Auxin-mediated regulation of vascular patterning in Arabidopsis thaliana leaves

Biedroń, Magdalena; Banasiak, Alicja

doi:10.1007/s00299-018-2319-0

Cited by 40 publications

(26 citation statements)

References 106 publications

(304 reference statements)

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“…In concordance with this, Multani has also observed the cellular architecture of the br2 internodes, and their data showed that the VB numbers of 1th over-ground internode (6-week-old) are reduced [32]; at the same time, Xing found that the VB numbers of 6th over-ground internode (19-leaf stage) are altered (reduced without specific statistics) in br2 mutants [33], which indicated that less-elongated internodes in br2 have reduced VB numbers. Some studies have shown that cell division or differentiation dependent on auxin transport flow is crucial for the formation of vascular bundles in the leaf [62]. Therefore, we speculate that a low auxin level is indispensable for intercalary meristem functions and, in reverse, internode elongation is suppressed when excessive auxin is arrested in intercalary meristem region.…”

Section: Discussionmentioning

confidence: 81%

Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region

et al. 2019

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BackgroundShort internodes contribute to plant dwarfism, which is exceedingly beneficial for crop production. However, the underlying mechanisms of internode elongation are complicated and have been not fully understood.ResultsHere, we report a maize dwarf mutant, dwarf2014 (d2014), which displays shortened lower internodes. Map-based cloning revealed that the d2014 gene is a novel br2 allele with a splicing variation, resulting in a higher expression of BR2-T02 instead of normal BR2-T01. Then, we found that the internode elongation in d2014/br2 exhibited a pattern of inhibition-normality-inhibition (transient for the ear-internode), correspondingly, at the 6-leaf, 12-leaf and 14-leaf stages. Indeed, BR2 encodes a P-glycoprotein1 (PGP1) protein that functions in auxin efflux, and our in situ hybridization assay showed that BR2 was mainly expressed in vascular bundles of the node and internode. Furthermore, significantly higher auxin concentration was detected in the stem apex of d2014 at the 6-leaf stage and strictly in the node region for the ear-internode at the 14-leaf stage. In such context, we propose that BR2/PGP1 transports auxin from node to internode through the vascular bundles, and excessive auxin accumulation in the node (immediately next to the intercalary meristem) region suppresses internode elongation of d2014.ConclusionsThese findings suggest that low auxin levels mediated by BR2/PGP1 in the intercalary meristem region are crucial for internode elongation.

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Section: Discussionmentioning

confidence: 81%

Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region

et al. 2019

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“…Local auxin synthesis by YUCCA flavonoid monooxygenases is essential for cotyledon vasculature development (Cheng et al 2007 ), and the exogenous application of auxin or its transport inhibitors drastically changes leaf venation patterning (Biedroń and Banasiak 2018 ). The drnl cotyledon vasculature phenotypes suggest that DRNL contributes to auxin-related patterning and it is known that DRNL integrates genetically into auxin pathways involving PIN1 (Chandler et al 2011a ).…”

Section: Discussionmentioning

confidence: 99%

Clonal sector analysis and cell ablation confirm a function for DORNROESCHEN-LIKE in founder cells and the vasculature in Arabidopsis

Glowa

Comelli

Chandler

et al. 2021

Planta

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Main conclusion Inducible lineage analysis and cell ablation via conditional toxin expression in cells expressing the DORNRÖSCHEN-LIKE transcription factor represent an effective and complementary adjunct to conventional methods of functional gene analysis. Abstract Classical methods of functional gene analysis via mutational and expression studies possess inherent limitations, and therefore, the function of a large proportion of transcription factors remains unknown. We have employed two complementary, indirect methods to obtain functional information for the AP2/ERF transcription factor DORNRÖSCHEN-LIKE (DRNL), which is dynamically expressed in flowers and marks lateral organ founder cells. An inducible, two-component Cre–Lox system was used to express beta-glucuronidase GUS in cells expressing DRNL, to perform a sector analysis that reveals lineages of cells that transiently expressed DRNL throughout plant development. In a complementary approach, an inducible system was used to ablate cells expressing DRNL using diphtheria toxin A chain, to visualise the phenotypic consequences. These complementary analyses demonstrate that DRNL functionally marks founder cells of leaves and floral organs. Clonal sectors also included the vasculature of the leaves and petals, implicating a previously unidentified role for DRNL in provasculature development, which was confirmed in cotyledons by closer analysis of drnl mutants. Our findings demonstrate that inducible gene-specific lineage analysis and cell ablation via conditional toxin expression represent an effective and informative adjunct to conventional methods of functional gene analysis.

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“…WIND1 is involved in de-differentiation and callus formation via the activation of a signalling pathway in which cytokinins participate (26,27,28). Auxins also have the capacity to induce callus formation (25), although in graft establishment their induction of vascular differentiation is likely more important (29,(30)(31)(32)(33)(34). These hormones, which travel in a basipetal direction (i.e., downward from the tip of the scion), move through the stem, transported by PIN-FORMED (PIN) transport proteins (35).…”

Section: Discussionmentioning

confidence: 99%

Histological changes associated with the graft union development in tomato

Frey

Acebes

Encina

et al. 2020

Preprint

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Background. Despite the importance of grafting in agriculture, particularly in horticultural crops such as tomato (Solanum lycopersicum L.), the structural changes that occur during the establishment of a graft are little understood. Using histochemical techniques, the present work examines the progression of the structure of the graft junction in tomato plants over time.Results. At 10 days after grafting, the cell walls of the scion and rootstock in the area of the graft junction were thicker than usual, and undifferentiated cells appeared associated with the pre-existing vascular tissue. New vascular tissue appeared as branches arising from the pre-existing vasculature, as vascular pockets dispersed within the callus, and as the result of the transdifferentiation of parenchyma cells. Areas showing vascular connections between the scion and rootstock were also seen. Adventitious roots appeared on the scion, arising from the pre-existing vasculature. At 20 days, a great deal of vascular tissue was visible, along with large areas showing vascular connection. At 210 days, vestiges of the changes undergone were still visible. However, no adventitious roots persisted. Conclusions. The area of the graft junction undergoes modifications essential for adequate physiological functioning of grafted plant. The cell walls of the adhesion line change during the process. Pre-existing vasculature plays an important role in the appearance of callus tissue, new vascular cells, and adventitious roots. A long time later the tissues maintain vestiges of graft union development.

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Auxin-mediated regulation of vascular patterning in Arabidopsis thaliana leaves

Cited by 40 publications

References 106 publications

Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region

Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region

Clonal sector analysis and cell ablation confirm a function for DORNROESCHEN-LIKE in founder cells and the vasculature in Arabidopsis

Histological changes associated with the graft union development in tomato

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