Auxin Levels Regulate the Expression of a Wound-Inducible Proteinase Inhibitor II-Chloramphenicol Acetyl Transferase Gene Fusion in Vitro and in Vivo

Wound Signaling in Tomato Plants1

“…3). These results are consistent with reports that show ABA does not strongly induce Inh I and II genes in tomato (Ryan, 1974;Schaller and Ryan, 1995) and tobacco (Kernan and Thornberg, 1989;Rickauer et al, 1992).…”

“…Auxin was shown to inhibit the activation of an Inh II-CAT chimeric gene (Kernan and Thornberg, 1989) in tobacco calli, but the physiological significance of the inhibition is not known. Ethylene alone does not induce proteinase-inhibitor genes (Ryan, 1974;Kernan and Thornberg, 1989) or other wound-inducible genes (Paradies et al, 1980;Mauch et al, 1984), but recent reports suggest that wound-induced ethylene production is required for maximal expression of defense genes (Weiss and Bevan, 1991;O'Donnell et al, 1996) and requires the presence of JA (Xu et al, 1994;O'Donnell et al, 1996).…”

“…Additionally, it has been reported that ABA-treated tobacco calli (Kernan and Thornberg, 1989) and suspension cells (Rickauer et al, 1992) do not accumulate proteinase inhibitors.…”

Differential Expression of Two Soybean (Glycine max L.) Proline-Rich Protein Genes after Wounding

“…This is supported by recent studies showing that wounding results in a decrease in the levels of free IAA in leaves (Thornburg and Li, 1991). This decrease in auxin concentration has been implicated in the regulation of the proteinase inhibitor genes in tobacco after physical damage (Kernan and Thornburg, 1989). The results reported here indicate that the unique patterns of SbPRPl and SbPRP2 expression in wounded soybean seedlings and leaves reflect alternative defense strategies that have evolved in these tissues and suggest that these mechanisms may be built upon the distinct modes of developmental regulation observed for these two cell wall protein genes.…”

“…A variety of biochemical compounds have been implicated in the regulation of wound-induced genes in plants including oligouronides (Ryan and Farmer, 1991), Sue Ryan, 1990), ethylene (Ecker andDavis, 1987), ABA (Pena-Cortez et al, 1989), methyl jasmonate (Farmer and Ryan, 1990;Creelman et al, 1992), and auxin (Kernan and Thornburg, 1989;Ebener et al, 1993). Whereas ethylene and oligouronides have both been implicated in controlling the localized expression of several wound-induced genes, the expression of PRPs in wounded carrot roots and bean suspension-cultured cells has been shown to be down regulated in the presence of ethylene or fungal elicitors, respectively (Tierney et al, 1988;Sheng et al, 1991), indicating that these compounds are unlikely to be responsible for the accumulation of PRPs after physical damage.…”