Auxin depletion in barley plants under high‐temperature conditions represses DNA proliferation in organelles and nuclei via transcriptional alterations

Oshino, Takeshi; Miura, Shota; Kikuchi, Syunsuke; Hamada, Kazuki; Yano, Kentarô; Watanabe, Masao; Higashitani, Akihiro

doi:10.1111/j.1365-3040.2010.02242.x

Cited by 59 publications

(39 citation statements)

References 20 publications

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“…In developing anthers of barley (Hordeum vulgare) and Arabidopsis, heat stress suppresses the expression of auxin biosynthetic genes (Sakata et al, 2010;Oshino et al, 2011). Auxin depletion then represses DNA proliferation in organelles and nuclei (Oshino et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Auxin depletion then represses DNA proliferation in organelles and nuclei (Oshino et al, 2011). Although the study that included Arabidopsis (Sakata et al, 2010) was not designed to test whether reduced growth might also be linked to altered NO production or accumulation, consistent with the results of that study, L-NMMA, which would not necessarily be expected to alter indole-3-acetic acid biosynthesis, was less effective than heat in reducing tree tobacco shoot growth and leaf expansion (Table II), and L-NMMA did not fully inhibit elongation of the primary root (Table II).…”

Section: Discussionmentioning

confidence: 99%

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Heat Reduces Nitric Oxide Production Required for Auxin-Mediated Gene Expression and Fate Determination in Tree Tobacco Guard Cell Protoplasts

et al. 2012

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Tree tobacco (Nicotiana glauca) is an equatorial perennial with a high basal thermotolerance. Cultured tree tobacco guard cell protoplasts (GCPs) are useful for studying the effects of heat stress on fate-determining hormonal signaling. At lower temperatures (32°C or less), exogenous auxin (1-naphthalene acetic acid) and cytokinin (6-benzylaminopurine) cause GCPs to expand 20-to 30-fold, regenerate cell walls, dedifferentiate, reenter the cell cycle, and divide. At higher temperatures (34°C or greater), GCPs expand only 5-to 6-fold; they do not regenerate walls, dedifferentiate, reenter the cell cycle, or divide. Heat (38°C) suppresses activation of the BA auxin-responsive transgene promoter in tree tobacco GCPs, suggesting that inhibition of cell expansion and cell cycle reentry at high temperatures is due to suppressed auxin signaling. Nitric oxide (NO) has been implicated in auxin signaling in other plant systems. Here, we show that heat inhibits NO accumulation by GCPs and that L-N G -monomethyl arginine, an inhibitor of NO production in animals and plants, mimics the effects of heat by limiting cell expansion and preventing cell wall regeneration; inhibiting cell cycle reentry, dedifferentiation, and cell division; and suppressing activation of the BA auxin-responsive promoter. We also show that heat and L-N G -monomethyl arginine reduce the mitotic indices of primary root meristems and inhibit lateral root elongation similarly. These data link reduced NO levels to suppressed auxin signaling in heat-stressed cells and seedlings of thermotolerant plants and suggest that even plants that have evolved to withstand sustained high temperatures may still be negatively impacted by heat stress.The three major interrelated abiotic plant stresses accompanying global climate change are increasing concentrations of atmospheric CO 2 , heat, and drought (Mittler,

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heat Reduces Nitric Oxide Production Required for Auxin-Mediated Gene Expression and Fate Determination in Tree Tobacco Guard Cell Protoplasts

et al. 2012

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“…Notably, failure during meiosis occurs when temperatures are elevated above those that induce abortion of uninucleate microspores in the same species (T Sage, unpublished observations). In barley, the arrest in anther cell division during meiosis occurs after increasing both day and night temperatures 10 • C at the time of panicle initiation to 30/25 • C for 10 days (Oshino et al, 2007(Oshino et al, , 2011. Cell division arrest in barley anthers was associated with alterations in auxin biosynthesis and a down-regulation of genes encoding DNA replication licensing factors, DNA polymerase, histones, 60S ribosomal proteins, and DEAD/DEAH box RNA helicases (Oshino et al, 2007(Oshino et al, , 2011.…”

Section: High Temperature and Meiosismentioning

confidence: 97%

“…In barley, the arrest in anther cell division during meiosis occurs after increasing both day and night temperatures 10 • C at the time of panicle initiation to 30/25 • C for 10 days (Oshino et al, 2007(Oshino et al, , 2011. Cell division arrest in barley anthers was associated with alterations in auxin biosynthesis and a down-regulation of genes encoding DNA replication licensing factors, DNA polymerase, histones, 60S ribosomal proteins, and DEAD/DEAH box RNA helicases (Oshino et al, 2007(Oshino et al, , 2011. The same HT regime was posited to also result in premature progression to meiotic prophase of microspore mother cells and an early tapetal death (Oshino et al, 2007).…”

Section: High Temperature and Meiosismentioning

confidence: 99%

The effect of high temperature stress on male and female reproduction in plants

Sage

Bagha

Lundsgaard-Nielsen

et al. 2015

Field Crops Research

135

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“…The study suggested that a slight rise in ambient temperature beyond the optimum laboratory conditions triggers flowering of Arabidopsis in the absence of photoperiodic cues which is in turn governed by a set of genes (Balasubhramanian et al 2006). While exposing barley seedlings to high temperature, many of the genes were found to be upregulated during the seedling stage rather than the panicle stage (Oshino et al 2011). A similar study in wheat revealed that the altered gene expression was more during short-term stress rather than prolonged heat stress (Qin et al 2008).…”

Section: Microarraymentioning

confidence: 92%

Transcriptomics of Heat Stress in Plants

Sailaja

Mangrauthia

Neelamraju

et al. 2014

Improvement of Crops in the Era of Climatic Changes

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High-temperature stress is a major abiotic stress that affects various biological processes of plants such as biochemical and physiological response, growth, development, and yield. High-temperature stress has critical effects at cellular and molecular levels also. The increased concentration of regulatory proteins such as heat shock transcription factors (Hsfs) is a major molecular response that occurs during heat stress. These regulatory proteins in turn regulate the expression of heat shock protein (HSP) genes that act as critical players during stress to maintain cell homeostasis. Besides HSPs, the other metabolic and regulatory genes, signaling compounds, compatible osmolytes, and antioxidants too play an important role during heat stress in plants. Apart from the protein-coding genes, recent studies have shown that noncoding microRNAs (miRNAs) also play a key role during heat stress by modulating the gene expression at the transcription and post-transcriptional level. The transcriptome approaches are important to understand the molecular and cellular changes occurring in response to heat stress. The approaches rely mostly by adopting the traditional methods like Northern blot/RNA blot and reverse transcription PCR (RT-PCR), where the expression of the genes can be studied in different tissues and cells, whereas the extent of their expression can be achieved by quantitative PCR or real time PCR. Further, the genome-wide expression profiling tools such as microarray analysis, next-generation sequencing, and RNA sequencing offer a great potential in this direction. This chapter primarily provides the current understanding on the role of regulatory genes (transcription factors), HSP genes, metabolic genes, signaling compounds, osmolytes, reactive oxygen species, 50 B. Sailaja et al.and miRNAs as well as other small RNAs of plants under high temperature. In addition, it gives a brief account of various transcriptome approaches to study the expression profiling of genes during heat stress.

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Auxin depletion in barley plants under high‐temperature conditions represses DNA proliferation in organelles and nuclei via transcriptional alterations

Cited by 59 publications

References 20 publications

Heat Reduces Nitric Oxide Production Required for Auxin-Mediated Gene Expression and Fate Determination in Tree Tobacco Guard Cell Protoplasts

Heat Reduces Nitric Oxide Production Required for Auxin-Mediated Gene Expression and Fate Determination in Tree Tobacco Guard Cell Protoplasts

The effect of high temperature stress on male and female reproduction in plants

Transcriptomics of Heat Stress in Plants

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