Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo

Vaddepalli, Prasad; Zeeuw, Thijs de; Strauss, Soeren; Bürstenbinder, Katharina; Liao, Che‐Yang; Ramalho, João J.; Smith, Richard S.; Weijers, Dolf

doi:10.1016/j.cub.2021.09.019

Cited by 35 publications

(22 citation statements)

References 60 publications

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“…Orientations can be computed with respect to the axes of a local coordinate system defined for the organ, along with its associated positional information ( Figure 8E and I , Figure 8—figure supplement 1A ). It is also possible to quantify how close cell divisions are to common division rules proposed in the literature, such as the shortest wall through the center of the cell including local minima ( Figure 8H ; Besson and Dumais, 2011 ; Yoshida et al, 2014 ; Vaddepalli et al, 2021 ) along the PDG ( Hejnowicz, 2014 )⁠, or rules based on network measures ( Jackson et al, 2019 )⁠.…”

Section: Resultsmentioning

confidence: 99%

Using positional information to provide context for biological image analysis with MorphoGraphX 2.0

Strauss

Runions

Lane

et al. 2022

eLife

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Positional information is a central concept in developmental biology. In developing organs, positional information can be idealized as a local coordinate system that arises from morphogen gradients controlled by organizers at key locations. This offers a plausible mechanism for the integration of the molecular networks operating in individual cells into the spatially-coordinated multicellular responses necessary for the organization of emergent forms. Understanding how positional cues guide morphogenesis requires the quantification of gene expression and growth dynamics in the context of their underlying coordinate systems. Here we present recent advances in the MorphoGraphX software (Barbier de Reuille et al., 2015)⁠ that implement a generalized framework to annotate developing organs with local coordinate systems. These coordinate systems introduce an organ-centric spatial context to microscopy data, allowing gene expression and growth to be quantified and compared in the context of the positional information thought to control them.

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Section: Resultsmentioning

confidence: 99%

Using positional information to provide context for biological image analysis with MorphoGraphX 2.0

Strauss

Runions

Lane

et al. 2022

eLife

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“…Frontiers in Plant Science 07 frontiersin.org Luptovčiak et al, 2017;Ovečka et al, 2020). Little is known about the molecular mechanism underlying this process, but recent studies suggested that auxin is deeply involved in determining cell division patterning (Yamaguchi et al, 2013;Marhavý et al, 2016;Huang et al, 2019;Vaddepalli et al, 2021). Huang et al (2019) demonstrated that auxin signaling regulates cell division patterning during lateral root development (Huang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Division of cortical cells is regulated by auxin in Arabidopsis roots

et al. 2022

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The root cortex transports water and nutrients absorbed by the root epidermis into the vasculature and stores substances such as starch, resins, and essential oils. The cortical cells are also deeply involved in determining epidermal cell fate. In Arabidopsis thaliana roots, the cortex is composed of a single cell layer generated by a single round of periclinal division of the cortex/endodermis initials. To further explore cortex development, we traced the development of the cortex by counting cortical cells. Unlike vascular cells, whose number increased during the development of root apical meristem (RAM), the number of cortical cells did not change, indicating that cortical cells do not divide during RAM development. However, auxin-induced cortical cell division, and this finding was confirmed by treatment with the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) and examining transgenic plants harboring CO2::ΔARF5, in which cortical expression of truncated AUXIN RESPONSE FACTOR5 (ΔARF5) induces auxin responses. NPA-induced cortical auxin accumulation and CO2::ΔARF5-mediated cortical auxin response induced anticlinal and periclinal cell divisions, thus increasing the number of cortical cells. These findings reveal a tight link between auxin and cortical cell division, suggesting that auxin is a key player in determining root cortical cell division.

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“…An intermediate situation was observed in the inner apical domain, where the rule accounted for all but the longitudinal radial orientation. Auxin signaling has been suggested as required for cells to escape the default regime of division plane minimization and to control periclinal divisions at the previous (8C-16C) generation of cell divisions (Yoshida et al, 2014), which could involve a modulation of cell geometry by auxin signaling (Vaddepalli et al, 2021). At subsequent generations, it has instead been reported that the first vascular and ground tissue cells divided periclinally along their maximal (longitudinal) extension when the auxin response was impaired by a ARF5/MP mutation or local ARF inhibition (Möller et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Large-scale analysis and computer modeling reveal hidden regularities behind variability of cell division patterns inArabidopsis thalianaembryogenesis

Laruelle

Belcram

Trubuil

et al. 2022

Preprint

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Noise plays a major role in cellular processes and in the development of tissues and organs. Several studies have examined the origin, the integration or the accomodation of noise in gene expression, cell growth and elaboration of organ shape. By contrast, much less is known about variability in cell division plane positioning, its origin and links with cell geometry, and its impact on tissue organization. Taking advantage of the first-stereotyped-then-variable division patterns in the embryo of the model plant Arabidopsis thaliana, we combined 3D imaging and quantitative cell shape and cell lineage analysis together with mathematical and computer modeling to perform a large scale, systematic analysis of variability in division plane orientation. Our results reveal that, paradoxically, variability in cell division patterns of Arabidopsis embryos is accompanied by a progressive reduction of cell shape heterogeneity. The paradox is solved by showing that variability operates within a reduced repertoire of possible division plane orientations that is related to cell geometry. We show that in several domains of the embryo, a recently proposed geometrical division rule recapitulates observed variable patterns, thus suggesting that variable patterns emerge from deterministic principles operating in a variable geometrical context. Our work highlights the importance of emerging patterns in the plant embryo under iterated division principles, but also reveal domains where deviations between rule predictions and experimental observations point to additional regulatory mechanisms.

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Auxin-dependent control of cytoskeleton and cell shape regulates division orientation in the Arabidopsis embryo

Cited by 35 publications

References 60 publications

Using positional information to provide context for biological image analysis with MorphoGraphX 2.0

Using positional information to provide context for biological image analysis with MorphoGraphX 2.0

Division of cortical cells is regulated by auxin in Arabidopsis roots

Large-scale analysis and computer modeling reveal hidden regularities behind variability of cell division patterns inArabidopsis thalianaembryogenesis

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