Avaliação de procedimentos de preparo de amostra de amendoim in natura para análise de aflatoxinas

Calori‐Domingues, Maria Antonia; Rechdan, Rafael Condino; Moretti, Ademar; Glória, Eduardo Micotti da; Zambello, Ivani Valarini; Corrente, José Eduardo; Dias, Carlos Tadeu dos Santos

doi:10.1590/s0100-40422010000700012

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…According to Calori-Domingues et al (2010), manual homogenization of the sample influences the variability of the coefficient of variation among subsamples from lots of fresh peanut for analysis of aflatoxins.…”

Section: Resultsmentioning

confidence: 99%

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Size of samples and homogenizers during classification of damaged soybeans

Quirino¹,

Resende

Fonseca

et al. 2019

Rev. bras. eng. agríc. ambient.

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Grain quality determination involves important stages such as collection of the representative sample, homogenization, and dilution. The interrelation among sampling, homogenization, and working sample size is essential to the reliability of the information generated. Therefore, this work aimed to analyse the performance of mechanical homogenizers used in the commercialization of grains in Brazil, as a function of the size of the working sample masses during grain classification. The samples were homogenized and diluted in Boerner, 16:1 multichannel splitter, and 4:1 multichannel splitter until reaching masses of 0.025, 0.050, 0.075, 0.100 and 0.125 kg to determine the level of damaged grains. A 3 x 4 x 5 factorial design was used, meaning three treatments relative to homogenizers (Boerner, 16:1 multichannel splitter, and 4:1 multichannel splitter), four dilutions (4, 8, 12 and 16% damaged grains), and five grain sample sizes (0.025, 0.050, 0.075, 0.100 and 0.125 kg) with nine repetitions. The means were compared by Tukey test and to the original means of prepared samples (4, 8, 12, and 16%) by Student’s t-test. Working samples can be utilized with masses between 0.025 and 0.125 kg to classify damaged soybeans grains. The devices Boerner, 16:1 multichannel splitter, and 4:1 multichannel splitter are similar in the reduction and homogenization of soybean samples for different levels of damaged grains and sample sizes.

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Section: Resultsmentioning

confidence: 99%

“…Several scientific studies have used and recommended the Boerner divider for homogenization and dilution of working samples (Doughtie Júnior, 1947;Fonseca, 2002;Al-Mahasneh & Rababah, 2007), sample divider with inverted channels (sample quartering device) and Gamet divider (Fonseca, 2002;Calori-Domingues et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Size of samples and homogenizers during classification of damaged soybeans

Quirino¹,

Resende

Fonseca

et al. 2019

Rev. bras. eng. agríc. ambient.

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show abstract

“…A Kromasil C18 column (4.6 × 150 mm, 5 μm particle size) was used with ultrapure water, methanol, and acetonitrile in the ratio 60:25:15 (v/v/v) as the mobile phase. The flow rate was 1.0 mL min −1 (35 °C) and 20 min run time . The supernatant was diluted in the mobile phase and centrifuged at 1400× g prior to injection.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and antifungal activity of phenolic compounds and their relation to aflatoxin B1 occurrence in soybeans (Glycine max L.)

2019

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BACKGROUND: Soybean is widely used in food formulations; however, few studies on fungal or mycotoxin contamination have been undertaken. Free, conjugated, and bound phenolic compounds, and their antioxidant and antifungal potential, were therefore evaluated together with the occurrence of aflatoxin B 1 (AFB 1 ) in soybeans. RESULTS:The conjugated and bound phenolic soybean extracts were more efficient for the inhibition of the 2,2 diphenyl-1-picryl-hydrazyl (DPPH) radical, the peroxidase enzyme, and the fungal -amylase enzyme. Aflatoxin B 1, detected at low levels (0.96 to 1.67 ng g −1 ), confirmed the protective effect of soybean phenolic compounds against mycotoxigenic contamination. Principal component analysis confirmed that syringic, p-hydroxybenzoic, p-coumaric acids and vanillin were essential for antioxidant and antifungal activities. CONCLUSION: This study presented new insights into the functionality of phenolic compounds in soybeans, regarding their potential to protect the crops naturally against fungal contamination, avoiding aflatoxin production, as attested by the correlations between phenolic compounds and antioxidant mechanisms. Antifungal potential assayThe antifungal potential of free, conjugated, and bound phenolic fractions was determined through the evaluation of commercial fungal -amylase inhibition, following Silva et al. 7 The specific activity of -amylase was expressed as mg starch mg protein −1 min −1 . The inhibitory effect on -amylase was evaluated by calculating the specific activity after the reaction in the presence of phenolic extracts and expressed as percentage -amylase specific inhibition μg −1 phenolic fraction. Relationship between phenolic compounds and biological activityA correlation analysis was performed on the content of phenolic compounds of conjugated and bound phenolic fractions and FC reducing capacity, PO, DPPH and -amylase inhibition

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Avaliação de procedimentos de preparo de amostra de amendoim in natura para análise de aflatoxinas

Cited by 2 publications

References 11 publications

Size of samples and homogenizers during classification of damaged soybeans

Size of samples and homogenizers during classification of damaged soybeans

Antioxidant and antifungal activity of phenolic compounds and their relation to aflatoxin B1 occurrence in soybeans (Glycine max L.)

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