Autotrophic growth and lipid production of Chlorella sorokiniana in lab batch and BIOCOIL photobioreactors: Experiments and modeling

Concas, Alessandro; Malavasi, Veronica; Costelli, Cristina; Fadda, Paolo; Pisu, Maria Giuseppina; Cao, Giacomo

doi:10.1016/j.biortech.2016.03.089

Cited by 30 publications

(11 citation statements)

References 33 publications

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“…Selection of an optimal green alga strain as a negative‐control for extracellular sugar production is hindered in part based on the small numbers of algal genomes that are available and a very limited understanding of the strains that produce extracellular sugars. Thus, C. sorokiniana UTEX 1602 was selected from within our own laboratory stocks as we had determined that it does not produce appreciable amounts of extracellular sugars under similar conditions to what is found for M. conductrix SAG 241.80, and C. sorokiniana species are commonly used as a model species (Hu et al ., ; Barney et al ., ; Concas et al ., ; Li et al ., ; Wang et al ., ). In addition, C. sorokiniana UTEX 1602 is a model for symbiotic associations with certain bacteria (de‐Bashan et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

et al. 2018

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Green algae represent a key segment of the global species capable of photoautotrophic-driven biological carbon fixation. Algae partition fixed-carbon into chemical compounds required for biomass, while diverting excess carbon into internal storage compounds such as starch and lipids or, in certain cases, into targeted extracellular compounds. Two green algae were selected to probe for critical components associated with sugar production and release in a model alga. Chlorella sorokiniana UTEX 1602 - which does not release significant quantities of sugars to the extracellular space - was selected as a control to compare with the maltose-releasing Micractinium conductrix SAG 241.80 - which was originally isolated from an endosymbiotic association with the ciliate Paramecium bursaria. Both strains were subjected to three sequencing approaches to assemble their genomes and annotate their genes. This analysis was further complemented with transcriptional studies during maltose release by M. conductrix SAG 241.80 versus conditions where sugar release is minimal. The annotation revealed that both strains contain homologs for the key components of a putative pathway leading to cytosolic maltose accumulation, while transcriptional studies found few changes in mRNA levels for the genes associated with these established intracellular sugar pathways. A further analysis of potential sugar transporters found multiple homologs for SWEETs and tonoplast sugar transporters. The analysis of transcriptional differences revealed a lesser and more measured global response for M. conductrix SAG 241.80 versus C. sorokiniana UTEX 1602 during conditions resulting in sugar release, providing a catalog of genes that might play a role in extracellular sugar transport.

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Section: Introductionmentioning

confidence: 99%

Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

et al. 2018

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“…In addition to nutrients, the uptake of organic and/or inorganic carbon should also be considered carefully (included in 26% of articles). Carbon uptake is commonly modeled using Michaelis-Menten or Haldane kinetics, depending on whether or not the compound can be inhibitory at higher concentrations (Baroukh et al., 2017) and on the amount of existing internal carbon stores (Concas et al., 2016; Guest et al., 2013). Apart from uptake, carbon utilization is dependent on several interrelated factors that must be considered concurrently.…”

Section: Mechanistic Modeling Of Phytoplanktonmentioning

confidence: 99%

Microalgae and cyanobacteria modeling in water resource recovery facilities: A critical review

Shoener

Schramm

Béline³

et al. 2019

Water Research X

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Microalgal and cyanobacterial resource recovery systems could significantly advance nutrient recovery from wastewater by achieving effluent nitrogen (N) and phosphorus (P) levels below the current limit of technology. The successful implementation of phytoplankton, however, requires the formulation of process models that balance fidelity and simplicity to accurately simulate dynamic performance in response to environmental conditions. This work synthesizes the range of model structures that have been leveraged for algae and cyanobacteria modeling and core model features that are required to enable reliable process modeling in the context of water resource recovery facilities. Results from an extensive literature review of over 300 published phytoplankton models are presented, with particular attention to similarities with and differences from existing strategies to model chemotrophic wastewater treatment processes (e.g., via the Activated Sludge Models, ASMs). Building on published process models, the core requirements of a model structure for algal and cyanobacterial processes are presented, including detailed recommendations for the prediction of growth (under phototrophic, heterotrophic, and mixotrophic conditions), nutrient uptake, carbon uptake and storage, and respiration.

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“…from the storage compounds (both starch and lipid) in the dark period which were accumulated during the light period. On the other hand, although some microalgae strains undergo mitosis preferably during the night in order to prevent DNA photo‐damage (Concas et al, ). There is some evidence that shows some strains continue their cell division under prolonged light exposure (Tang et al, ; Zachleder, Kateřina, Vítová, & Hendrychová, ) which led to higher numbers of sequences of reproductive processes, biomass content, biomass productivity, and FAME (Fatty acid methyl ester)/DW.…”

Section: Discussionmentioning

confidence: 99%

“…The decrease of OD 680 suggests the chlorophyll degraded (Msanne et al, ) potentially for nutrient recycling under nitrogen starvation. Since microalgae utilize a certain fraction of the assimilated nitrogen for new molecules of chlorophyll‐a biosynthesis, the chlorophyll‐a synthesis rate is strictly related to the nitrogen uptake rate and can be expressed by the mass balance for the chlorophyll cell quota (Concas et al, ). Formation of storage compounds cause an increase of dry weight but did not contribute to extra light absorption at 680 nm.…”

Section: Discussionmentioning

confidence: 99%

Effective role of medium supplementation in microalgal lipid accumulation

Danesh

Mooij

Ebrahimi

et al. 2018

Biotech & Bioengineering

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The present study investigated the interaction between starch and lipid accumulation in a green microalgae enrichment culture. The objective was to optimize the lipid content by manipulation of the medium in regular batch culture. Two medium designs were evaluated: First a high ortho-P concentration with vitamin supplementary (Pi-vitamins supplemented medium), second normal growth medium (control). Both media contained a low amount of nitrogen which was consumed during batch growth in three days. The batch experiments continued for another 4 days with the absence of soluble nitrogen in the medium. When the mixed microalgal culture was incubated in the Pi-vitamin supplemented medium, the lipid, and starch content of the culture increased within the first 3 days to 102.0 ± 5.2 mg/L (12.7 ± 0.6% of DW) and 31.7 ± 1.6 mg/L (4.0 ± 0.2% of DW), respectively. On the last day of the experiment, the lipid, and starch content in Pi-vitamin medium increased to 663.1 ± 32.5 mg/L (33.4 ± 1.6% of DW) and 127.5 ± 5.2 mg/L (6.4 ± 0.3% of DW). However, the lipid and starch content in the control process, reached to 334.7 ± 16.4 mg/L (20.1 ± 1.0% of DW) and 94.3 ± 4.6 mg/L (5.7 ± 0.3% of DW), respectively. The high Pi-vitamin medium induced storing lipid formation clearly while the starch formation was not affected. The lipid contents reported here are among the high reported in the literature, note that already under full growth conditions significant lipid levels occurred in the algal enrichment culture. The high lipid productivity of the reported mixed microalgae culture provides an efficient route for efficient algal biodiesel production.

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Autotrophic growth and lipid production of Chlorella sorokiniana in lab batch and BIOCOIL photobioreactors: Experiments and modeling

Cited by 30 publications

References 33 publications

Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

Microalgae and cyanobacteria modeling in water resource recovery facilities: A critical review

Effective role of medium supplementation in microalgal lipid accumulation

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