Avaliação do perfil de expressão de genes relacionados a via de microrna’s e apotose em células neurais infectadas experimentalmente pelo vírus Zika (ZIKV)

Abstract

Virus zika is an arbovirus transmitted by Aedes mosquitoes, occurring described together with the increase in microcephaly in newborns. In view of the severity associated with cases of microcephaly and other neurological disorders, the World Health Organization came to characterize it as a Public Health Emergency of National Importance. The objective of this work was to determine the expression profile of genes that encode key proteins related to microRNA and apoptosis pathways in neural cells of rodents of the Muridae family infected by this virus. The infected neural cells were Neuro-2a, and the methods employed were quantitative reverse transcription polymerase chain reaction and bioluminescence. Viral production and caspase-3/7 activation were higher at 96 hours after infection, as well as the expression of genes encoding Ago1, DGCR8, RIG-I and FAS-L.

Infection by Zika Vírus in human cells alters the expression profile of miRNA-15 and activation of apoptotic caspases

“…The ineffectiveness of public policies in the control of the vector has increased the number of cases of the disease in regions of dominant endemicity, concomitantly associated with the proliferation of the agent. Thus, strategies that promote the search for host immune response mechanisms associated with the biogenesis of molecules that mediate gene expression become more effective, as they were able to establish the physiological basis of the human cells behavior against viral infection (Amorim, et al, 2020;Fecury, et al, 2020). This arbovirus, stood out mainly for its ability to cause mild symptoms such as fever and headache, even the most severe neurological cases (Chambers, et al, 1990).…”

“…in newborns and adults. (Amorim, et al, 2020;Who, 2009;Dick, et al,, 1952;Duffy, Research, Society and Development, v. 9, n. 12, e3991210699, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10. 33448/rsd-v9i12.10699 et al, 2009).…”