Availability of biotin incorporated in electrospun PLA fibers for streptavidin binding

Li, Dapeng; Frey, Margaret W.; Vynias, Dionysios; Baeumner, Antje J.

doi:10.1016/j.polymer.2007.08.027

Cited by 35 publications

(43 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of PLA-T6 oligomers should enable a good blending between the P(L)LA matrix and the additive. Moreover, it has been reported that, during fibre formation, the charge applied to the jet promotes the migration of the polarizable species contained in the solution (PLA-T6 in this case) towards the surface of the fibre [118,119].…”

Section: Surface Functionalizationmentioning

confidence: 89%

See 1 more Smart Citation

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

View full text Add to dashboard Cite

and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

show abstract

Section: Surface Functionalizationmentioning

confidence: 89%

“…Since then gas foaming has become an appealing technique for fabricating microporous scaffolds [118,119].…”

Section: Gas Foamingmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

View full text Add to dashboard Cite

show abstract

“…Moreover, the content of nitrogen was different in the different functionalized membranes, and the peak for sulfur of the biotin at 167 eV can be seen in Figure (d) [34] . It meant that the surface-activation and 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 9 functionalization with cyanuric chloride, 1, 3-propanediamine and biotin were successfully carried out and 1, 3-propanediamine-functionalized and biotin-functionalized membranes were obtained.…”

Section: The Surface Chemical Structure Of Pva-co-pe Nanofiber Membranesmentioning

confidence: 99%

Immobilization of Firefly Luciferase on PVA-co-PE Nanofibers Membrane as Biosensor for Bioluminescent Detection of ATP

Wang

Zhao

Luo

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The bioluminescent reaction catalyzed by firefly luciferase has become widely established as an outstanding analytical system for assay of adenosine triphosphate (ATP). When in solution, the luciferase is unstable and cannot be reused. The problem can be partially solved by immobilizing the luciferase on solid substrates. The poly(vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibers membrane has abundant active hydroxyl groups on the surface. The PVA-co-PE nanofibers membrane was first activated by cyanuric chloride with triazinyl group. Then the activated PVA-co-PE nanofibers membrane was subsequently reacted with 1,3-propanediamine and biotin. The firefly luciferase was immobilized onto the surface of 1,3-propanediamine- and biotin-functionalized membranes. The surface chemical structure and morphologies of nanofibers membranes were characterized by FTIR-ATR spectra and SEM. The hydrophilicity of membranes was tested by water contact angle measurements. The detection of fluorescence intensity displayed that the firefly-luciferase-immobilized PVA-co-PE nanofibers membranes indicated high catalytic activity and efficiency. Especially, the firefly-luciferase-immobilized nanofiber membrane which was functionalized by biotin can be a promising candidate as biosensor for bioluminescent detection of ATP because of its high detection sensitivity.

show abstract

“…To create a fibre surface suitable for the capture and subsequent identification of specific biohazards, biotin was added during the spinning process (Li et al 2006a(Li et al , 2007. The biotin molecule is widely used in biohazard sensors because it binds easily with streptavidin protein at four places and other biological molecules including RNA, DNA and antibodies can be biotinylated.…”

Section: Tailoring Surfaces For Biorecognitionmentioning

confidence: 99%

Formation and functions of high-surface-area fabrics

Rebovich

Vynias

Frey

2010

International Journal of Fashion Design, Technology and Educati

Self Cite

View full text Add to dashboard Cite

Non-woven fabrics made from fibres with submicron diameters have extremely high surface-to-volume ratios, which can be tailored and used in a variety of applications. In our research laboratories, fabrics have been made by the electrospinning process. The fabrication method can be adjusted to control the pore spacing between fabrics by controlling the density of fibre collection. By combining dissimilar materials including cellulose nanocrystals, nanoclay and biotin within the nano fibres, we have been able to adjust the strength and the surface properties of the fabrics. Fabrics have been created that can selectively capture biohazards from liquids and particles or volatile organic compounds from air.

show abstract

Availability of biotin incorporated in electrospun PLA fibers for streptavidin binding

Cited by 35 publications

References 31 publications

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Immobilization of Firefly Luciferase on PVA-co-PE Nanofibers Membrane as Biosensor for Bioluminescent Detection of ATP

Formation and functions of high-surface-area fabrics

Contact Info

Product

Resources

About