Avaliação da expressão gênica relacionada à receptividade endometrial em pacientes submetidos à embolização do mioma uterino

Hilário, Sandro Garcia

doi:10.11606/t.5.2012.tde-09082012-164244

Cited by 1 publication

(1 citation statement)

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“…Degradation and remodeling of the ECM are also key events in human reproduction ( 9 , 10 ). Hilario et al ( 11 ) evaluated gene expression related to endometrial receptivity in patients undergoing AEM, concluding that there was under-expression of the LIF , IL-11 , and Hoxa-11 genes, and that the expression of IL-6, claudin-4, Hoxa-10, estrogen, and progesterone receptors in the endometrium did not change significantly after AEM. Bernardo et al ( 10 ) screened 86 ECM genes and cytokines and found overexpression of some MMPs after AEM.…”

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confidence: 99%

Extracellular matrix metalloproteinase expression in endometrial tissue after arterial embolization of myomas

Chazan¹,

Bonetti²,

Gomes³

et al. 2021

Clinics

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OBJECTIVES: Arterial embolization of myomas (AEM) is controversial because of the changes that occur in the extracellular matrix (ECM) of the endometrium and its effect on gestational success in infertile patients desiring reproductive capability. Therefore, we performed this study on the expression of genes in the ECM of the endometrium, such as those coding metalloproteinases (MMP), before and 6 months after embolization of the uterine arteries. METHODS: Seven women with leiomyomas were evaluated, and MMP3 and MMP10 levels were measured. The women underwent pelvic nuclear magnetic resonance (NMR), examination, and endometrial biopsy between the 20 th and 24 th day of the menstrual cycle, and pre- and post-AEM (after 6 months). For data analysis, the Cq comparative method, also known as the 2-ΔΔCT method, was used to calculate the relative quantities of MMP gene expression among the samples collected. RESULTS: There was a significant decrease by 9.52 times in the expression of MMP3 ( p =0.007), and a non-significant change in the expression of MMP10 ( p =0.22) in post-AEM-treated women than pre-AEM-treated women. CONCLUSIONS: The results suggest that ECM continues to undergo tissue remodeling 6 months after AEM, at least with regard to MMP3 expression, suggesting that AEM affects the ECM for at least 6 months after the procedure.

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