Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars

Pan, Zhizhong; Guo, Yongze; Ding, Shilei; Song, Zhen; Cui, Hanyuan; Zhang, Yue; Zhang, Zhijun; Chen, Xiaoming

doi:10.3390/molecules27196734

Cited by 7 publications

(1 citation statement)

References 30 publications

(34 reference statements)

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“…Traditional Chinese medicine has long recognized the value of HE, leading to its widespread application in various therapeutic contexts. Recent pharmacological investigations have unveiled the presence of polysaccharides, triterpenes, fatty acids, and other bioactive compounds in HE, highlighting its potential for anti-ulcer, anti-inflammatory, and immune modulation effects, as well as blood sugar and lipid regulation [3][4][5][6][7][8]. Consequently, the extraction of these valuable components from HE has become a focal point for research and development [9].…”

Section: Introductionmentioning

confidence: 99%

Enrichment Extraction and Activity Study of the Different Varieties of Hericium erinaceus against HCT-8 Colon Cancer Cells

Cao,

Zhang,

Wei

et al. 2023

Molecules

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Hericium erinaceus (HE), a widely utilized natural remedy and dietary source, has garnered significant attention for its therapeutic potential in various diseases. In this study, we employed supercritical fluid extraction (SFE) technology to isolate the bioactive compounds from HE’s fruiting body. Comprehensive assessments of the antioxidant and antibacterial activities were conducted, along with in vitro investigations on the human colon cancer cell line (HCT-8). The SFE rate served as the evaluation metric, while the variables of extraction time, pressure, and temperature were systematically examined. By integrating the response surface center composite design, we successfully optimized the extraction process, yielding optimal parameters of 80 min, 30 MPa, and 35 °C, thus resulting in an extraction rate of 2.51%. These optimized conditions exhibited considerable antioxidant capacity, anticancer activity, and antibacterial potential. Furthermore, we employed graded alcohol extraction to refine the crude extracts, thereby confirming superior anticancer effects under a 70% alcohol precipitation. To elucidate the composition, Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) were employed to analyze the crude extracts and isolates of HE, facilitating a comparative analysis of six HE varieties. Our findings suggest that sterol derivatives hold promise as the active component against the colon cancer HCT-8 cell line. In conclusion, this study underscores the potential of HE SFE in the development of functional foods or alternative drugs for colon cancer treatment, thus opening new avenues for therapeutic interventions.

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Section: Introductionmentioning

confidence: 99%

Enrichment Extraction and Activity Study of the Different Varieties of Hericium erinaceus against HCT-8 Colon Cancer Cells

Cao,

Zhang,

Wei

et al. 2023

Molecules

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The impact of continuous cultivation of Ganoderma lucidum on soil nutrients, enzyme activity, and fruiting body metabolites

Ji,

Zhang,

et al. 2024

Sci Rep

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To explore the impacts of continuous Ganoderma lucidum cultivation on soil physicochemical factors, soil enzyme activity, and the metabolome of Ganoderma lucidum fruiting bodies, this study conducted two consecutive years of cultivation on the same plot of land. Soil physicochemical factors and enzyme activity were assessed, alongside non-targeted metabolomic analysis of the Ganoderma lucidum fruiting bodies under continuous cultivation. The findings unveiled that in the surface soil layer (0–15 cm), there was a declining trend in organic matter, ammonium nitrogen, available phosphorus, available potassium, pH, polyphenol oxidase, peroxidase, alkaline phosphatase, and sucrase, whereas nitrate nitrogen, electrical conductivity (EC), and salt content exhibited an upward trend. Conversely, in the deeper soil layer (15–30 cm), organic matter, ammonium nitrogen, available potassium, alkaline phosphatase, and sucrase demonstrated a decreasing trend, while nitrate nitrogen, available phosphorus, pH, EC, salt content, polyphenol oxidase, and soil peroxidase showed an increasing trend. Metabolomic analysis of Ganoderma lucidum fruiting bodies distinguished 64 significantly different metabolites between the GCK and GT groups, with 39 components having markedly higher relative contents in GCK and 25 components having significantly lower relative contents in GCK compared to GT. Moreover, among these metabolites, there were more types with higher contents in the fruiting bodies harvested in the first year (GCK) compared to those harvested in the second year (GT), with pronounced differences. KEGG pathway analysis revealed that GCK exhibited more complex metabolic pathways compared to GT. The metabolites of Ganoderma lucidum fruiting bodies were predominantly influenced by soil physicochemical factors and soil enzyme activity. In the surface soil layer (0–15 cm), the metabolome was significantly affected by soil pH, soil organic matter, available phosphorus, and soil alkaline phosphatase, while in the deeper soil layer (15–30 cm), differences in the Ganoderma lucidum metabolome were more influenced by soil alkaline phosphatase, soil catalase, pH, nitrate nitrogen, and soil sucrase.

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Study on the use of Imazalil to continuous cropping obstacle of Ganoderma lucidum caused by Xylogone ganodermophthora

Huang,

Lu,

Cao

et al. 2024

Chem. Biol. Technol. Agric.

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Background The Xylogone ganodermophthora is a pathogenic bacterium that poses a significant challenge to the continuous cultivation of the Ganoderma lucidum fungus. This study aims to identify and investigate specific agents for the effective prevention and control of X. ganodermophthora, establishing a theoretical foundation for overcoming this persistent challenge in G. lucidum cultivation. Results Using different G. lucidum soil as materials to study the presence of X. ganodermophthora in the soil. Additionally, the plate confrontation test was employed to investigate the impact of X. ganodermophthora on G. lucidum growth. The impact of physical factors and antibacterial agents on pathogenic bacteria was successfully carried out, with a further exploration of the effectiveness of field control. PCR amplification experiment and sequencing analysis verified that X. ganodermophthora existed in G. lucidum continuous cropping obstacle soil. This pathogenic bacteria has a significant inhibitory effect on the growth of G. lucidum, with an inhibition rate of up to 52.23%. High temperature, low temperature, light and other physical factors have no obvious inhibitory effect on this pathogen. Further investigation revealed that specific drugs, such as low concentrations (10 μL/mL) of Acticide DB20 and Imazalil, could effectively inhibit X. ganodermophthora growth in G. lucidum. Among them, Imazalil has a notable inhibitory effect on the growth of X. ganodermophthora. Conclusions Indoor toxicity test and field control results showed that Imazalil could effectively control the growth of pathogen X. ganodermophthora in G. lucidum continuous cropping obstacle, and promote the growth of G. lucidum. Graphical Abstract

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Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars

Cited by 7 publications

References 30 publications

Enrichment Extraction and Activity Study of the Different Varieties of Hericium erinaceus against HCT-8 Colon Cancer Cells

Enrichment Extraction and Activity Study of the Different Varieties of Hericium erinaceus against HCT-8 Colon Cancer Cells

The impact of continuous cultivation of Ganoderma lucidum on soil nutrients, enzyme activity, and fruiting body metabolites

Study on the use of Imazalil to continuous cropping obstacle of Ganoderma lucidum caused by Xylogone ganodermophthora

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