Avaliação do desempenho reprodutivo de um rebanho bovino de corte naturalmente infectado com o BoHV-1, BVDV e Leptospira hardjo

Junqueira, José Ricardo Cecilio; Freitas, Júlio César de; Alfieri, Alice Fernandes; Alfieri, Amauri Alcindo

doi:10.5433/1679-0359.2006v27n3p471

Cited by 14 publications

(8 citation statements)

References 8 publications

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“…This occurrence was lower than the 98% (204/208) observed by Junqueira et al (2006) in female beef cattle (Nelore and Red Angus x Nelore) aged over 24 months from the west of São Paulo state and the 64% (2,261/3,533) related by Brito et al (2010) in female cattle older than 24 months from different regions of the Goiás state. This lower prevalence obtained in the present study can be attributed to the different cutoff points used in the VN technique, and by the fact that the authors of previous studies considered positive animals with Ab titration ≥8 and ≥4, respectively, while in the present study a cutoff point ≥10 was assumed.…”

Section: Resultscontrasting

confidence: 53%

Sensitivity and specificity of indirect ELISA for the detection of antibody titers against BVDV from beef cattle raised in Pará State

Viana¹,

Kzam²,

Monteiro³

et al. 2017

SCA

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The aims of this study were to establish the prevalence of anti-bovine viral diarrhea virus (BVDV) antibodies (Ab) in beef cattle raised in Pará state, to compare the prevalence of seropositive animals to BVDV using a commercial indirect enzyme-linked immunosorbent assay kit (iELISA) and the virus neutralization (VN) test, and finally, to determine the sensitivity (Se) and specificity (Sp) of the iELISA for the detection of anti-BVDV Ab using VN as a gold standard. A total of 400 serum blood samples from Nelore cows aged at least 24 months from five farms in the Pará state from two mesoregions VN) and between the different farms (1, 2, 3, 4, and 5). The statistical analysis was performed with a significance level of 5%. The prevalence of seropositive animals was found to be different (P < 0.0001) using VN (39.25% [157/400]) and iELISA (54.50% [218/400] 25.93% (63/243) of the samples were considered false-positive and 1.27% false-negative (2/157). It was concluded that the BVDV infection is present in beef cattle herds of the state of Para. Based on the speed of execution, ease of handling, and high Se of the iELISA, it is suggested that this assay can be used as a screening test for the detection of anti-BVDV Ab with the aim of eliminating infected animals from large herds of beef cattle.

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Section: Resultscontrasting

confidence: 53%

Sensitivity and specificity of indirect ELISA for the detection of antibody titers against BVDV from beef cattle raised in Pará State

Viana¹,

Kzam²,

Monteiro³

et al. 2017

SCA

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“…The percentages of observed titers (49.41%, 81.30% and 90% respectively) consistent with active infection. High rates of these infections in cattle herds in Brazil are described by other authors (Junqueira et al 2006, Aono et al 2013, Pereira et al 2013) ( Table 2). The studies of the three previously cited authors used the same cutoff point of this study for serological reactions, however herds or sample had different characteristics.…”

Section: Discussionmentioning

confidence: 65%

“…It is noteworthy that the cows were vaccinated twice a year against leptospirosis, and samples were collected from different properties. Junqueira et al (2006) evaluated a herd of beef cattle in São Paulo state, where the reproductive management consisted of artificial insemination and re-mating with bull and found the following prevalence rates: 81.8% for BoHV-1, 98.9% for BVDV and 79.5% for Leptospira sp. In a herd of unvaccinated Girolando cows (n=84), Pereira et al (2013) found seropositivity rates of 100%, 52.4%, and 45.2% for BoHV-1, BVDV and leptospirosis, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Impact of vaccination on the reproductive performance of multiparous Nellore cows

Ferreira¹,

Fernandes

Silva

et al. 2018

Pesq. Vet. Bras.

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Two experiments were conducted to evaluate the impact of two vaccines on the reproductive performance of multiparous beef cows in Mato Grosso do Sul, Midwest Brazil. In Experiment 1,765 calved multiparous Nellore cows were subjected to the same fixed-time artificial insemination (FTAI) protocol and rebred. Ultrasound pregnancy diagnosis was performed 30 and 90 days post-FTAI (DPI). Rates of pregnancy and pregnancy loss were determined for three periods: from 30 to 90 DPI, from 30 DPI to calving, and from 90 DPI to calving. The cows were assigned to three groups with different vaccination protocols - namely, Group VACMULT (n=250): vaccine against bovine herpesvirus 1 (BoHV-1), bovine viral diarrhea virus (BVDV), and leptospirosis; Group VACL (n=245): vaccine against leptospirosis alone; Group NOVAC (n=270): no vaccination. Serum antibody titers for BoHV-1, BVDV, and leptospirosis, measured in 57 cows from each group indicated active infection, suggesting circulation of these pathogens in the herd. No differences in pregnancy rates were observed across groups. Pregnancy loss rates did not differ significantly across groups within any of the periods investigated (30 to 90 DPI, 30 DPI to calving, or 90 DPI to calving). In Experiment 2, two vaccination protocols for each vaccine were investigated. Group VACGEST was vaccinated on day zero of FTAI (D0) and again 30 days post-FTAI (30 DPI). Group VACPREV was vaccinated on D0 and again on the day of insemination (D11). No significant difference was observed between groups, or significant gestational loss in the group that received the second vaccine on the day of insemination. The results revealed that neither vaccine interfered with the reproductive performance of multiparous cows. No differences were observed between vaccination carried out on both D0 and D11 and that performed on both D0 and D30. The performance of the IBR/BVDV/Leptospirosis vaccine on the day of the artificial insemination did not cause adverse effects on the reproductive parameters.

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“…O isolamento do vírus em cultivo de células de origem bovina é o melhor método de diagnóstico, podendo ser identificado pelas técnicas de imunofluorescência ou virusneutralização. A soroneutralização e o ensaio imunoenzimático (ELISA) são utilizados na rotina para detecção de anticorpos contra o BoHV-1 (BARBOSA et al, 2005;JUNQUEIRA et al, 2006;QUINCOZES, 2005;ROCHA et al, 2001). A vacinação para IBR/IPV é utilizada para o controle da enfermidade, embora não impeça a infecção pelo vírus, diminui consideravelmente a incidência e as manifestações clínicas (HALFEN;VIDOR, 2001).…”

Section: Introductionunclassified

Anticorpos Contra O Herpesvírus Bovino Tipo 1, Vírus Da Diarreia Viral Bovina E Vírus Da Leucose Enzoótica Bovina Na Região Da Campanha Do Estado Do Rio Grande Do Sul

et al. 2016

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É fundamental para o manejo sanitário dos rebanhos bovinos de leite e corte o suporte de laboratórios especializados como uma ferramenta auxiliar no diagnóstico preciso de doenças infecciosas. O objetivo deste trabalho foi apresentar os resultados dos exames sorológicos realizados pelo Laboratório de Virologia e Imunologia da Faculdade de Veterinária da Universidade Federal de Pelotas entre os anos de 2009 e 2012, na área de bovinocultura de corte e leite. Um total de 6092 amostras de soro bovino foram submetidas à técnica de soroneutralização para a detecção de anticorpos específicos contra o herpesvírus bovino tipo 1 (BoHV-1) e o vírus da Diarréia Viral Bovina (BVDV), e à técnica de imunodifusão em gel de ágar para detecção de anticorpos específicos contra o vírus da Leucose Enzoótica Bovina (BLV). Os resultados demonstraram que 52,9%, 85,4% e 41,3% das amostras testadas apresentaram anticorpos específicos contra o BoHV-1, BVDV e o BLV, respectivamente. Os Laboratórios Regionais de Diagnóstico são essenciais para o conhecimento da situação epidemiológica dos rebanhos e diagnóstico preciso de enfermidades infecciosas de etiologia viral.

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Avaliação do desempenho reprodutivo de um rebanho bovino de corte naturalmente infectado com o BoHV-1, BVDV e Leptospira hardjo

Cited by 14 publications

References 8 publications

Sensitivity and specificity of indirect ELISA for the detection of antibody titers against BVDV from beef cattle raised in Pará State

Sensitivity and specificity of indirect ELISA for the detection of antibody titers against BVDV from beef cattle raised in Pará State

Impact of vaccination on the reproductive performance of multiparous Nellore cows

Anticorpos Contra O Herpesvírus Bovino Tipo 1, Vírus Da Diarreia Viral Bovina E Vírus Da Leucose Enzoótica Bovina Na Região Da Campanha Do Estado Do Rio Grande Do Sul

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