidy when compared with semi solid media [7,23]. A concentration of 5 µm oryzalin was found highly effective over colchicine and trifluralin in inducing chromosome set doubling of calli obtained from interspecific hybrid plants of elephant grass × pearl millet [24].…”

Section: Production Of Polyploid Plantsmentioning

confidence: 94%

“…Polyploid plants (polysomic polyploids, true and segmental amphidiploids) can be produced using various chemicals, such as colchicine, amiprophos-methyl, trifluralin or oryzalin; or physical methods such as temperature shock and protoplast fusion [8]. The most common chemical is colchicine obtained from Colchicum autumnale, which has an inhibitory effect on the spindle formation, causing a failure during anaphase disjunction [8] and during cytokinesis [7], resulting in one cell with the doubled chromosome set. Hence, colchicine treatment results in pure polyploid individuals and impure (mixoploid or with aneuploid cells), depending upon the colchicine concentration and exposure time, plant tissues and its development stage, and method for chromosome set doubling.…”

Section: Production Of Polyploid Plantsmentioning

confidence: 99%

“…The polyploids are also used as bridging species to transfer resistant genes from their parental species, as genes related to biotic and abiotic stresses [4,5]. In contrast to natural polyploidy that have evolved as products of natural selection [6], induced polyploid (polysomic polyploids, and true or segmental O-(2-nitro-p-tolyl) N-isopropyl phosphora midothionate) [7]. Besides, some polyploids have been also obtained after using physical agents, such as temperature shock, and by intra-(polysomic polyploidy) or interspecific (true or segmental amphiploidy) crossing.…”

Section: Introductionmentioning

confidence: 99%

“…Artificial polyploidy was developed in 1937 after discovery and extraction of the natural compound named as colchicine. The colchicine has the ability to inhibit spindle fiber formation to arrest the chromosome, causing a failure of chromatid disjunction [8] and of cytokinesis [7].…”

Section: Introductionmentioning

confidence: 99%

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Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Rauf¹,

Ortíz²,

Malinowski³

et al. 2021

Preprint

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Polyploidy is a condition of having more than two sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological methods (2n gametes). It is considered an interesting approach due to increased genescope and expression, thus resulting in phenotypic changes, such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages from optimized application methods, concentration and time mainly using colchicine. These experimental polyploids proved to be a valuable tool for understanding gene expression that is driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers aided on the identification of rare alleles for particular loci when compared with diploids, which may explain their heterozygosity, phenotypic plasticity and adaptability to diverse environments. It has also been observed that experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g. leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids underwent selection for several generations to improve their performance and stability.

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Section: Production Of Polyploid Plantsmentioning

confidence: 94%

Section: Production Of Polyploid Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Rauf¹,

Ortíz²,

Malinowski³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Polyploids are also used as bridge species to transfer resistant genes from their parental species [4,5]. In contrast to natural polyploidy arising from natural selection [6], induced polyploid (polysomic polyploids, and true or segmental amphidiploids mainly result from the anti-tubulin effect of colchicine ((S)-N-(5,6,7,9-tetrahydro-1,2,3,10-tetramethoxy-9-oxobenzo[a]heptalen-7-yl) acetamide; and also of oryzalin (3,5-dinitro-N 4 ,N 4 -dipropylsulfanilamide), trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N,-dipropyl-p-toluidine) and amiprophos methyl (O-methyl O-(2-nitro-p-tolyl) N-isopropyl phosphoramidothionate) [7].…”

Section: Introductionmentioning

confidence: 99%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

Self Cite

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Polyploidy means having more than two basic sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological (2n gametes) methods. This approach allows an increased gene scope and expression, thus resulting in phenotypic changes such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects that are partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages after optimizing methods, concentration and time, particularly when using colchicine. These experimental polyploids are a valuable tool for understanding gene expression, which seems to be driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers facilitated the identification of rare alleles for particular loci when compared with diploids, and also explained their heterozygosity, phenotypic plasticity and adaptability to diverse environments. Experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g., leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids should undergo selection for several generations to improve their performance and stability.

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