Objective: To assess safety practices among eye care workers in Nigeria during the coronavirus disease 2019 (COVID-19) pandemic. Methods: This research was a cross-sectional study. A structured electronic questionnaire was distributed among eye care workers (ophthalmologists, optometrists, ophthalmic nurses) across the six geographical zones of Nigeria. Information on socio-demographics, COVID-19 infection, current working conditions and safety practices were obtained. Data analysis was done using Statistical Package for Social Sciences (SPSS Inc. Chicago Illinois, USA) for windows, version 22. Results: A total of 236 eye care workers participated in the study; their mean (±SD) age was 37.13 (±8.141) years, 125 (53%) were females, and 145 (61.4%) worked in a tertiary care hospital. All participants described COVID-19 as a viral disease and 98.3% agreed that the disease can affect the eyes. Outpatient clinic consultation was recorded as the highest (77.7%) service provided during the pandemic. Regarding preventive practices, 95% of eye care workers wore facemasks, 82.4% wore gloves during ophthalmic examination and Hazmat suit was the least used protective device (2.5%). About three-quarters (77.5%) reported feeling unsafe in their working environment and 63.9% were dissatisfied with the personal protective device provided by hospitals to eye care workers. Conclusion: COVID-19 pandemic presented an unprecedented challenge for eye care workers; with the practice of ophthalmology posing a high risk of transmission of the virus. Efforts should be made to provide the required personal protective devices needed for optimum protection of eye care workers in healthcare settings.