Avaliação da expressão tecidual do gene de reparo MLH1 e dos níveis de dano oxidativo ao DNA em doentes com câncer colorretal

Cordeiro, Adriana Teixeira; Priolli, Denise Gonçalves; Miranda, Daniel Duarte da Conceição; Júnior, Waldemar Bartchewsky; Margarido, Nelson Fontana; Ribeiro, Marcelo Lima

doi:10.1590/s0101-98802009000300004

Cited by 3 publications

(3 citation statements)

References 33 publications

(89 reference statements)

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“…Genetic studies have shown development of sporadic CRC is an evolutionary process that involves a series of sequential mutations or modifications in the expression of genes related to cell cycle 21 . It is already well established that the development of the CRC from the normal mucosa, is mediated by a sequence of mutations of genes controllers of cell cycle (the proliferation, differentiation, apoptosis and DNA repair) 8 , 30 .…”

Section: Introductionmentioning

confidence: 99%

Tissue Expresion of the Genes Mutyh and Ogg1 in Patients With Sporadic Colorectal Cancer

Nascimento

Ribeiro

Magro

et al. 2017

ABCD, arq. bras. cir. dig.

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Background: MTUYH and OGG1 genes have importance in the base excision repair systems of oxidized DNA bases. Modification of the tissue expression of these genes is related to the increased risk of developing colorectal cancer. Aim: To evaluate the tissue expression of MUTYH and OGG1 comparing normal and neoplastic tissues of patients with sporadic colorectal cancer and to correlate it with clinical and histopathological variables. Method: MUTYH and OGG1 tissue expression was quantified by RT-PCR in patients with colorectal cancer and the values were compared in normal and neoplastic tissues. MUTYH and OGG1 expression was measured and normalized to the constitutive 18S gene. The level of expression of both genes was correlated with the variables: age, gender, tumor location, size of the tumor, histological type, degree of cell differentiation, invasion depth in the intestinal wall, angiolymphatic infiltration, lymph node involvement and TNM staging. Results: Was found downregulation of both genes in neoplastic when compared to normal tissue. There was downregulation of the MUTYH in larger tumors and in patients with angiolymphatic invasion. Tumors with more advanced TNM stages (III and IV) presented downregulation of both genes when compared to those with earlier stages (I and II). Conclusion: The MUTYH and OGG1 genes present downregulation in the more advanced stages of colorectal cancer.

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Section: Introductionmentioning

confidence: 99%

Tissue Expresion of the Genes Mutyh and Ogg1 in Patients With Sporadic Colorectal Cancer

Nascimento

Ribeiro

Magro

et al. 2017

ABCD, arq. bras. cir. dig.

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“…Another one suggests that the methylation process can occur simultaneously in different repair systems 3,14,15 . In a previous study, evaluating the same group of patients, we found that the hMLH1 tgene had lower expression in tumor tissue when compared to normal tissue 29 . It is possible that the hMLH1 gene, one of the main components of the MMR DNA repair system, was also less expressed due to hypermethylation of its promoter region.…”

Section: Discussion Discussion Discussion Discussionmentioning

confidence: 73%

Avaliação da expressão do gene MGMT nos tecidos normal e neoplásico de doentes com câncer colorretal

Cordeiro¹,

Silva²,

Júnior³

et al. 2012

Rev. Col. Bras. Cir.

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OBJETIVO: Avaliar a expressão tecidual do gene de reparo MGMT comparando a mucosa cólica normal e neoplásica em doentes com câncer colorretal. MÉTODOS: Foram estudados 44 portadores de adenocarcinoma colorretal confirmado por estudo histopatológico. Foram excluídos doentes suspeitos de pertencerem a famílias com câncer colorretal hereditário (HNPCC e PAF) e os portadores de câncer do reto médio e inferior submetidos a tratamento quimioradioterápico neoadjuvante. A expressão do gene MGMT foi avaliada pela técnica da reação de polimerase em cadeia em tempo real (RT-PCR). A comparação dos resultados encontrados para expressão do gene MGMT entre tecidos normais e neoplásicos foi feita pelo teste t de Student pareado, adotando-se nível de significância de 5% (p <0,05). RESULTADOS: A expressão tecidual do gene MGMT em todos os doentes foi menor no tecido neoplásico quando comparada a do tecido normal (p=0,002). CONCLUSÃO: O gene de reparo MGMT encontra-se menos expresso no tecido neoplásico quando comparados aos tecidos normais em portadores de CCR esporádico.

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“…25 It was also found that MLH1 repair gene expression is higher in normal tissue than in cancer tissue, which demonstrated the importance of this gene in maintaining DNA integrity. 24,26 CONCLUSION TS, COX2, EGFR, MSH6, MLH1 and p53 expression, as measured through immunohistochemical analysis, was not associated with the clinical-pathological factors of the patients with colorectal adenocarcinoma studied, except for MLH1 in some cases. This marker showed a significant difference in expression between patients at stages III and IV and those at stages I to IV of colorectal adenocarcinoma.…”

Section: Discussionmentioning

confidence: 83%

Immunoexpression of TS, p53, COX2, EGFR, MSH6 and MLH1 biomarkers and its correlation with degree of differentiation, tumor staging and prognostic factors in colorectal adenocarcinoma: a retrospective longitudinal study

Batista

Santos

Vital³

et al. 2019

Sao Paulo Med. J.

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BACKGROUND: There are cases of colorectal tumors that, although small, show more aggressive evolution than large tumors. This motivated us to study whether there are any proteins capable of alerting about these changes. The aim here was to correlate the immunoexpression of the TS, p53, COX2, EGFR, MSH6 and MLH1 biomarkers in tumors in patients with colorectal adenocarcinoma, with the degree of cell differentiation, tumor staging and clinical-pathological prognostic factors. DESIGN AND SETTING: Retrospective observational study at a public tertiary-level hospital. METHODS: We analyzed tissue-microarray paraffin blocks of tumor tissues that had been resected from 107 patients. We used Fisher's exact test to study associations between tumor differentiation/staging and the immunoexpression of biomarkers. We also used Kaplan-Meier estimation, the log-rank test and the adjusted Cox regression model to investigate the patients' overall survival (in months) according to biomarkers and disease-free interval. RESULTS: The degree of tumor differentiation and tumor staging were not associated with the biomarkers, except in cases of patients in stages III or IV, in which there was a correlation with MLH1 expression (P = 0.021). Patient survival and disease-free interval were not associated with the biomarkers. CONCLUSION: There were no associations between the degree of tumor differentiation, staging, length of survival or disease-free interval and the immunoexpression of the TS, p53, COX2, EGFR or MSH6 tumor markers. In advanced cases of colorectal adenocarcinoma (stages III and IV), there was a higher percentage of MLH1-negative results.

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Avaliação da expressão tecidual do gene de reparo MLH1 e dos níveis de dano oxidativo ao DNA em doentes com câncer colorretal

Cited by 3 publications

References 33 publications

Tissue Expresion of the Genes Mutyh and Ogg1 in Patients With Sporadic Colorectal Cancer

Tissue Expresion of the Genes Mutyh and Ogg1 in Patients With Sporadic Colorectal Cancer

Avaliação da expressão do gene MGMT nos tecidos normal e neoplásico de doentes com câncer colorretal

Immunoexpression of TS, p53, COX2, EGFR, MSH6 and MLH1 biomarkers and its correlation with degree of differentiation, tumor staging and prognostic factors in colorectal adenocarcinoma: a retrospective longitudinal study

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