Avaliação do extrato hidroalcoólico de Mentha crispa sobre a performance reprodutiva em ratos Wistar

Abstract

RESUMO:A Mentha crispa (Lamiaceae) é conhecida popularmente como hortelã-da-folha-miúda. O extrato hidroalcoólico (EHA) de Mentha crispa, obtido do caule e da folha, possui atividade amebicida (Entamoeba histolytica) e giardicida (Giardia lamblia) e é explorado comercialmente na produção do fi toterápico Giamebil plus ® . Os efeitos da administração oral subcrônica do EHA de Mentha crispa foram investigados sobre a performance reprodutiva em ratos Wistar adultos. Três grupos de ratos machos (n=6-12/grupo) foram tratados durante 30 dias consecutivos com EHA por via oral nas doses de 0,5 e 1,0 g/kg de peso ou água destilada (grupo controle). Em seguida, foram determinados os índices reprodutivos, massa dos órgãos e análise morfológica do testículo, epidídimo e ducto deferente. Os resultados mostram que, durante o período de tratamento, não se observaram sinais de toxicidade ou morte dos animais. Os índices reprodutivos, assim como a massa e as morfologias macro e microscópica dos órgãos não foram modifi cados pela administração subcrônica do EHA. Dessa forma, conclui-se que a administração subcrônica do extrato hidroalcoólico de Mentha crispa (Giamebil plus ABSTRACT: "Evaluation of the hydroalcoholic extract of Mentha crispa on the reproductive performance in Wistar rats". Mentha crispa (Lamiaceae) is commonly known as "hortelã-dafolha-miúda". The hydroalcoholic extract (HAE) from the leaves and stem of Mentha crispa possesses amebicidal (Entamoeba histolytica) and giardicidal (Giardia lamblia) activity and it is commercially used in the production of the phytoterapic Giamebil plus ® . The effects of the subchronic administration of the HAE of Mentha crispa were investigated on reproductive performance in adult Wistar rats. Three groups of male rats (n=6-12/group) were orally treated daily for 30 consecutive days with the HAE at doses of 0.5 and 1.0 g/kg or distilled water (control group). Then, the reproductive indices, the organ weight and the morphologic analysis of testicle, epididymis and vas deferens were determined. There were no signs of toxicity or deaths during the period of treatment. Furthermore, the reproductive indices, the organ weight and the macroscopic and microscopic morphologic analysis of the organs were not altered by subchronic administration of the HAE of Mentha crispa. It shows that the subchronic administration of the HAE of Mentha crispa (Giamebil plus ® ) did not possess contraceptive activity on the reproductive system of Wistar rats.

Effects of the oral treatment with Copaifera multijuga oil on reproductive performance of male Wistar rats

“…For microscopic analysis, these organs were fixed in totum in 10% buffered formalin for 48 h at room temperature; afterwards, each sample was washed with water and immersed in 70% ethanol for 3 to 4 days, and then embedded in paraffin. Paraffin sections of 5 μm were obtained and stained with hematoxylin/eosin (HE), and they were subsequently analyzed using the digital image acquisition system MicroDIP/Kacil ® (Dimech et al, 2006).…”

Preliminary assessment of Rosmarinus officinalis toxicity on male Wistar rats' organs and reproductive system

“…The results show that the administration of the lower dose (291.2 mg/kg of body weight) of R. offi cinalis with death occurring at three and 14 days after the end of treatment did not produce any signifi cant weight alteration in the organs of the reproductive system, the accessory glands, liver, kidneys, lungs, brain and pituitary gland (Tables 1 and 2). However, the administration of twice the dose (582.4 mg/kg of body weight) resulted in a signifi cant weight increase of the seminal vesicle of the treated rats whereas the weights of the other organs analyzed were not signifi cantly altered (Table 3) (Dimech et al, 2006).…”

Embryo and fetal toxicity ofMentha x villosaessential oil in Wistar rats

“…The only study found in reproduction was developed with an alcoholic extract. It revealed that the extract was not able to promote fertility impairment and a contraceptive effect in male rats (Dimech et al, 2006). Considering that pregnant women can employ this species as a medicine (tea or essential oil) and as a food spice, the aim of the present study was to elucidate if Mentha x villosa essential oil impairs gestation and whether it is toxic to fetuses exposed during gestation.…”

“…A study developed with an alcoholic extract obtained from leaves of Mentha x villosa showed that male rats pre-treated with 0.5 and 1.0 g/kg of the extract for 30 days did not show fertility impairment nor promoted alterations at gestation and reproductive performance of no treated dams mated with these males after treatment ending period (Dimech et al, 2006). In another study, an aqueous extract from M. arvensis promoted fertility impairment, decreased number and motility of sperm and reduced testis and epididymis size and weight in mice treated for 20 days (Sharma & Jacob 1996, 2001.…”