Autumnal increase in proportion of toxic <i>Microcystis</i>
 in Lake Taihu depends more on temperature adaptability than on effects of toxigenicity

Hu, Lang; Kong, Renqiu; Xu, Xudong

doi:10.1111/jam.12868

Cited by 8 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are consistent with previous research which found that the season succession of the Microcystis population in Lake Taihu was dominated by a few genotypes despite there being over 20 genotypes have been identified. 59 Therefore, we propose that future efforts to suppress harmful cyanobacterial blooms in Lake Chaohu should focus on specific strains rather than at the species or above-species level.…”

Section: Resultsmentioning

confidence: 99%

CyanoStrainChip: A Novel DNA Microarray Tool for High-Throughput Detection of Environmental Cyanobacteria at the Strain Level

Shu,

Zhao,

Jia

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

Detecting cyanobacteria in environments is an important concern due to their crucial roles in ecosystems, and they can form blooms with the potential to harm humans and nonhuman entities. However, the most widely used methods for high-throughput detection of environmental cyanobacteria, such as 16S rRNA sequencing, typically provide above-species-level resolution, thereby disregarding intraspecific variation. To address this, we developed a novel DNA microarray tool, termed the CyanoStrainChip, that enables strain-level comprehensive profiling of environmental cyanobacteria. The CyanoStrainChip was designed to target 1277 strains; nearly all major groups of cyanobacteria are included by implementing 43,666 genome-wide, strain-specific probes. It demonstrated strong specificity by in vitro mock community experiments. The high correlation (Pearson's R > 0.97) between probe fluorescence intensities and the corresponding DNA amounts (ranging from 1−100 ng) indicated excellent quantitative capability. Consistent cyanobacterial profiles of field samples were observed by both the CyanoStrainChip and next-generation sequencing methods. Furthermore, CyanoStrainChip analysis of surface water samples in Lake Chaohu uncovered a high intraspecific variation of abundance change within the genus Microcystis between different severity levels of cyanobacterial blooms, highlighting two toxic Microcystis strains that are of critical concern for Lake Chaohu harmful blooms suppression. Overall, these results suggest a potential for CyanoStrainChip as a valuable tool for cyanobacterial ecological research and harmful bloom monitoring to supplement existing techniques.

show abstract

Section: Resultsmentioning

confidence: 99%

CyanoStrainChip: A Novel DNA Microarray Tool for High-Throughput Detection of Environmental Cyanobacteria at the Strain Level

Shu,

Zhao,

Jia

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Hu et al . (2015) proposed that the rapid increase and decrease in the proportions of toxic strains in Microcystis populations in Lake Taihu were associated with temperature adaptability rather than toxigenicity. Liu et al .…”

Section: Discussionmentioning

confidence: 99%

“…There are plenty of studies that have focused on the relationships between toxic cyanobacteria and environmental variables. These include studies that have investigated the relationship between toxic blooms and Microcystis species colony sizes (Kurmayer et al ., 2003), nitrate (Yoshida et al ., 2007), NO x and NH 4 (Ha et al ., 2009), phosphorus (Rinta‐Kanto et al ., 2009; Davis et al ., 2010) and water temperature (Joung et al ., 2011; Hu et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

The involvement of α‐proteobacteria Phenylobacterium in maintaining the dominance of toxic Microcystis blooms in Lake Taihu, China

Zuo

Shen

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

Summary Lake Taihu in China has suffered serious harmful cyanobacterial blooms for decades. The algal blooms threaten the ecological sustainability, drinking water safety, and human health. Although the roles of abiotic factors (such as water temperature and nutrient loading) in promoting Microcystis blooms have been well studied, the importance of biotic factors (e.g. bacterial community) in promoting and meditating Microcystis blooms remains unclear. In this study, we investigated the ecological dynamics of bacterial community, the ratio of toxic Microcystis, as well as microcystin in Lake Taihu. High‐throughput 16S rRNA sequencing and principal component analysis (PCA) revealed that the bacteria community compositions (BCCs) clustered into three groups, the partitioning of which corresponded to that of groups according to the toxic profiles (the ratio of toxic Microcystis to total Microcystis, and the microcystin concentrations) of the samples. Further Spearman's correlation network showed that the α‐proteobacteria Phenylobacterium strongly positively correlated with the toxic profiles. Subsequent laboratory chemostats experiments demonstrated that three Phenylobacterium strains promoted the dominance of the toxic Microcystis aeruginosa PCC7806 when co‐culturing with the non‐toxic PCC7806 mcyB− mutant. Taken together, our data suggested that the α‐proteobacteria Phenylobacterium may play a vital role in the maintenance of toxic Microcystis dominance in Lake Taihu.

show abstract

“…Genomic DNA was extracted as described previously ( Hu et al, 2015 ). Briefly, cells were digested with proteinase K (Thermo Fisher Scientific, United States).…”

Section: Methodsmentioning

confidence: 99%

Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium

Xiao

Jiang

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

Epigenetic modifications such as DNA methylation are well known as connected with many important biological processes. Rapid accumulating evidence shows environmental stress can generate particular defense epigenetic changes across generations in eukaryotes. This transgenerational epigenetic inheritance in animals and plants has gained interest over the last years. Cyanobacteria play very crucial role in the earth, and as the primary producer they can adapt to nearly all diverse environments. However, few knowledge about the genome wide epigenetic information such as methylome information in cyanobacteria, especially under any environment stress, was reported so far. In this study we profiled the genome-wide cytosine methylation from a model cyanobacterium Synechocystis sp. PCC 6803, and explored the possibility of transgenerational epigenetic process in this ancient single-celled prokaryote by comparing the DNA methylomes among normal nitrogen medium cultivation, nitrogen starvation for 72 h and nitrogen recovery for about 12 generations. Our results shows that DNA methylation patterns in nitrogen starvation and nitrogen recovery are much more similar with each other, significantly different from that of the normal nitrogen. This study reveals the difference in global DNA methylation pattern of cyanobacteria between normal and nutrient stress conditions and reports the evidence of transgenerational epigenetic process in cyanobacteria. The results of this study may contribute to a better understanding of epigenetic regulation in prokaryotic adaptation to and survive in the ever changing environment.

show abstract

Autumnal increase in proportion of toxic Microcystis in Lake Taihu depends more on temperature adaptability than on effects of toxigenicity

Abstract: This is the first report that the dynamics of toxic/nontoxic Microcystis was investigated in combination with characterizations of multiple isolated strains. The results are useful in understanding Microcystis genotype succession.

Cited by 8 publications

References 19 publications

CyanoStrainChip: A Novel DNA Microarray Tool for High-Throughput Detection of Environmental Cyanobacteria at the Strain Level

CyanoStrainChip: A Novel DNA Microarray Tool for High-Throughput Detection of Environmental Cyanobacteria at the Strain Level

The involvement of α‐proteobacteria Phenylobacterium in maintaining the dominance of toxic Microcystis blooms in Lake Taihu, China

Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium

Contact Info

Product

Resources

About