The application of improperly treated compost is one of the causes of bacterial contamination in the field, crops, food and water. Although there are practical guidelines for treatment of compost published, there could be a misapplication in local situations, such as lack of attention by traditional users, labour ineffectiveness and material unavailability. Considering these setbacks, farmers may unknowingly use improperly treated compost as fertilizer. The objectives of this study were (i) to characterize the die-off represented by kinetic inactivation rate coefficient, k (h -1 ) of Enterococcus in clay and sandy loam soils, (ii) to determine the effect of temperature, compost-to-soil ratio and soil type on the inactivation rates of Enterococcus. The soils were amended with compost at a ratio of 1 part compost to 10 parts of soil 1:10, 1:25, 1:50 and 1:100 held at different temperatures (30°C, 40°C and 50°C). The moisture content was set to 25% in all conditions. Enterococcus is enteric bacteria and not always pathogenic but an indicator of pathogenic bacteria. Inactivation of Enterococcus (pathogenic bacteria) in the soil with high temperature under different compost application rates was tried in the laboratory test. As a result, (1) the inactivation rates of Enterococcus in clay soils were 0.015-0.027 h -1 , 0.246-0.322 h -1 , 0.397-0.571 h -1 for 30, 40 and 50°C respectively. Sandy loam soils were 0.056-0.130 h -1 , 0.348-0.447 h -1 and 0.475-0.630 h -1 for 30, 40 and 50°C respectively, (2) inactivation rates of Enterococcus in soils amended with compost from the composting toilet depended on temperature and soil type but not on the compost-to-soil ratios. The experimental conditions in this study are different from the real field situation because temperature and humidity changes over time under field conditions. Therefore, care must be taken when interpreting the results of this study to estimate the die-off rates in real field conditions. Further research is required to simulate the behaviour of pathogenic bacteria in field conditions of a hot semi-arid climate.