Auxin signaling module OsSK41‐OsIAA10‐OsARF regulates grain yield traits in rice

Ma, Fuying; Zhang, Fan; Yu, Zhao; Lan, Dengyong; Yan, Peiwen; Wang, Ying; Hu, Zejun; Zhang, Xinwei; Hu, Jian; Niu, Fuan; Liu, Mingyu; He, Shicong; Cui, Jinhao; Yuan, Xinyu; Yan, Ying; Wu, Shujun; Cao, Liming; Bian, Hongwu; Yang, Jinshui; Li, Zhikang; Luo, Xin

doi:10.1111/jipb.13484

Cited by 9 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The auxin signal transduction pathway, encompassing TIR1/AFB co-receptors, Aux/IAA repressors, and ARF transcription factors, is recognized for its regulatory role in grain yield. In rice, the module of OsSK41-OsIAA10-OsARF is known to regulate grain yield [78]; meanwhile, research has shown that the functions of OsTIR1/AFB genes are redundant for yield in rice [79]. Nevertheless, the module's involvement in the grain filling process remains ambiguous.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanisms of the Grain Filling Rate in Foxtail Millet (Setaria italica L.)

Han,

Zhao,

Zhao

et al. 2024

Agronomy

View full text Add to dashboard Cite

The grain filling rate (GFR) plays a crucial role in determining grain yield. However, the regulatory and molecular mechanisms of the grain filling rate (GFR) in foxtail millet remains unclear. In this study, we found that the GFR of ′Changnong No.47′ (CN47) was significantly higher at 14 DAF (days after flowering) and 21 DAF in comparison to ′Changsheng 13′ (CS13). Furthermore, CN47 also exhibited higher a thousand-grain weight and yield than CS13. Therefore, RNA-seq and UHPLC-MS/MS were used to conduct transcriptome and metabolome analyses during two stages of grain filling in both cultivars. Conjoint analysis of transcriptomics and metabolomics was adopted in order to analyze the biological processes and functional genes associated with GFR. The results identified a total of 765 differentially expressed genes (DEGs) and 246 differentially accumulated metabolites (DAMs) at the 14 DAF stage, while at the 21 DAF stage, a total of 908 DEGs and 268 DAMs were identified. The integrated analysis of co-mapped DAMs and DEGs revealed enriched pathways, including flavonoid biosynthesis, plant hormone signal transduction, tyrosine metabolism, ATP-binding cassette (ABC) transporters, and beta-Alanine metabolism, as well as stilbenoid, diarylheptanoid, and gingerol biosynthesis. In order to elucidate their potential functions in the context of GFR, we developed a gene–metabolite regulatory network for these metabolic pathways. Notably, we found that some genes associated with ABC transporters and the plant hormone signal transduction pathway were implicated in auxin transport and signal transduction, highlighting the crucial role of auxin during grain filling. These findings provide initial insights into the regulatory and molecular mechanisms underlying GFR in foxtail millet, as well as offering valuable genetic resources for further elucidation of GFR in future studies. The findings have also established a theoretical basis for improving the efficiency of yield breeding in foxtail millet.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanisms of the Grain Filling Rate in Foxtail Millet (Setaria italica L.)

Han,

Zhao,

Zhao

et al. 2024

Agronomy

View full text Add to dashboard Cite

show abstract

“…The auxin signal transduction pathway, encompassing TIR1/AFB co-receptors, Aux/IAA repressors, and ARF transcription factors, is recognized for its regulatory role in grain yield. Nevertheless, its involvement in the grain filling process remains ambiguous [78,79]. SAUR genes, which are early auxin-responsive genes, play a role in plant growth, particularly in cell elongation [80].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanism of Grain Filling Rate in Foxtail Millet (<em>Setaria italica L.</em>)

Han,

Zhao,

Zhao

et al. 2024

Preprint

View full text Add to dashboard Cite

The grain filling rate (GFR) plays a crucial role in determining grain yield. However, the regulatory and molecular mechanism of grain filling rate (GFR) in foxtail millet remains unclear. In this study, we found the GFR of 'Changnong No.47' (CN47) was significantly higher at 14 days after flowering (DAF) and 21 DAF in comparison to 'Changsheng 13' (CS13). Furthermore, CN47 also exhibited higher thousand grain weight and yield than CS13. Therefore, we conducted transcriptomics and metabolomics to analyze the biological processes and functional genes associated with GFR during two stages of grain filling in both cultivars. A total of 765 differentially expressed genes (DEGs) and 246 differentially accumulated metabolites (DAMs) were identified at the 14 DAF stage, while at the 21 DAF stage, a total of 908 DEGs and 268 DAMs were identified. The integrated analysis of co-mapped DAMs and DEGs revealed enriched pathways, including flavonoid biosynthesis, plant hormone signal transduction, tyrosine metabolism, ATP-binding cassette (ABC) transporters, beta-alanine metabolism, as well as stilbenoid, diarylheptanoid and gingerol biosynthesis. In order to elucidate their potential functions in the context of GFR, we developed a gene-metabolite regulatory network for these metabolic pathways. Notably we found some genes associated with ABC transporters and plant hormone signal transduction pathway were implicated in auxin transport and signal transduction, highlighting the crucial role of auxin during grain filling. The results provide initial insights into the regulatory and molecular mechanisms underlying GFR in foxtail millet, as well as offer valuable genetic resources for further elucidation of GFR in future studies. The findings have established a theoretical basis for improving the efficiency of yield breeding in foxtail millet.

show abstract

Ubiquitin-Conjugated Enzyme OsUBC16 Negatively Regulates Grain Size and Grain Weight in Rice

Wei,

Zhang,

Yuan

et al. 2024

J. Plant Biol.

View full text Add to dashboard Cite

Auxin signaling module OsSK41‐OsIAA10‐OsARF regulates grain yield traits in rice

Cited by 9 publications

References 37 publications

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanisms of the Grain Filling Rate in Foxtail Millet (Setaria italica L.)

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanisms of the Grain Filling Rate in Foxtail Millet (Setaria italica L.)

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanism of Grain Filling Rate in Foxtail Millet (<em>Setaria italica L.</em>)

Ubiquitin-Conjugated Enzyme OsUBC16 Negatively Regulates Grain Size and Grain Weight in Rice

Contact Info

Product

Resources

About