Avian Collagen Is Useful for the Construction of Skin Equivalents

Li, Hailan; Yun, Hye‐Young; Baek, Kwang Jin; Kwon, Nyoun Soo; Choi, Hyunjin; Park, Kyoung-Chan; Kim, Dong-Seok

doi:10.1159/000480659

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…Increased extracellular matrix (ECM) content increased ΔNp63 in corneal epithelium, whilst collagen synthesis inhibition reduced ΔNp63 levels [200]. Altered p63 levels were also noted in keratinocytes grown on different ECM components [201] and ΔNp63 levels in limbal epithelial stem cells vary according to matrix composition and stiffness [202]. Activating the laminin receptor increased ΔNp63 levels in keratinocytes [203] and integrin‐linked kinase (ILK) inhibition reduced ΔNp63 [204].…”

Section: Signalling Pathways That Regulate P63 Levels or Activitymentioning

confidence: 99%

The foggy world(s) of p63 isoform regulation in normal cells and cancer

Pokorná

Vysloužil

Hrabal

et al. 2021

The Journal of Pathology

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The p53 family member p63 exists as two major protein variants (TAp63 and ΔNp63) with distinct expression patterns and functional properties. Whilst downstream target genes of p63 have been studied intensively, how p63 variants are themselves controlled has been relatively neglected. Here, we review advances in understanding ΔNp63 and TAp63 regulation, highlighting their distinct pathways. TAp63 has roles in senescence and metabolism, and in germ cell genome maintenance, where it is activated post‐transcriptionally by phosphorylation cascades after DNA damage. The function and regulation of TAp63 in mesenchymal and haematopoietic cells is less clear but may involve epigenetic control through DNA methylation. ΔNp63 functions to maintain stem/progenitor cells in various epithelia and is overexpressed in squamous and certain other cancers. ΔNp63 is transcriptionally regulated through multiple enhancers in concert with chromatin modifying proteins. Many signalling pathways including growth factors, morphogens, inflammation, and the extracellular matrix influence ΔNp63 levels, with inconsistent results reported. There is also evidence for reciprocal regulation, including ΔNp63 activating its own transcription. ΔNp63 is downregulated during cell differentiation through transcriptional regulation, while post‐transcriptional events cause proteasomal degradation. Throughout the review, we identify knowledge gaps and highlight discordances, providing potential explanations including cell‐context and cell–matrix interactions. Identifying individual p63 variants has roles in differential diagnosis and prognosis, and understanding their regulation suggests clinically approved agents for targeting p63 that may be useful combination therapies for selected cancer patients. © 2021 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

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Section: Signalling Pathways That Regulate P63 Levels or Activitymentioning

confidence: 99%

The foggy world(s) of p63 isoform regulation in normal cells and cancer

Pokorná

Vysloužil

Hrabal

et al. 2021

The Journal of Pathology

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“…To date, collagen remains the most popular ECM-based biomaterial for dermal matrix biofabrication. These collagens are typically derived from animal sources such as bovine collagen ,, or rat tail collagen. − Alternatively, avian collagen, ovine collagen, porcine collagen, , and duck feet collagen are used, albeit to a lesser extent for dermal matrix development. Although human collagen can also be derived from skin biopsies and cadaveric tendons, research into its use in organotypic skin models remain elusive.…”

Section: Ecm-based Organotypic Skin Models Using Conventional Static ...mentioning

confidence: 99%

Advancements in Extracellular Matrix-Based Biomaterials and Biofabrication of 3D Organotypic Skin Models

Phang

Basak

Teh

et al. 2022

ACS Biomater. Sci. Eng.

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Over the last decades, three-dimensional (3D) organotypic skin models have received enormous attention as alternative models to in vivo animal models and in vitro twodimensional assays. To date, most organotypic skin models have an epidermal layer of keratinocytes and a dermal layer of fibroblasts embedded in an extracellular matrix (ECM)-based biomaterial. The ECM provides mechanical support and biochemical signals to the cells. Without advancements in ECM-based biomaterials and biofabrication technologies, it would have been impossible to create organotypic skin models that mimic native human skin. In this review, the use of ECM-based biomaterials in the reconstruction of skin models, as well as the study of complete ECM-based biomaterials, such as fibroblasts-derived ECM and decellularized ECM as a better biomaterial, will be highlighted. We also discuss the benefits and drawbacks of several biofabrication processes used in the fabrication of ECM-based biomaterials, such as conventional static culture, electrospinning, 3D bioprinting, and skin-on-achip. Advancements and future possibilities in modifying ECM-based biomaterials to recreate disease-like skin models will also be highlighted, given the importance of organotypic skin models in disease modeling. Overall, this review provides an overview of the present variety of ECM-based biomaterials and biofabrication technologies available. An enhanced organotypic skin model is expected to be produced in the near future by combining knowledge from previous experiences and current research.

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“…Ca 2+ -influx, mediated by TRPV4, guides various physiological functions, including nociception through pressure-sensing and has a role in the development of functional vascular system 19,24,25 . Additionally, it plays a central role in cell volume regulation by acting as a membrane-stretch-sensing channel upon osmotic changes 26,27 . Besides these physiological functions, TRPV4 seems to be important for the maintenance of epithelial integrity through its role in the regulation of on adherens and tight junction proteins [28][29][30][31][32][33] .…”

mentioning

confidence: 99%

Mechanosensitive TRPV4 channel guides maturation and organization of the bilayered mammary epithelium

Tytti,

Sanna,

Carla

et al. 2024

Sci Rep

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Biophysical cues from the cell microenvironment are detected by mechanosensitive components at the cell surface. Such machineries convert physical information into biochemical signaling cascades within cells, subsequently leading to various cellular responses in a stimulus-dependent manner. At the surface of extracellular environment and cell cytoplasm exist several ion channel families that are activated by mechanical signals to direct intracellular events. One of such channel is formed by transient receptor potential cation channel subfamily V member, TRPV4 that is known to act as a mechanosensor in wide variaty of tissues and control ion-influx in a spatio-temporal way. Here we report that TRPV4 is prominently expressed in the stem/progenitor cell populations of the mammary epithelium and seems important for the lineage-specific differentiation, consequently affecting mechanical features of the mature mammary epithelium. This was evident by the lack of several markers for mature myoepithelial and luminal epithelial cells in TRPV4-depleted cell lines. Interestingly, TRPV4 expression is controlled in a tension-dependent manner and it also impacts differentation process dependently on the stiffness of the microenvironment. Furthermore, such cells in a 3D compartment were disabled to maintain normal mammosphere structures and displayed abnormal lumen formation, size of the structures and disrupted cellular junctions. Mechanosensitive TRPV4 channel therefore act as critical player in the homeostasis of normal mammary epithelium through sensing the physical environment and guiding accordingly differentiation and structural organization of the bilayered mammary epithelium.

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Avian Collagen Is Useful for the Construction of Skin Equivalents

Cited by 6 publications

References 20 publications

The foggy world(s) of p63 isoform regulation in normal cells and cancer

The foggy world(s) of p63 isoform regulation in normal cells and cancer

Advancements in Extracellular Matrix-Based Biomaterials and Biofabrication of 3D Organotypic Skin Models

Mechanosensitive TRPV4 channel guides maturation and organization of the bilayered mammary epithelium

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