Auxin-jasmonate crosstalk in Oryza sativa L. root system formation after cadmium and/or arsenic exposure

Ronzan, Marilena; Piacentini, Diego; Fattorini, Lorenzo; Federica, Della Rovere; Caboni, E.; Eiche, Elisabeth; Ziegler, Jörg; Hause, Bettina; Riemann, Michael; Betti, Camilla; Altamura, Maria Maddalena; Falasca, Giuseppina

doi:10.1016/j.envexpbot.2019.05.013

Cited by 45 publications

(26 citation statements)

References 45 publications

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“…The first point is in contrast with a wide part of literature showing that these pollutants decrease auxin levels (Hu et al, 2013;Srivastava et al, 2013); however, the reverse is also possible. For example, Cd and As increase auxin biosynthesis and accumulation in rice roots in the time interval from two to ten days of seedlings grown (Ronzan et al, 2019) and Cd in the root-forming hypocotyl of Arabidopsis seedlings (Fattorini et al, 2017a). About the second point, the failure of LR formation by high endogenous auxin levels caused by the pollutants and/or a too high exogenous auxin input (Supplementary Figure S1) may be related to the observed altered cellular distribution and influx of auxin by AUX1 in the ARs (Figures 8 and 9).…”

Section: Auxin As a Common Focus Of Both No Mitigating-action And Polmentioning

confidence: 99%

Nitric Oxide Cooperates With Auxin to Mitigate the Alterations in the Root System Caused by Cadmium and Arsenic

et al. 2020

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Oryza sativa L. is a worldwide food-crop frequently growing in cadmium (Cd)/arsenic (As) polluted soils, with its root-system as the first target of the pollutants. Root-system development involves the establishment of optimal indole-3-acetic acid (IAA) levels, also requiring the conversion of the IAA natural precursor indole-3-butyric acid (IBA) into IAA, causing nitric oxide (NO) formation. Nitric oxide is a stress-signaling molecule. In rice, a negative interaction of Cd or As with endogenous auxin has been demonstrated, as some NO protective effects. However, a synergism between the natural auxins (IAA and/or IBA) and NO was not yet determined and might be important for ameliorating rice metal(oid)tolerance. With this aim, the stress caused by Cd/As toxicity in the root cells and the possible recovery by either NO or auxins (IAA/IBA) were evaluated after Cd or As (arsenate) exposure, combined or not with the NO-donor compound sodiumnitroprusside (SNP). Root fresh weight, membrane electrolyte leakage, and H 2 O 2 production were also measured. Moreover, endogenous IAA/IBA contents, transcription-levels of OsYUCCA1 and OsASA2 IAA-biosynthetic-genes, and expression of the IAA-influx-carrier OsAUX1 and the IAA-responsive DR5::GUS construct were analyzed, and NO-epifluorescence levels were measured. Results showed that membrane injury by enhanced electrolyte leakage occurred under both pollutants and was reduced by the treatment with SNP only in Cd-presence. By contrast, no membrane injury was caused by either exogenous NO or IAA or IBA. Cd-and Astoxicity also resulted into a decreased root fresh weight, mitigated by the combination of each pollutant with either IAA or IBA. Cd and As decreased the endogenous NO-content, increased H 2 O 2 formation, and altered auxin biosynthesis, levels and distribution in both adventitious (ARs) and mainly lateral roots (LRs). The SNP-formed NO counteracted the pollutants' effects on auxin distribution/levels, reduced H 2 O 2 formation in Cd-presence, and enhanced AUX1-expression, mainly in As-presence. Each exogenous auxin, but mainly IBA, combined with Cd or As at 10 µM, mitigated the pollutants' effects by increasing LR-production and by increasing NO-content in the case of Cd. Altogether,

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Section: Auxin As a Common Focus Of Both No Mitigating-action And Polmentioning

confidence: 99%

Nitric Oxide Cooperates With Auxin to Mitigate the Alterations in the Root System Caused by Cadmium and Arsenic

et al. 2020

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“…Detailed studies on the effect of As on hormonal crosstalk in plants are scant. The effect of As and/or cadmium on auxin/jasmonate interaction was studied in rice roots [55]. Jasmonates interact with auxin, affecting its homeostasis, during the development of roots in the presence of As and/or Cd stress [55].…”

Section: Plos Onementioning

confidence: 99%

“…The effect of As and/or cadmium on auxin/jasmonate interaction was studied in rice roots [55]. Jasmonates interact with auxin, affecting its homeostasis, during the development of roots in the presence of As and/or Cd stress [55]. The crosstalk between the concentrations of indole-3-acetic acid and bCKs was found in non-stressed Ps and Pc-P, while no significant correlations were determined among growth hormones in the fronds of Pc-A [8].…”

Section: Plos Onementioning

confidence: 99%

Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress

et al. 2020

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Given the close relationship between cytokinins (CKs), photosynthesis and nitrogen metabolism, this study assessed the effect of arsenic (As) contamination on these metabolic components in the As-hyperaccumulators Pteris cretica L. var. Albo-lineata (Pc-A) and var. Parkerii (Pc-P) as well as the As-non-hyperaccumulator Pteris straminea Mett. ex Baker (Ps). The ferns were cultivated in a pot experiment for 23 weeks in soil spiked with As at the levels 20 and 100 mg�kg-1. For the purpose of this study, the CKs were placed into five functionally different groups according to their structure and physiological roles: bioactive forms (bCKs; CK free bases); inactive or weakly active forms (dCKs; CK N-glucosides); transport forms (tCKs; CK ribosides); storage forms (sCKs; O-glucosides); and primary products of CK biosynthesis (ppbCKs; CK nucleotides). An important finding was higher CKs total content, accumulation of sCKs and reduction of dCKs in As-hyperaccumulators in contrast to non-hyperaccumulator ferns. A significant depletion of C resources was confirmed in ferns, especially Ps, which was determined by measuring the photosynthetic rate and chlorophyll fluorescence. A fluorescence decrease signified a reduction in the C/N ratio, inducing an increase of bioactive CKs forms in Pc-P and Ps. The impact of As on N utilization was significant in As-hyperaccumulators. The glutamic acid/glutamine ratio, an indicator of primary N assimilation, diminished in all ferns with increased As level in the soil. In conclusion, the results indicate a large phenotypic diversity of Pteris species to As and suggest that the CKs composition and the glutamic acid/glutamine ratio can be used as a tool to diagnose As stress in plants.

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“…On the contrary, at a concentration of 50 µM or lower, often combined with abiotic stress treatments, elevation of the net photosynthetic rate or photosynthetic pigments were observed [ 96 , 97 ]. It is well known that the level of phytohormones including jasmonic acid varies according to the tissue type, development stage and external stimuli [ 36 , 98 , 99 , 100 , 101 ]. The highest levels of JA have been reported in the fruit, flowers and young leaves, whereas much lower levels have been found in the roots and mature leaves [ 98 , 99 , 102 ].…”

Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley

Kurowska

Daszkowska‐Golec

Gajecka

et al. 2020

IJMS

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Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2, HvTIP2;2, HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action.

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Auxin-jasmonate crosstalk in Oryza sativa L. root system formation after cadmium and/or arsenic exposure

Cited by 45 publications

References 45 publications

Nitric Oxide Cooperates With Auxin to Mitigate the Alterations in the Root System Caused by Cadmium and Arsenic

Nitric Oxide Cooperates With Auxin to Mitigate the Alterations in the Root System Caused by Cadmium and Arsenic

Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley

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