Avian Eggshell Slurry as a Dentin Desensitizing Agent: An In Vitro Assessment Using Two Techniques

Tahlawy, Aya A El; Saba, Dalia A.; Bakir, Nahed G

doi:10.5005/jp-journals-10024-3086

Cited by 5 publications

(8 citation statements)

References 33 publications

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“…Characterization of prepared CESP revealed presence of high calcium content (51.55 wt%), (Figure 2,b); which was in agreement with many studies (7,24) . Since eggshell was proven as a promising biomaterial for remineralization of dentin defects, implementation of that cost-effective product might reduce the need of other expensive sources of calcium like MTA and BD (Metal Trioxide Aggregate and Biodentin) that had reported highest recorded number of complete dentin bridge formation (11,22,30) .…”

Section: Discussionsupporting

confidence: 88%

“…Chicken eggshell powder (CESP) represents an excellent source for hydroxyapatite due to rich content of calcium in chemical forms of oxides and carbonates. Prepared CESP is considered a sustainable renewable source for biological SHAp that is cost-effective as well (6,7) . Elaborated CESP was not only applied as a biodegradable efficient bone substitute for repair of osseous defects, but also it was successfully used for remineralization of early carious tooth tissues (8,9) .…”

Section: Introductionmentioning

confidence: 99%

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Clinical Finding of Inert Collagen Sponge Saturated by Remineralizing Solution from Eggshell Powder versus Ca-OH in Indirect Pulp Capping Measured by Cone Beam Computed Tomography Radiograph

Essa

salem

hammad

2023

Egyptian Dental Journal

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Objective of the study was to evaluate the remineralizing effect of a prepared chicken eggshell powder (CESP) containing solution on human dentine and to compare it to that of light curing calcium hydroxide (Ca-OH) cavity liner. Chicken eggshell powder was prepared, characterized using a scanning electron microscope (SEM) and an energy-dispersive X-ray spectroscopy (EDX). A total of 24 patients were selected from operative dentistry clinic, each patient had at least two occlusal deep carious defects in permanent premolars or molars. After complete caries excavation, the 48 teeth were divided into two groups (n = 24): according to the received treatment: eggshell collagen delivering device (group 1) and light curing Ca-OH (group 2) as indirect direct pulp capping biomaterials. Teeth were then restored by a reinforced glass ionomer base and a permanent resin-based composite (RBC) filling. Cone beam computed tomography (CBCT) radiographs were used to evaluate and compare the variation in thickness of inducted dentine bridge immediately after insertion of final restoration and after 6months to evaluate thickness of dentine bridge finally the measurements were statistically evaluated by paired t test. Results showed that the group of teeth treated by pulp capping using the prepared remineralizing eggshell delivered by kolspon sponge showed a significantly higher thickness of dentin bridge formation than the group that was managed by application of conventional Ca-OH pulp capping agent. Concluding that A prepared chicken eggshell powder (CESP) in reminerization solution loaded into a collagen kolspon sponge might serve successfully in induction of newly formed reparative dentine bridge.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Clinical Finding of Inert Collagen Sponge Saturated by Remineralizing Solution from Eggshell Powder versus Ca-OH in Indirect Pulp Capping Measured by Cone Beam Computed Tomography Radiograph

Essa

salem

hammad

2023

Egyptian Dental Journal

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“…However, a previous study had shown that although avian chicken eggshell-derived nHAp exhibited similar DP as CPP-ACP, its tubule occluding potential and acid resistance were significantly superior to CPP-ACP. However, the authors hypothesized that nHAp could have only superficially occluded the DTs [ 14 ]. In the present study, it is evident that nHAp had formed deeper plugs into the DTs.…”

Section: Discussionmentioning

confidence: 99%

“…The integrity of TO post-remineralization can be evaluated using an acid challenge test or the DP test [ 25 ]. CA was used for the acid challenge in the present study as it is a common ingredient of fruits, fruit juices, and other beverages consumed [ 14 ]. Dias da Cruz et al used 6% CA for 2 min to study the resistance offered by desensitized dentin to acid [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…OS is composed of approximately 96% calcium carbonate, trace amounts of oxides of Na, Mg, Si, Al, and moisture [ 10 , 12 ]. Studies have shown that chicken eggshell-derived nHAp occluded patent DTs effectively and suggested that it could be a potential therapeutic agent for DH [ 13 , 14 ]. But, the remineralizing potential of nHAp derived from OS (os-nHAp) is yet to be tapped in dentistry.…”

Section: Introductionmentioning

confidence: 99%

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Oyster shell-derived nano-hydroxyapatite and proanthocyanidin pretreatment on dentinal tubule occlusion and permeability before and after acid challenge—an in vitro study

Ram

Sujatha

Vidhya

et al. 2023

J Mater Sci: Mater Med

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This in vitro study evaluated the dentinal tubule occlusion (TO), depth of penetration (DoP), and dentin permeability (DP) of oyster shell-derived nanohydroxyapatite (os-nHAp) with and without 15% proanthocyanidin (PA) pretreatment. os-nHAp was synthesized via the precipitation method and it was characterized. The morphology and particle size of os-nHAp were analyzed using a high-resolution transmission electron microscope (HRTEM). Cytotoxicity of os-nHAp, PA/os-nHAp, and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) was assessed by (3-(4,5-dimethythiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) assay using human osteosarcoma (MG-63) cell line. One hundred and ninety-seven dentin discs of 3 mm thickness were prepared from the crown portion of extracted human teeth. The dentinal surfaces of the discs were etched for 2 min with 6% citric acid to simulate dentin hypersensitivity. Five discs were randomly selected and the patency of dentinal tubules was confirmed using a scanning electron microscope (SEM). The remaining 192 discs were divided into four groups (n = 48) depending on the type of remineralization as follows: group 1: os-nHAp, group 2: PA/os-nHAp, group 3: CPP-ACP, and group 4: no treatment. The remineralization protocol was followed for 21 days. Out of the 48 dentin discs in each group, 32 discs were used to evaluate dentinal tubule occlusion (TO) and depth of penetration (DoP) using SEM. The remaining 16 discs were subjected to an assessment of dentin permeability (DP) using a hydraulic conductance model. TO, DoP and DP were evaluated after remineralization and acid challenge. Characterization studies confirmed the presence of pure phase apatite. HRTEM confirmed the nanometric particle size of os-nHAp. MTT assay results showed that all the tested materials exhibited >80% cell viability when tested up to a concentration of 100 µg/mL. The results demonstrated a significantly higher mean percentage of TO, DoP, and lesser mean DP after remineralization in groups 1, 2, and 3 (p < 0.05). After the acid challenge, group 3 showed a significant reduction in TO and DoP, and increased DP (p < 0.05). However, no such changes were observed in groups 1 and 2. Within the limitations of this study, it can be concluded that os-nHAp and PA/os-nHAp could serve as potential and durable therapeutic agents in the treatment of dentin hypersensitivity. Graphical Abstract

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Nano eggshell‐based slurry as a direct pulp‐capping material: In vitro characterization and histopathological assessment in an experimental animal model

Nabil Sulyiman,

El‐Rashidy,

El Moshy

et al. 2023

Int Endodontic J

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AimPulp vitality is essential for tooth integrity. Following pulp exposure, choosing a suitable pulp‐capping material is crucial to maintain pulp vitality. However, the reparative dentine bridge created by calcium hydroxide (Ca(OH)2) is generally porous and incomplete. The aim of the current study is to assess the in vitro and in vivo bioactivities of nano eggshell‐based slurry (NES), using NES as a direct pulp‐capping material, compared with Ca(OH)2 in rabbit animal model.MethodologyNano eggshell powder (NE) was characterized for particle morphology, chemical composition and ion release. In vitro bioactivity was tested by immersion in simulated body fluid (SBF) for 7 days. For histopathological evaluation, 36 adult New Zealand rabbits (72 pulp exposures) were divided into nine groups (n = 8) according to the pulp‐capping material (NES, Ca(OH)2 and no capping as negative control group) and the animals were sacrificed after 7, 14 or 28 days. The pulps of the two lower central incisors were exposed and then directly capped by Ca(OH)2 or NES or left untreated. The cavities were then sealed with glass ionomer cement. Teeth were collected for histopathological evaluation using an optical microscope. Pulp haemorrhage, inflammation, fibrosis and calcific bridge formation were assessed. Results were statistically analysed using anova and Tukey's tests.ResultsNano eggshell particles were spherical with a 20 nm diameter and were composed mainly of calcite. Statistical analysis showed that there was a significant increase in the release of all investigated ions between days 1 and 28, except for copper. NES group showed a significantly higher release of all elements as compared to Ca(OH)2. Environmental scanning electron microscope micrographs of NES incubated for 7 days in SBF showed the formation of HAp with a Ca/P ratio (1.686). For histopathological evaluation, the difference between groups was statistically significant. At day 28, 75% of the pulps of the Ca(OH)2 group showed mild calcific bridge in comparison with 100% moderate calcific bridge in the NES group. The NES group showed significantly less inflammation at days 7 and 28, and higher fibrosis at day 7 compared with Ca(OH)2.ConclusionsNano eggshell‐based slurry represents a promising novel direct pulp‐capping material with favourable pulp tissue response.

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Avian Eggshell Slurry as a Dentin Desensitizing Agent: An In Vitro Assessment Using Two Techniques

Cited by 5 publications

References 33 publications

Clinical Finding of Inert Collagen Sponge Saturated by Remineralizing Solution from Eggshell Powder versus Ca-OH in Indirect Pulp Capping Measured by Cone Beam Computed Tomography Radiograph

Clinical Finding of Inert Collagen Sponge Saturated by Remineralizing Solution from Eggshell Powder versus Ca-OH in Indirect Pulp Capping Measured by Cone Beam Computed Tomography Radiograph

Oyster shell-derived nano-hydroxyapatite and proanthocyanidin pretreatment on dentinal tubule occlusion and permeability before and after acid challenge—an in vitro study

Nano eggshell‐based slurry as a direct pulp‐capping material: In vitro characterization and histopathological assessment in an experimental animal model

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