Autotrophic hydrogen photoproduction by operation of carbon-concentrating mechanism in Chlamydomonas reinhardtii under sulfur deprivation condition

Hong, Min Eui; Shin, Ye Sol; Kim, Byung Woo; Sim, Sang Jun

doi:10.1016/j.jbiotec.2016.01.023

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…Inorganic carbon (CO 2 or bicarbonate) plays three roles by three different mechanisms in oxygenic photosynthesis: CO 2 fixation into carbohydrates (Calvin cycle), CCM and Warburg's effect (the role of bicarbonate in maintaining PSII activity). Hong et al (2016) recorded that during H 2 production phase of S deprivation, the concentrations of starch and H 2 in CCM-induced cells were remarkably enhanced by 65.0% and 218.9% compared to that of CCM-uninduced cells, respectively. Warburg's effect is a unique stimulatory role of CO 2 in the Hill reaction (i.e., O 2 evolution) has been studied by Govindjee and his coworkers (reviewed in Shevela et al 2012).…”

Section: Discussionmentioning

confidence: 98%

“…Respiratory or fermentative metabolism of sugars, derived from starch via glycolysis, supplies a significant number of electrons to the PQ-pool via the plastidial type II NAD(P)H dehydrogenase (NDA2) complex and thereby for the expressed hydrogenase, i.e. PSII-independent hydrogen evolution (Mignolet et al 2012;Volgusheva et al 2013;Hong et al 2016). However, when linear electron transport is limited, neither efficient starch degradation nor high hydrogenase activity would result in strong H 2 production (Nagy et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Light and bicarbonate orchestrate photosynthetic oxygen evolution and sulfur re-addition might recover PSII to resume its activity to evolve oxygen. Other than carbon fixation in Calvin-Benson (CB) cycle and carbon concentrating mechanism (CCM) (Hong et al (2016), bicarbonate affects PSII activity via Warburg's effect (Govindjee and coworkers, reviewed in Shevela et al 2012). Light intensity and quality have been studied in the field of hydrogen evolution (e.g., Jurado-Oller et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Tuning photosynthetic oxygen for hydrogen evolution in synergistically integrated, sulfur deprived consortia of Coccomyxa chodatii and Rhodobium gokarnense at dim and high light

2022

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In this work, tuning oxygen tension was targeted to improve hydrogen evolution. To achieve such target, various consortia of the chlorophyte Coccomyxa chodatii with a newly isolated photosynthetic purple non-sulfur bacterium (PNSB) strain Rhodobium gokarnense were set up, sulfur replete/deprived, malate/acetate fed, bicarbonate/sulfur added at dim/high light. C. chodatii and R. gokarnense are newly introduced to biohydrogen studies for the first time. Dim light was applied to avoid the inhibitory drawbacks of photosynthetic oxygen evolution, values of hydrogen are comparable with high light or even more and thus economically feasible to eliminate the costs of artificial illumination. Particularly, the consortium of 2n− (n = 1.9 × 105 cell/ml, sulfur deprived) demonstrated its perfection for the target, i.e., the highest possible cumulative hydrogen. This consortium exhibited negative photosynthesis, i.e., oxygen uptake in the light. Most hydrogen in consortia is from bacterial origin, although algae evolved much more hydrogen than bacteria on per cell basis, but for only one day (the second 24 h), as kinetics revealed. The higher hydrogen in unibacterial culture or consortia results from higher bacterial cell density (20 times). Consortia evolved more hydrogen than their respective separate cultures, further enhanced when bicarbonate and sulfur were supplemented at higher light. The share of algae relatively increased as bicarbonate or sulfur were added at higher light intensity, i.e., PSII activity partially recovered, resulting in a transient autotrophic hydrogen evolution. The addition of acetic acid in mixture with malic acid significantly enhanced the cumulative hydrogen levels, mostly decreased cellular ascorbic acid indicating less oxidative stress and relief of PSII, relative to malic acid alone. Starch, however, decreased, indicating the specificity of acetic acid. Exudates (reducing sugars, amino acids, and soluble proteins) were detected, indicating mutual utilization. Yet, hydrogen evolution is limited; tuning PSII activity remains a target for sustainable hydrogen production.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tuning photosynthetic oxygen for hydrogen evolution in synergistically integrated, sulfur deprived consortia of Coccomyxa chodatii and Rhodobium gokarnense at dim and high light

2022

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“…Microalgae consist of three kinds of hydrogenase, namely, hydrogenase, reversible hydrogenase, and nitrogenase, where hydrogenase involves [FeFe]-hydrogenase and [NiFe]-hydrogenase. [FeFe] hydrogenase is mostly present in green algae such as Chlorella, Chlamydomonas, and Scenedesmus obliquus [201][202][203].…”

Section: Biogasmentioning

confidence: 99%

Current Advancements in Biofuel Production from Microalgae: Harvesting and Conversion Technologies

et al. 2023

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The objective of this review is to interpret the usage of alternative renewable systems that is imperative for future developments on a global industrial scale. Microalgae have appeared as the most realistic source for biofuel generation due to the accumulation of lipids in most of the strains with rapid biomass expansion and greater photosynthetic efficiency than land plants. Microalgal‐derived feedstocks have a broad range of commercial advantages and can be used as promising renewable fuel substitutes with zero net CO2 emission. Important facets of algal‐based biofuel production, growing and harvesting techniques, approaches to thermochemical conversion, synthesis of commodities with added value, and recent breakthroughs in the field of synthetic biology for fuel production are covered.

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“…More specifically, there are photosynthetic system (PSII) dependence H 2 production pathway, nutrient deprivation H 2 production pathway (i.e., lack of sulfur, nitrogen, phosphorus and magnesium in the medium), and anaerobic biohydrogen production pathway under dark fermentation (Saroussi et al, 2017;Wirth et al, 2018;Suga et al, 2019;Li et al, 2020;Wang et al, 2020). It was found that the most effective way of biohydrogen production was sulfur deficiency compared with nitrogen, phosphorus and magnesium deprivation (Volgusheva et al, 2013;Hong et al, 2016). Sulfur deficiency inhibited PSII activity and oxygen production rate.…”

Section: Microalgae Biohydrogen Productionmentioning

confidence: 99%

Microalgae: The Future Supply House of Biohydrogen and Biogas

et al. 2021

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The non-renewable nature of fossil energy and the environmental pollution caused by its use, such as haze, make it very urgent to develop clean and efficient renewable energy. By using microalgae biomass as an alternative raw material energy sources like biohydrogen, methane can be produced through fermentation and photosynthesis. Unlike solar energy, which has the disadvantages of low energy density, instability and difficulty in storage, biohydrogen and biogas are one of the novel ideal energy sources at present. The utilization of microalgae has various attractive prospects in their production due to its cost-effectiveness, renewable biomass and ease of scaling-up technology. This paper discusses the latest microalgae biomass biohydrogen and biogas production technology including integrated biorefinery systems, co-production or mixed production techniques and puts forward the key problems to be solved in the development of microalgae biohydrogen production technology.

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Autotrophic hydrogen photoproduction by operation of carbon-concentrating mechanism in Chlamydomonas reinhardtii under sulfur deprivation condition

Cited by 11 publications

References 24 publications

Tuning photosynthetic oxygen for hydrogen evolution in synergistically integrated, sulfur deprived consortia of Coccomyxa chodatii and Rhodobium gokarnense at dim and high light

Tuning photosynthetic oxygen for hydrogen evolution in synergistically integrated, sulfur deprived consortia of Coccomyxa chodatii and Rhodobium gokarnense at dim and high light

Current Advancements in Biofuel Production from Microalgae: Harvesting and Conversion Technologies

Microalgae: The Future Supply House of Biohydrogen and Biogas

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