AVALIAÇÃO FENOTÍPICA E GENOTÍPICA DO PERFIL DE RESISTÊNCIA DE AMOSTRAS DE Staphylococcus aureus ISOLADAS DE CULTURAS CLÍNICAS E DE VIGILÂNCIA DE UM HOSPITAL DE ENSINO BRASILEIRO

Abstract

Staphylococcus aureuscan cause a variety of infections, especially nosocomial. Its importance lies in the combination of virulence, invasiveness and antibiotic resistance constituting therapeutic challenges. This study aimed to assess the genotype and phenotypic resistance profiles of samples of S. aureusisolated from hospitalized patients in a Brazilian teaching hospital. We evaluated 1078 samples obtained from surveillance cultures and clinical S.aureusin hospitalized patients. To assess the phenotypic resistance profile was used disk diffusion method according to CLSIcriteria, 2011. For the determination of genotypic resistance was the presence of the mecAgene by polymerase chain reaction -PCR. Of the 1078 samples tested phenotypically, it was observed that 75.1% of the samples were Methicillin Resistant Staphylococcus aureus(MRSA), and of these, 98.4% were resistant to oxacillin and 100%, cefoxitin. To determine genotype was performed for the PCR amplification of the mecAgene. Of the 443 samples tested for the mecA336 samples were positive (75.8%). Of these samples, 85.7% showed resistance phenotype oxacillin and cefoxitin 88.4%. Due to the high rate of MRSA, concludes the need for investment in research, rational use of antimicrobials and creation of reference laboratories for verification of antimicrobial resistance