Avaliação da limpeza das paredes dos canais radiculares e da permeabilidade dentinária, após o uso da solução de EGTA isoladamente, ou sem associação com tensoativos

Picoli, Fábio

doi:10.11606/t.25.2007.tde-15062007-130717

Cited by 1 publication

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“…O EDTA, por outro lado, contém 10 átomos de carbono, 16 átomos de hidrogênio, 8 átomos de oxigênio e 2 átomos de nitrogênio (C10H16N2O8). Por outro lado, o EDTA tem maior especificidade pelo magnésio (Picoli, 2007).…”

Section: Resultsunclassified

Expressão gênica de BMPR1, BMPR2 e TGFBR1 liberados de discos de dentina humana tratados com diferentes soluções desmineralizantes e adesão das células da papila apical cultivadas

Araujo¹

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Araujo, PDH. Gene expression of BMPR1, BMPR2 and TGFBR1 released from human dentin disks treated with different demineralizing solutions and adhesion of cultured apical papilla cells [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2018. Versão Original. The success of regenerative endodontic procedures depends on the proper disinfection of the root canal, the presence of undifferentiated mesenchymal cells, the release of growth factors that act as signaling molecules for attraction, proliferation, cell differentiation and the presence of a scaffold that provides support for the organization and vascularization of neoformed tissue. Pulp regeneration protocols have used EDTA to remove the smear layer and release growth factors present in the dentin matrix. A few studies have analyzed whether the use of calcium-specific chelating or less aggressive solutions have better results in the release of growth factors and the adhesion of apical papilla cells to the dentin surface. The objective of this study was to evaluate the gene expression of BMPR1, BMPR2 and TGFBR1 released from human dentin disks previously treated with EDTA, EGTA and Citric Acid and the adhesion of apical papilla cells to these disks. Cryopreserved cells from the cell biorepository of the Basic Biology Laboratory of the Department of Dentistry of the Faculty of Dentistry of the University of São Paulo were used. Dentin discs were obtained from the inner root portion of premolars and human third molars that were obtained from the Biobank of the Human Tooth Division (FOUSP). After 1 min immersion in the demineralizing solutions the discs were transferred to plaques and the cells were plated. The cell adhesion was evaluated after 48 hours using the Scanning Electron Microscopy. Gene expression was determined after the periods of 7 and 21 days of cell culture. Statistical analysis was performed by analysis of variance at 1 criterion (ANOVA) followed by Tukey test post, with a significance level of 5%. At 21 days treatment of dentin with EGTA resulted in a significant increase of BMPR1 compared to PBS. No experimental condition altered BMPR2 expression. The expression of TGBR1 at 21 days was significantly increased by pretreatment of the EGTA discs. Although there was no uniform distribution of cells along the discs, cell adhesion was observed in all experimental groups. It is concluded that the type of demineralizing solution interferes with the released amount of the BMPR1 and TGFBR1 growth factors present in the human dentin, not interfering in the adhesion of apical papilla cells to the dentinal surface.

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Section: Resultsunclassified

Expressão gênica de BMPR1, BMPR2 e TGFBR1 liberados de discos de dentina humana tratados com diferentes soluções desmineralizantes e adesão das células da papila apical cultivadas

Araujo¹

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show abstract

Avaliação da limpeza das paredes dos canais radiculares e da permeabilidade dentinária, após o uso da solução de EGTA isoladamente, ou sem associação com tensoativos

Cited by 1 publication

References 29 publications

Expressão gênica de BMPR1, BMPR2 e TGFBR1 liberados de discos de dentina humana tratados com diferentes soluções desmineralizantes e adesão das células da papila apical cultivadas

Expressão gênica de BMPR1, BMPR2 e TGFBR1 liberados de discos de dentina humana tratados com diferentes soluções desmineralizantes e adesão das células da papila apical cultivadas

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