Avaliação computacional da compactação da cromatina e de características morfométricas da cabeça de espermatozoides de coelho (Oryctolagus cuniculus)

Kanayama, Cláudio Yudi; Beletti, Marcelo Emı́lio

doi:10.1590/s0102-09352011000100015

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Morphological evaluation of sperm is commonly used in semen analysis. It is an important parameter in the prediction of male fertility, since it reflects on the genetic characteristics of the animal (Arruda et al, ; Kanayama & Beletti, ). The morphological evaluation must be carried out considering the features of the gamete for each species.…”

Section: Introductionmentioning

confidence: 99%

Morphological and morphometric characterization of domestic cat epididymal sperm

Barbosa

Silva

Tabosa

et al. 2019

Reprod Domestic Animals

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Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm2, while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.

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Section: Introductionmentioning

confidence: 99%

Morphological and morphometric characterization of domestic cat epididymal sperm

Barbosa

Silva

Tabosa

et al. 2019

Reprod Domestic Animals

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“…O Azul de toluidina (AT), é outro método utilizado para identificar alterações da cromatina e que permite avaliação concomitante da morfometria espermática (BELETTI, COSTA, 2003;BELETTI, MELLO, 2004, BELETTI et al, 2005 (BELETTI, MELO., 2004;NAVES et al, 2004;BELETTI et al, 2005;SILVA et al, 2008;RODRIGUES et al, 2009;OLIVEIRA et al, 2011;KANAYAMA;BELETTI, 2011;CAMPOS et al, 2013;SOUZA et al, 2018) O uso de análise de imagem assistida por computador é importante, pois elimina a subjetividade inerente a outros métodos, além de permitir um estudo detalhado das alterações da cromatina, ajudando a identificá-las e classificá-las de acordo com a localização (SOUZA et al, 2018).…”

Section: Azul De Toluidinaunclassified

Avaliação da compactação de cromatina durante a maturação epididimária do espermatozoide de touro

Martins¹

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“…Isso possibilita uma análise detalhada do estudo das alterações da cromatina espermática. Vários estudos utilizando análises computacionais de imagens vêm sendo realizado no laboratório de Biologia da Reprodução da UFU a fim de analisar a cromatina espermática (BELETTI et al, 2004;NAVES et al, 2004;BELETTI et al, 2005;SILVA et al, 2008;RODRIGUES et al, 2009;OLIVEIRA et al, 2011;KANAYAMA;CAMPOS et al, 2012;).…”

Section: -Análise Computacionalunclassified

Classificação das alterações cromatínicas de espermatozoides bovinos e sua correlação com a eficiência na produção in vitro de embriões

Hiraiwa¹

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Chromatin alterations, despite not being evaluated in the spermogram routine affect the fertility of bulls. The most used method of chromatin evaluation is the SCSA (Sperm Chromatin Structure Assay). However it is a high method cost. An alternative method is the toluidine blue (TB) after acid hydrolysis associated with computer analysis -this method is presented as an equivalent and affordable tool. In addition to its lower cost, this methodology measures the intensity of decondensation and heterogeneity chromatin. However, it has not yet determined which decondensation and heterogeneity levels interfere with bull fertility. This study aims to define which levels of decondensation and heterogeneity chromatin are acceptable in order not to prejudice the bull fertility and the embryonic development. It also aims to propose a classification of cattle sperm chromatin alteration according to their location, and it also aims to verify if each type of alteration influences the process of fertilization and the early embryonic development. To this, animals semen samples induced to subfertile were used for in vitro production of embryos (IVP). These samples were evaluated using the AT method, and computer analysis. To characterize the semen fertility samples, cleavage ratio were evaluated on the second day and morphologically normal blastocysts in the seventh day of each IVF. With the use of computer image analysis it was possible to make the following classification of chromatin alterations: decondensation of the base of the head (DB), in the basal half (DBH), in scattered areas (SA), in the central axis of the head (DCA) and the entire length of the head (DT). It was used the estimation of Pearson (p<0,05 and it was considered a tendency to 0,1>p>0,05) in order to relate these alterations with IVP efficiency. In relation to cleavage, DCA and DT had a significant negative correlation (p=0,03 and p=0,047, respectively). DB and DBH showed a tendency to a negative correlation (p=0,06 and p=0,07, respectively). In relation to the blastocyst formation, DB showed a tendency to a negative correlation (p=0,09). In addition to this, two abnormality levels were tested in relation to the Heterogeneity (Het) and Decondensation (Des) of chromatin: above 5%, and above 10%. Des presented a significant correlation related to cleavage when used levels above 5% and 10% of decondensation (p=0,03 and p=0,02, respectively), while Het had no significant correlations neither tendencies with the IVP. It was established that the central axis decondensation of the sperm in IVP intensely affects the fertilization process. Other types of alterations may also affects, but with less intensity. DB may affect in blastocyst formation. To evaluate the decondensation, sperm above 5% Des affect the fertilization process. Related to heterogeneity, regardless of the level considered, does not interfere in both parameters evaluated.

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Avaliação computacional da compactação da cromatina e de características morfométricas da cabeça de espermatozoides de coelho (Oryctolagus cuniculus)

Cited by 6 publications

References 18 publications

Morphological and morphometric characterization of domestic cat epididymal sperm

Morphological and morphometric characterization of domestic cat epididymal sperm

Avaliação da compactação de cromatina durante a maturação epididimária do espermatozoide de touro

Classificação das alterações cromatínicas de espermatozoides bovinos e sua correlação com a eficiência na produção in vitro de embriões

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