The hydrographic basin must be considered as a basic planning unit, with the preservation of water resources as a primary factor. In an integrated perspective, the different forms of use can contribute to the degradation of water courses. This study aimed to identify the categories of land use and land cover and to evaluate the environmental impacts in the middle course Piraputangas stream watershed, in the municipality of Cáceres, in the state of Mato Grosso. For the methodological procedures, preparation of the use map and land use coverage, the ArcGis software was used, using the vector database from the IBGE portal, available on the Geosciences website. Likewise, field activities were carried out for informal interviews, observation and application of the Rapid River Assessment Protocol (PAR). The Protocol was applied in three transversal. As for the use and land cover in the middle Piraputangas stream watershed, according to the mapping, there were preserved areas of forest formations (33,20%), the Savânica Formation (10,08%), the Campo Alagado and Swamp Area (0,02%) and Campestre Formation (3,59%). Equally, human activities are present, such as planted forests (0,10%), pastures (51,99%), sugarcane cultivation (0,27%) and other temporary crops (0,66%). Section I presented the best preservation conditions, it was evaluated as natural, obtaining 83 points. In Sections II and III, greater changes were recorded in the characteristics of the bed and in the levels of environmental impacts, totaling 47 points in Section II and 50 in Section III, considered an altered index.