Avian egg antibodies: basic and potential applications

Kovacs-Nolan, Jennifer; Mine, Yoshinori

doi:10.3184/147020604783637462

Cited by 72 publications

(36 citation statements)

References 172 publications

(177 reference statements)

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“…The results indicated that anti-NANOS2 IgY antibody showed the better affinity and specific reactivity to the rNANOS2 protein of dairy goat origin than anti-NANOS2 antibodies got from rabbit. It is extensively reported that chicken antibodies could bind to more epitopes on a mammalian protein than the corresponding mammalian antibodies and the absence of immunological cross-reactivity between chicken IgY and mammalian IgG was determined by the evolution divergence (Hadge and Ambrosius 1984;Kovacs-Nolan and Mine 2004;Dias da Silva and Tambourgi 2010). These findings strongly support the application of anti-NANOS2 IgY as a research tool to explore the biological functions of NANOS2 protein in male germ cell development.…”

Section: Controlsupporting

confidence: 59%

“…The phylogenetic distance between mammals and chickens renders more advantage for the preparation of antibodies in chickens against a wide range of highly conserved mammalian proteins (Kovacs-Nolan and Mine 2004). Hence, the present study was aimed at preparing the chicken immunoglobulin Y (IgY) against recombinant NANOS2 RNA binding protein of dairy goat origin and comparing the similarity and difference between the NANOS2 of mice and dairy goat origin using bioinformatics tools to identify the difference in antigenic determinants.…”

Section: Doi: 1017221/8129-cjasmentioning

confidence: 99%

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Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies

et al. 2015

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Available rabbit anti-NANOS2 antibodies failed to recognize the NANOS2 protein of goat origin though they showed better reactivity towards the same protein of human and mice origin. A specific antibody against goat NANOS2 is necessary to explore the biological functions of dairy goat NANOS2 protein. Recombinant NANOS2 protein (dairy goat origin) was expressed in E. coli (BL21). Anti-NANOS2 immunoglobulin Y (IgY; in chicken) was developed and evaluated. NANOS2 protein sequences between mice and dairy goat were compared using bioinformatics tools. The specific IgY titer attained the peak (≥ 1 : 128 000) after the 3 rd booster injection, then it gradually decreased to 1 : 16 000 and remained stable. The specificity and sensitivity of anti-NANOS2 IgY was superior to rabbit anti-NANOS2 IgG. Though, the functional domain 63-116 or 62-115 of NANOS2 protein is highly conserved with 100% identity in dairy goat and mice, the considerable difference in sequence at region 18-61 was identified from the predicted epitopes. According to our survey, this is the first report on preparation of chicken IgY against NANOS2 protein and indicating the prerequisite of species specific NANOS2 antibodies due to epitopes difference in functionally conserved NANOS2 protein. Anti-NANOS2 IgY could be applied as a novel research tool in developmental biology.

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Section: Controlsupporting

confidence: 59%

Section: Doi: 1017221/8129-cjasmentioning

confidence: 99%

Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies

et al. 2015

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“…Testing has revealed that IgY has a high success rate against several enteric pathogens, including bovine and human rotaviruses, enterotoxigenic Escherichia coli (ETEC), Edwardsiella tarda, bovine coronavirus, Yersinia ruckeri, Salmonella spp., Staphylococcus and Pseudomonas when administered orally. When investigated in vitro, IgY was shown to preclude infection and also to limit growth, while in animals it was observed to inhibit the symptoms of infection (Kovacs-Nolan & Mine, 2004). stability and neutralization efficacy" (Srisapoome, Punyokun, Hongprayoon, & Areechon, 2006;Punyokun, Hongprayoon, Srisapoome, & Sirinarumitr, 2013), we found that the activity of IgY was destroyed under gastric conditions which contained pepsin or a low pH value.…”

Section: Introductionmentioning

confidence: 91%

Influence of Chitosan-Alginate Microcapsules Containing Anti-Vibrio Harveyi IgY in the Gastrointestinal Tract Simulation

Punyokun¹,

Hongprayoon²,

Srisapoome³

et al. 2015

MAS

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Egg yolk immunoglobulin (IgY) is an alternative treatment for the prevention of gastrointestinal infections in pigs, cows, chickens and fish. In our previous report, we preliminarily proved that anti-V. harveyi IgY had effective potential to control luminous disease in black tiger shrimp. However, IgY activity may be reduced or destroyed by gastric conditions, particularly low pH and digestive enzymes. Therefore, it is necessary to find an effective method to preserve the therapeutic function of IgY antibodies during gastric passage. Chitosan-alginate microcapsules have been developed to protect IgY from gastric inactivation. The processing factors included different forms of chitosan and alginate, while a CaCl2 concentration and encapsulation medium was investigated. The optimum results were obtained under the following conditions: High Mw chitosan concentration 0.2% (w/v), medium viscosity alginate concentration 2% (w/v), CaCl2 concentration 0.5% (w/v). The stability of IgY in simulated gastric fluid (SGF, pH 1.2) was greatly improved by encapsulation in chitosan-alginate microcapsules, and retained greater than 90% activity after 2 h exposure to SGF. Less than 10% IgY was released upon the microcapsules' exposure to SGF for 2 h, and more than 80% IgY was released upon the microcapsules' exposure to simulated intestinal fluid (SIF, pH 6.8) for 16 h.

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“…The heavy chain has four constant domains and one variable domain. The molecular weight of IgY is about 180 kDa [40]. One hen generally lays about 290 eggs per year which represents 5 kg of yolk.…”

Section: Immunoglobulin Ymentioning

confidence: 99%

Eggs as a Very Promising Source of Biomedical and Nutraceutical Preparations: A Review

Trziszka¹,

Różański²,

Polanowski³

2013

JLS

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It is generally accepted that eggs besides being a source of everyday diet, can also be used as raw material for special food, nutraceutical, pharmaceutical and cosmetic production. The biological activity of the majority of substances presented in egg matter is related to their anti-microbial and anti-cancer properties and their immunogenic features as well. Due to the use of new generation of feed additives and techniques of immunisation of hens, the chemical composition and biological features of eggs may be modified and enriched with desired components, like n-3 fatty acids (alpha-linolenic acid (ALA), docosahexaenoic acid (DHA) phospholipids), vitamins, microelements. Egg material may also be used for the production of new biopreparations for the prophylaxis of various civilisation diseases, such as heart and cardiovascular diseases, cancers, neurogenerative disorders, metabolic bone diseases etc.. New progress in egg science allowed to discover new bioactive substances, like monomeric and dimeric cystatin and yolkin. It is necessary to develop efficient methods of isolation of biologically active substances contained in eggs, which would make it possible to produce new generation of diet supplements and biomedical products. It seems that new generation of eggs could be the best source for production of natural, high value nutraceutical supplements of diet, and also biomedical and biocosmetic preparations.

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Avian egg antibodies: basic and potential applications

Cited by 72 publications

References 172 publications

Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies

Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies

Influence of Chitosan-Alginate Microcapsules Containing Anti-Vibrio Harveyi IgY in the Gastrointestinal Tract Simulation

Eggs as a Very Promising Source of Biomedical and Nutraceutical Preparations: A Review

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