Avaliação Da Biomassa De Fungos Amazônicos Como Fonte De Lipases Para Biocatálise

Romano, Israel P.; Santos, Vanderlei S. dos; Louzada, Ana Carolina de Lima Paes; Pereira, Raimundo; Carmo, Edson J. do; Mota, Adolfo José da; Barroso, Hiléia dos S.; Itabaiana, Ivaldo; Pereira, José Odair; Filho, Spartaco Astolfi; Zanotto, Sandra Patricía

doi:10.21577/0100-4042.20170470

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…Vol.67: e24220613, 2024 www.scielo.br/babt used. One of them is to evaluate the delipidated biomass, which in the study conducted by Romano and coauthors [30] presented A. flavor-furcations as the most promising microorganism in screening from seven isolates from Amazonian Forest. This mentioned species showed high synthesis activity of ethyl palmitate by transesterification in an organic medium (668.5 mU/g), with a great prospect in the pharmaceutical and biodiesel industries [30].…”

Section: Discussionmentioning

confidence: 99%

Production and Partial Biochemical Characterization of Multiple Forms of Lipases

Zavarise,

Belei Neto,

Gomes

et al. 2024

Braz. arch. biol. technol.

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The total cost of processes that use lipases can be reduced by using the mycelium or not purified crude extract with lipolytic activity. These catalysts can be used without complex purification and can show desirable properties to emerging applications like hydrolysis and biotransformation of vegetable oils. To explore the potential of multiple forms of lipases (produced by the same filamentous fungus species), this work investigated intracellular and extracellular lipases produced by Trichoderma koningii (intracellular lipases were never studied), Penicillium spp. F02 (isolated from industrial grease trap) and Aspergillus niger. For this, batch fermentations were conducted for 96h using olive oil as a carbon source. The submerged culture was interrupted with vacuum filtration to isolate the mycelium and crude extract. The lipolytic activities were determined by the hydrolysis method of olive oil. The best results were obtained for intracellular activity with T. koningii (81.5 U/g of dry biomass) and for extracellular activity with A. niger (10.6 U/mL of crude extract). Both were attained in 96h of fermentation. T. koningii showed an accumulation of intracellular lipases and a depreciated extracellular activity in the initial 24 hours, which can be reasonable indications that there is strong retention of lipases in the mycelium. Statistical analysis within the range of 37°C to 44°C associated with pH from 7.8 to 8.0 favored the activity of lipases from T. koningii. All these findings are promising for HIGHLIGHTS• Multiple forms of lipases can exhibit different properties and applications.• Mycelium-bound lipases can be helpful for the oleochemical industry.• All of the multiple forms of lipase were studied and presented hydrolysis activity.• Partial biochemical characterization of multiple forms of lipases was realized.• Trichoderma koningii was suggested as a good producer of mycelium-bound lipases.

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Section: Discussionmentioning

confidence: 99%

Production and Partial Biochemical Characterization of Multiple Forms of Lipases

Zavarise,

Belei Neto,

Gomes

et al. 2024

Braz. arch. biol. technol.

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“…[61] Aspergillus flavo-furcatis [62] Aspergillus terreus [63,64] Cunninghamella echinulata [65] Geotrichum spp.…”

Section: Speciesmentioning

confidence: 99%

Lipases: Sources of Acquisition, Ways of Production, and Recent Applications

Santos¹,

Hirata²,

Angelotti³

2022

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Enzymes are extensively used in biotechnological processes in several areas of industry. They are sustainable and safe, and their specificity is another characteristic that improves the performance in the process. Among enzymes, lipase is relevant due to the ability to play different roles in the industry and the possibility of collecting them from microbial sources that are found in industrial residues. This can reduce the costs of enzyme production. In relation to that, lipase immobilization is an interesting process that allows the enzymes to be reused and improves enzyme robustness. Among them, the cross-linked enzyme aggregates (CLEAs) methodology is attractive due to its simplicity, low cost (given the absence of support), and greater interaction with the substrate. Thus, in this review, we discussed the potential of lipase. We reviewed the traditional and new sources of obtaining lipases, along with the ways of improving production, activity, and application in the industry.

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Avaliação Da Biomassa De Fungos Amazônicos Como Fonte De Lipases Para Biocatálise

Cited by 2 publications

References 37 publications

Production and Partial Biochemical Characterization of Multiple Forms of Lipases

Production and Partial Biochemical Characterization of Multiple Forms of Lipases

Lipases: Sources of Acquisition, Ways of Production, and Recent Applications

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