Availability of Label in 3H-Thymidine for DNA Synthesis in Cultured Macrophages

Eriksen, L.; Eriksen, Nora

doi:10.1038/newbio238270a0

Cited by 7 publications

(5 citation statements)

References 3 publications

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“…In some epithelial cells, 13HlTdR may be stored for use at later times, resulting in a delay between the appearance of 13HlTdR in the cells and its incorporation into DNA (Moffat & Pelc, 1966;Rafferty & Gfeller, 1970;Potten, 1971Potten, , 1973. Macrophages also store some I'HITdR derived DNA precursors until they enter the S-phase (Eriksen & Eriksen, 1972). Potten (1973) pointed out that late DNA labelling from the long-lived precursor pool could result in misinterpretations of the fraction of labelled mitosis (FLM) curves generally applied for cell kinetic studies.…”

Section: Factors Influencing the Labelling Of Nuclear Dnamentioning

confidence: 99%

Potential Pitfalls of [³H]Thymidine Techniques to Measure Cell Proliferation

Maurer

1981

Cell Proliferation

103

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Section: Factors Influencing the Labelling Of Nuclear Dnamentioning

confidence: 99%

Potential Pitfalls of [³H]Thymidine Techniques to Measure Cell Proliferation

Maurer

1981

Cell Proliferation

103

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“…at times when the uptake into proliferative cells in the jejunum, for example, had ceased. Eriksen & Eriksen (1972) demonstrated that macrophages apparently store some 3H-TdR derived DNA precursor until they enter s. This long-lived acid soluble pool may persist for up to 5 days. It is incorporated when the cells enter S and is removed by DNase.…”

Section: Introductionmentioning

confidence: 96%

Further Observations on the Late Labelling Associated With Stimulus‐responsive Cells in Skin

Potten

1973

Cell Proliferation

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The presence of a long‐lived DNA precursor pool which may show some specificity for stimulus‐responsive cells has been demonstrated. Autoradiography, biochemical analysis, hydroxyurea sensitivity, and the temporal response all suggest that the late incorporation is into DNA in cells in the basal layer of the skin that respond to stimulation. The effect is observed with various doses of tritiated thymidine and both methyl and 6 labelled material. 125Iododeoxyuridine also shows late labelling in skin. It is believed that the late labelling is readily distinguishable from reutilization and from possible slow utilization of catabolites into molecules other than DNA. Skin of the ear and dorsum shows similar degrees of late incorporation, while tail and foot skin apparently have smaller long‐lived pools. This may indicate smaller stimulus‐responsive cell populations. If tritiated thymidine is given to cells after stimulation there is still some slow or delayed uptake. The distribution of labelled cells in the autoradiographs suggests that the late labelling cells may be associated somehow with pre‐existing labelled cells (cells in S at the time of thymidine administration).

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“…Recent studies have confirmed conclusively that cells cultured in [3H]thymidine incorporate the label specifically into their newly synthesized DNA (Grieder et al 1970;Eriksen and Eriksen 1972;Fakan and Hancock 1974;Shackney and Ford 1974). The present findings also draw support from the studies of Bulmer and Peel (1974) which &howed that the adjacent deciduum selectively failed to incorporate systemically injected [3H]thymidine.…”

Section: Discussionmentioning

confidence: 72%

An Autoradiographic Study of the Implantation of Transferred Mouse Blastocysts

Kaye¹

1977

Aust. Jnl. Of Bio. Sci.

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Mouse blastocysts collected on day 4 were cultured in [3Hlthymidine (0·01 .uCi/ml) for 24 hand transferred to the uteri of pseudopregnant recipients. Autoradiography revealed that when such blastocysts were allowed to develop for 48 h in utero, label was apparent in the nuclei of decidual cells. The experimental conditions were physiological since blastocysts developed into normal offspring when gestation was allowed to proceed in pseudopregnant recipient animals. The transfer of foetal DNA into maternal decidual cells may be of important immunological significance.

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Availability of Label in 3H-Thymidine for DNA Synthesis in Cultured Macrophages

Cited by 7 publications

References 3 publications

Potential Pitfalls of [³H]Thymidine Techniques to Measure Cell Proliferation

Potential Pitfalls of [³H]Thymidine Techniques to Measure Cell Proliferation

Further Observations on the Late Labelling Associated With Stimulus‐responsive Cells in Skin

An Autoradiographic Study of the Implantation of Transferred Mouse Blastocysts

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Availability of Label in 3H-Thymidine for DNA Synthesis in Cultured Macrophages

Cited by 7 publications

References 3 publications

Potential Pitfalls of [3H]Thymidine Techniques to Measure Cell Proliferation

Potential Pitfalls of [3H]Thymidine Techniques to Measure Cell Proliferation

Further Observations on the Late Labelling Associated With Stimulus‐responsive Cells in Skin

An Autoradiographic Study of the Implantation of Transferred Mouse Blastocysts

Contact Info

Product

Resources

About

Potential Pitfalls of [³H]Thymidine Techniques to Measure Cell Proliferation

Potential Pitfalls of [³H]Thymidine Techniques to Measure Cell Proliferation