Avaliação fenotípica da enzima Klebsiella pneumoniae carbapenemase (KPC) em Enterobacteriaceae de ambiente hospitalar

Dienstmann, Rosabel; Picoli, Simone Ulrich; Meyer, Gabriela; Schenkel, Tiago; Steyer, Juçara Rodrigues

doi:10.1590/s1676-24442010000100005

Cited by 30 publications

(36 citation statements)

References 11 publications

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“…This is a cause for concern because Klebsiella sp. are often resistant to different classes of drugs commonly used in the treatment of infections involving multidrug-resistant Enterobacteriaceae, but show especially high resistance rates to carbapenems (21) (25) .…”

Section: Ethical Considerationsmentioning

confidence: 99%

Occurrence and sensitivity profile of extended spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary hospital in Southern Brazil

Rugini

Sobottka

Fuentefria

2015

Rev. Soc. Bras. Med. Trop.

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Section: Ethical Considerationsmentioning

confidence: 99%

Occurrence and sensitivity profile of extended spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary hospital in Southern Brazil

Rugini

Sobottka

Fuentefria

2015

Rev. Soc. Bras. Med. Trop.

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“…The mechanisms via which Enterobacteriaceae resists different classes of antimicrobials vary; for example, the mechanisms may be associated with the decrease or loss of porin in bacterial outer membranes (OMPs) and efflux pumps, mutations in the active site of antimicrobials that decreases their affinity for microbes, and the presence of β-lactamase-encoding genes 3,[5][6][7] . Among the carbapenemases produced from plasmids, the Ambler class A (Klebsiella pneumoniae carbapenemase -KPC and Guiana-Extended-Spectrum-GES) has been identified in clinical isolates 8 .…”

Section: Introductionmentioning

confidence: 99%

Phenotypic methods for screening carbapenem-resistant Enterobacteriaceae and assessment of their antimicrobial susceptibility profile

Silva

Rampelotto

Lorenzoni

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:In this study, we used phenotypic methods to screen carbapenem-resistant Enterobacteriaceae (CREs) and evaluated their antimicrobial sensitivity profile. Methods: One hundred and seventy-eight CREs were isolated at a university hospital in south Brazil in a one-year period. Samples were assessed using disk diffusion tests with inhibitors of β-lactamases such as phenylboronic acid (AFB), cloxacillin (CLOXA), and ethylenediaminetetraacetic acid (EDTA). Strains with differences in zone diameters ≥ 5mm for disks supplemented or not were considered producers of carbapenemases. Results: Klebsiella pneumoniae was the most prevalent CRE, which appeared in 80.3% cases (n = 143). Among clinical materials, the rectal swab was responsible for 43.4% of the isolations (n = 62), followed by urine (18.9%; n = 27). Among the CREs identified in this study, the growth of 56.7% (n = 101) isolates, which were putative producers of Klebsiella pneumoniae carbapenemase (KPC), were inhibited by AFB, whereas 7.3% (n = 13) isolates were inhibited by both AFB and CLOXA and were considered as putative producers of plasmid-mediated AmpC; approximately 3.4% (n = 6) were inhibited by EDTA, which possibly produced metallo-β-lactamase. Lastly, 32.6% (n = 58) cases showed negative results for AFB, CLOXA, and EDTA sensitivity, and represented another class of β-lactamases and/or mechanism of resistance. Conclusions: Phenotypic screening of CREs is important for clinical laboratories that monitor outbreaks of resistant microbes. Phenotypic tests that use carbapenemase inhibitors and enhancers such as AFB, CLOXA, and EDTA are necessary since they are good screening methods for the detection of carbapenemases.

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“…Sendo a resistência em bactérias originadas de diversas formas, como por exemplo, o uso inadequado de antibióticos. Portanto, a resistência de S. aureus e K. pneumoniae tem se tornado um problema de saúde pública e alvo de atenção em todos os campos da saúde 1,2 . A Staphyloococcus aureus é um microorganismo Gram positivo, membro da família Micrococcaceae.…”

Section: As Bactérias Staphylococcus Aureus Eunclassified

Prospecção antibacteriana de compostos sintéticos contra Staphylococcus aureus e Klebsiella pneumoniae

Miranda

Bacelar

Pereira

et al. 2013

BBR - Biochem. Biotechnol.

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Avaliação fenotípica da enzima Klebsiella pneumoniae carbapenemase (KPC) em Enterobacteriaceae de ambiente hospitalar

Cited by 30 publications

References 11 publications

Occurrence and sensitivity profile of extended spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary hospital in Southern Brazil

Occurrence and sensitivity profile of extended spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary hospital in Southern Brazil

Phenotypic methods for screening carbapenem-resistant Enterobacteriaceae and assessment of their antimicrobial susceptibility profile

Prospecção antibacteriana de compostos sintéticos contra Staphylococcus aureus e Klebsiella pneumoniae

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